Hydroxyproline: A Potential Biochemical Marker and Its Role in the Pathogenesis of Different Diseases

Srivastava, Amit; Khare, Piush; Nagar, Hemant; Raghuwanshi, Navdeep; Srivastava, Rajnish

doi:10.2174/1389203717666151201192247

Cited by 117 publications

(74 citation statements)

References 66 publications

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“…Moreover, associations with citrus fruits and juices were found for Nmethylproline and (less pronounced) for proline, but not for hydroxyproline. These results are in accordance with the fact that hydroxyproline is a degradation-product of collagen, 51,52 whereas the other proline compounds are mainly present in varying amounts in different fruits.…”

Section: Analysis Of Karmen Study Samples and Associations With Cursupporting

confidence: 89%

Targeted ultra‐performance liquid chromatography/tandem mass spectrometric quantification of methylated amines and selected amino acids in biofluids

Roggensack

Merz

Dick

et al. 2020

Rapid Comm Mass Spectrometry

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Rationale Methylated amino compounds and basic amino acids are important analyte classes with high relevance in nutrition, physical activity and physiology. Reliable and easy quantification methods covering a variety of metabolites in body fluids are a prerequisite for efficient investigations in the field of food and nutrition. Methods Targeted ultra‐performance liquid chromatography/tandem mass spectrometric (UHPLC/MS) analysis was performed using HILIC separation and timed ESI‐MRM detection, combined with a short sample preparation. Calibration in urine and blood plasma was achieved by matrix‐matched standards, isotope‐labelled internal standards and standard addition. The method was fully validated and the performance was evaluated using a subset from the Karlsruhe Metabolomics and Nutrition (KarMeN) study. Results Within this method, a total of 30 compounds could be quantified simultaneously in a short run of 9 min in both body fluids. This covers a variety of free amino compounds which are present in very different concentrations. The method is easy, precise and robust, and has a broad working range. As a proof of principle, literature‐based associations of certain metabolites with dietary intake of respective foods were clearly confirmed in the KarMeN subset. Conclusions Overall, the method turned out to be well suited for application in nutrition studies, as shown for the example of food intake biomarkers in KarMeN. Application to a variety of questions such as food‐related effects or physical activity will support future studies in the context of nutrition and health.

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Section: Analysis Of Karmen Study Samples and Associations With Cursupporting

confidence: 89%

Targeted ultra‐performance liquid chromatography/tandem mass spectrometric quantification of methylated amines and selected amino acids in biofluids

Roggensack

Merz

Dick

et al. 2020

Rapid Comm Mass Spectrometry

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“…Hydroxyproline is a quantitative biomarker of collagen (19). When all mice were compared, the hydroxyproline content was significantly higher in the lungs of the bleomycin-treated mice than in the lungs of the sham animals (Supplemental Fig.…”

Section: Collagen Quantificationmentioning

confidence: 99%

Optimization of a Collagen-Targeted PET Probe for Molecular Imaging of Pulmonary Fibrosis

et al. 2017

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There is a large unmet need for a simple, accurate, noninvasive, quantitative, and high-resolution imaging modality to detect lung fibrosis at early stage and to monitor disease progression. Overexpression of collagen is a hallmark of organ fibrosis. Here, we describe the optimization of a collagen-targeted PET probe for staging pulmonary fibrosis. Six peptides were synthesized, conjugated to a copper chelator, and radiolabeled withCu. The collagen affinity of each probe was measured in a plate-based assay. The pharmacokinetics and metabolic stability of the probes were studied in healthy rats. The capacity of these probes to detect and stage pulmonary fibrosis in vivo was assessed in a mouse model of bleomycin-induced fibrosis using PET imaging. All probes exhibited affinities in the low micromolar range (1.6 μM< Kd < 14.6 μM) and had rapid blood clearance. The probes showed 2- to 8-fold-greater uptake in the lungs of bleomycin-treated mice than sham-treated mice, whereas the distribution in other organs was similar between bleomycin-treated and sham mice. The probe Cu-CBP7 showed the highest uptake in fibrotic lungs and the highest target-to-background ratios. The superiority ofCu-CBP7 was traced to a much higher metabolic stability compared with the other probes. The specificity of Cu-CBP7 for collagen was confirmed by comparison with a nonbinding isomer.Cu-CBP7 is a promising candidate for in vivo imaging of pulmonary fibrosis.

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“…Moreover, the activation of HSC is associated with increased synthesis of hyaluronic acid (HA), an important component of the extracellular matrix. Liver fibrosis is also characterized by elevated level of hydroxyproline (HYP), an important constituent of collagen …”

Section: Introductionmentioning

confidence: 99%

Role of betaine in liver injury induced by the exposure to ionizing radiation

Shedid

Abdel‐Magied

Saada

2018

Environmental Toxicology

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Oxidative stress, apoptosis, and fibrosis may play a major role in the development of radiationinduced liver damage. Betaine, a native compound widely present in beetroot, was reported to possess hepato-protective properties. The objective of this study was to investigate the influence of betaine on radiation-induced liver damage. Animals were exposed to 9 Gy applied in 3 doses of 3 Gy/wk. Betaine (400 mg/kg/d), was orally supplemented to rats after the first radiation dose, and daily during the irradiation period. Animals were sacrificed 1 day after the last dose of radiation. The results showed that irradiation has induced oxidative stress in the liver denoted by a significant elevation in malondialdehyde, protein carbonyl, and 8-hydroxy-2-deoxyguanosine with a significant reduction in catalase activity and glutathione (GSH) content. The activity of the detoxification enzyme cytochrome P450 (CYP450) increased while GSH transferase (GSH-T) decreased. The activity of the apoptotic marker caspase-3 increased concomitant with increased hyaluronic acid, hydroxyproline, laminin (LN), and collagen IV. These alterations were associated with a significant increase of gamma-glutamyl transferase, alkaline phosphatase and alanine and aspartate aminotransferase markers of liver dysfunction. Betaine treatment has significantly attenuated oxidative stress, decreased the activity of CYP450, enhanced GSH-T, reduced the activity of caspase-3, and the level of fibrotic markers concomitant with a significant improvement of liver function. In conclusion, betaine through its antioxidant activity and by enhancing liver detoxification and reducing apoptosis may alleviate the progression of liver fibrosis and exert a beneficial impact on radiation-induced liver damage.

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Hydroxyproline: A Potential Biochemical Marker and Its Role in the Pathogenesis of Different Diseases

Cited by 117 publications

References 66 publications

Targeted ultra‐performance liquid chromatography/tandem mass spectrometric quantification of methylated amines and selected amino acids in biofluids

Targeted ultra‐performance liquid chromatography/tandem mass spectrometric quantification of methylated amines and selected amino acids in biofluids

Optimization of a Collagen-Targeted PET Probe for Molecular Imaging of Pulmonary Fibrosis

Role of betaine in liver injury induced by the exposure to ionizing radiation

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