(−)-Hydroxycitric Acid Influenced Fat Metabolism via Modulating of Glucose-6-phosphate Isomerase Expression in Chicken Embryos

Li, Shengnan; Yang, Zhongmiao; Zhang, Huihui; Peng, Mengling; Ma, Haitian

doi:10.1021/acs.jafc.9b02330

Cited by 4 publications

(3 citation statements)

References 46 publications

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“…ACACA is a key regulator of lipogenesis in the adipose tissue of animals [ 31 , 32 ]. GPI acts in the glucose pathway into pentose phosphate and produces NADPH, which is necessary for lipid metabolism [ 33 , 34 ]. In our study, ACACA and GPI were selected in the PPI network, which may reveal that upregulation of these DEGs may induce fat deposition in the Hu sheep breed.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome study digs out BMP2 involved in adipogenesis in sheep tails

Jin

Fei

et al. 2022

BMC Genomics

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Background Hu sheep and Tibetan sheep in China are characterized by fat tails and thin tails, respectively. Several transcriptomes have been conducted in different sheep breeds to identify the differentially expressed genes (DEGs) underlying this trait. However, these studies identified different DEGs in different sheep breeds. Results Hence, RNA sequencing was performed on Hu sheep and Tibetan sheep. We obtained a total of 45.57 and 43.82 million sequencing reads, respectively. Two libraries mapped reads from 36.93 and 38.55 million reads after alignment to the reference sequences. 2108 DEGs were identified, including 1247 downregulated and 861 upregulated DEGs. GO and KEGG analyses of all DEGs demonstrated that pathways were enriched in the regulation of lipolysis in adipocytes and terms related to the chemokine signalling pathway, lysosomes, and glycosaminoglycan degradation. Eight genes were selected for validation by RT–qPCR. In addition, the transfection of BMP2 overexpression into preadipocytes resulted in increased PPAR-γ expression and expression. BMP2 potentially induces adipogenesis through LOX in preadipocytes. The number of lipid drops in BMP2 overexpression detected by oil red O staining was also greater than that in the negative control. Conclusion In summary, these results showed that significant genes (BMP2, HOXA11, PPP1CC and LPIN1) are involved in the regulation of adipogenesis metabolism and suggested novel insights into metabolic molecules in sheep fat tails.

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Section: Discussionmentioning

confidence: 99%

Transcriptome study digs out BMP2 involved in adipogenesis in sheep tails

Jin

Fei

et al. 2022

BMC Genomics

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“…However, supplementation with polyunsaturated FAs can resolve this issue [ 92 ]. Other genes associated with LD function in the embryo include GPI [ 93 ], LSD [ 94 ], Myosin family [ 32 ], and other genes regulated.…”

Section: Lds-related Genes and Regulatory Functions In Embryonic Deve...mentioning

confidence: 99%

Beyond energy provider: multifunction of lipid droplets in embryonic development

Jin

Ren

2023

Biol Res

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Since the discovery, lipid droplets (LDs) have been recognized to be sites of cellular energy reserves, providing energy when necessary to sustain cellular life activities. Many studies have reported large numbers of LDs in eggs and early embryos from insects to mammals. The questions of how LDs are formed, what role they play, and what their significance is for embryonic development have been attracting the attention of researchers. Studies in recent years have revealed that in addition to providing energy for embryonic development, LDs in eggs and embryos also function to resist lipotoxicity, resist oxidative stress, inhibit bacterial infection, and provide lipid and membrane components for embryonic development. Removal of LDs from fertilized eggs or early embryos artificially leads to embryonic developmental arrest and defects. This paper reviews recent studies to explain the role and effect mechanisms of LDs in the embryonic development of several species and the genes involved in the regulation. The review contributes to understanding the embryonic development mechanism and provides new insight for the diagnosis and treatment of diseases related to embryonic developmental abnormalities.

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“…Due to their ubiquitous presence in the biological system, anions strongly impact different processes of life. Among the various anions, phosphate render remarkable role in metabolic processes [1,2] and energy transduction [3,4] . Na + , K + , Ca + , and NH 4 + salts of phosphate are well known to play foremost role in healthcare products.…”

Section: Introductionmentioning

confidence: 99%

Click‐Generated Triazole‐Based Silanes: A Potent Chemosensor for Dihydrogen Phosphate

et al. 2023

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Over the years, ground and surface water has been reported to be contaminated with phosphates. These phosphates are known to cause various diseases and if present in excess can lead to eutrophication. The known silane chemistry has not been thoroughly investigated for the sensing of anions. In the current study four new triazole based silanes have been prepared by the renowned Click Chemistry for the selective and sensitive detection of dihydrogen phosphate. The synthesized compounds were well characterized by various spectroscopic techniques and their absorptive and emissive responses were investigated for the recognition of dihydrogen phosphate ions. The binding ratio between triazole based silane chemosensor and dihydrogen phosphate has been discovered from Job's plot and was also confirmed by non‐linear fitting method. The value of association constant was found to be 571.18 M−1. The limit of detection (LOD) and limit of quantitation (LOQ) were calculated by fluorescence spectroscopy. The results obtained clearly illustrated that triazole based silane could be used as a potent chemosensor for selective and sensitive recognition of dihydrogen phosphate. Further, the possible binding site of chemosensor for dihydrogen phosphate has been identified from 1H NMR studies and DFT calculations. At last real sample analysis was performed to check the practical applicability of chemosensor.

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(−)-Hydroxycitric Acid Influenced Fat Metabolism via Modulating of Glucose-6-phosphate Isomerase Expression in Chicken Embryos

Cited by 4 publications

References 46 publications

Transcriptome study digs out BMP2 involved in adipogenesis in sheep tails

Transcriptome study digs out BMP2 involved in adipogenesis in sheep tails

Beyond energy provider: multifunction of lipid droplets in embryonic development

Click‐Generated Triazole‐Based Silanes: A Potent Chemosensor for Dihydrogen Phosphate

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