Hydroxyapatite particles as a controlled release carrier of protein

Matsumoto, Takuya; Okazaki, Masanori; Inoue, Masahiro; Yamaguchi, Satoshi; Kusunose, Takafumi; Toyonaga, Tetsuya; Hamada, Y.; Takahashi, Junzô

doi:10.1016/j.biomaterials.2003.10.081

Cited by 319 publications

(214 citation statements)

References 29 publications

(26 reference statements)

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“…However, little information is available on the release of bFGF from calcium phosphate implants. The release of bFGF from calcium phosphate crystals is difficult under conditions of physiological pH [19,24]. In this study, we performed just cell proliferation test, but not other biologic function of bFGF such like Alkaline phosphatase activity and calcification test, because an our previous examination demonstrated the osteogenesis in canine skull (the data not shown).…”

Section: Discussionmentioning

confidence: 98%

“…Although calcium phosphate is a good DDS, its capability to release growth factors may be poorer than that observed in other artificial bone materials [20]. Generally, the growth factor release rate of the DDS depends on the crystallinity of calcium phosphate, sinter temperature, Ca 2+ ion affinity, and pH of the environment surrounding the bone implants [19,27,32,36]. Dong et al reported that BMPs bind to the -OH, -NH 2 , and -COO-groups of hydroxyapatite, and that the Coulomb forces involved in these interactions and hydrogen bonding may affect the release of BMPs from the implant Fig.…”

Section: Discussionmentioning

confidence: 99%

“…In some cases, calcium phosphate may show very high affinity for the incorporated proteins, thereby preventing the release of the proteins [20]. At times, the incorporated proteins may not be released from the calcium phosphate under conditions of physiological pH, leading to a reduction in their function [19]. These findings suggest that bFGF incorporated into tailor-made bone implants made of α-TCP may not be released at an appropriate time to induce bone regeneration.…”

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confidence: 99%

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Effect of Trehalose Coating on Basic Fibroblast Growth Factor Release from Tailor-Made Bone Implants

Choi

Lee

Igawa

et al. 2011

The Journal of Veterinary Medical Science

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ABSTRACT. Artificial bone implants are often incorporated with osteoinductive factors to facilitate early bone regeneration. Calcium phosphate, the main component in artificial bone implants, strongly binds these factors, and in a few cases, the incorporated proteins are not released from the implant under conditions of physiological pH, thereby leading to reduction in their osteoinductivity. In this study, we coated tailor-made bone implants with trehalose to facilitate the release of basic fibroblast growth factor (bFGF). In an in vitro study, mouse osteoblastic cells were separately cultured for 48 hr in a medium with a untreated implant (T-), trehalose-coated implant (T+), bFGF-incorporated implant (FT-), and bFGF-incorporated implant with trehalose coating (FT+). In the FT+ group, cell viability was significantly higher than that in the other groups (P<0.05). Scanning electron microscopy (SEM) and X-ray diffraction (XRD) revealed that trehalose effectively covered the surface of the artificial bone implant without affecting the crystallinity or the mechanical strength of the artificial bone implant. These results suggest that coating artificial bone implants with trehalose could limit the binding of bFGF to calcium phosphate.KEY WORDS: artificial bone implant, bFGF, in vitro, trehalose.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Effect of Trehalose Coating on Basic Fibroblast Growth Factor Release from Tailor-Made Bone Implants

Choi

Lee

Igawa

et al. 2011

The Journal of Veterinary Medical Science

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“…These results suggested that in RN-HA coating enhanced the attachment and proliferation of WJCs, which are in vitro noncytotoxic and biocompatible with cells. This may be attributed to attributes of RN-HA coatings as compared to FM-HA coatings, such as the surface structure [18]. The higher crystallinity and density of the RN-HA coating could improve cell attachment and growth as seen with the SEM and phase-contrast microscopy.…”

Section: Discussionmentioning

confidence: 99%

Biocompatibility of nano-hydroxyapatite/Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton’s jelly in vitro

Guan

Shan

Shi

et al. 2014

Sci. China Life Sci.

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Seeding cells and scaffolds play pivotal roles in bone tissue engineering and regenerative medicine. Wharton's jelly-derived mesenchymal stem cells (WJCs) from human umbilical cord represent attractive and promising seeding cells in tissue regeneration and engineering for treatment applications. This study was carried out to explore the biocompatibility of scaffolds to seeding cells in vitro. Rod-like nano-hydroxyapatite (RN-HA) and flake-like micro-hydroxyapatite (FM-HA) coatings were prepared on Mg-Zn-Ca alloy substrates using micro-arc oxidation and electrochemical deposition. WJCs were utilized to investigate the cellular biocompatibility of Mg-Zn-Ca alloys after different surface modifications by observing the cell adhesion, morphology, proliferation, and osteoblastic differentiation. The in vitro results indicated that the RN-HA coating group was more suitable for cell proliferation and cell osteoblastic differentiation than the FM-HA group, demonstrating better biocompatibility. Our results suggested that the RN-HA coating on Mg-Zn-Ca alloy substrates might be of great potential in bone tissue engineering.nano-hydroxyapatite, Mg-Zn-Ca alloy, mesenchymal stem cells, Wharton's jelly, biocompatibility Citation:Guan FX, Ma SS, Shi XY, Ma X, Chi LK, Liang S, Cui YB, Wang ZB, Yao N, Guan SK, Yang B. Biocompatibility of nano-hydroxyapatite/ Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton's jelly in vitro.

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“…In contrast, the microcompressive strength of individual HApS particles is only about 0.6 MPa, which is extremely low compared to the value of approximately 180 MPa for fluoroaluminosilicate glass particles 20) . In addition, HAp also has the ability to not only adsorb but also release several substances 29,30) . Shiozawa et al 31) demonstrated that Ca enhanced the formation of GIC matrix, as a results, the surface hardness of GIC increased.…”

Section: Discussionmentioning

confidence: 99%

Effects of porous-hydroxyapatite incorporated into glass-ionomer sealants

et al. 2015

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The purpose of the present study was to evaluate the mechanical and chemical properties of a novel glass ionomer cement for use as a pit and fissure sealant containing a porous hydroxyapatite, namely, apatite ionomer cement (AIC). Control sealant samples were used Fuji III (GIC-S). The experiment sealant samples (AIC-S) consisted of porous spherical hydroxyapatite (HApS) particles added at 28 wt% to GIC-S powder. The GIC-S and AIC-S samples were evaluated through mechanical strength measurements, scanning electron microscopy observations, energy dispersive X-ray spectroscopy analysis, fluoride ion release tests, and antibacterial tests. The flexural strength of the AIC-S was significantly higher than that of GIC-S for each period, 1 h, 24 h and 1 year. The fluoride release dose for AIC-S was consistently higher than that for GIC-S. In addition, the antibacterial properties of AIC-S were superior to those of GIC-S. The novel AIC-S may be a more suitable sealant material for pits and fissures with intact and/or infected enamel.

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Hydroxyapatite particles as a controlled release carrier of protein

Cited by 319 publications

References 29 publications

Effect of Trehalose Coating on Basic Fibroblast Growth Factor Release from Tailor-Made Bone Implants

Effect of Trehalose Coating on Basic Fibroblast Growth Factor Release from Tailor-Made Bone Implants

Biocompatibility of nano-hydroxyapatite/Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton’s jelly in vitro

Effects of porous-hydroxyapatite incorporated into glass-ionomer sealants

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