Biocompatibility of nano-hydroxyapatite/Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton’s jelly in vitro

Guan, Fang Xia; Shan, Shan; Shi, Xin; Zhang, Fujun; Lian, Kai; Liang, Shuo; Cui, Yuan Bo; Wang, Zhi Bin; Yao, Ning; Guan, Shao Kang; Yang, Bo

doi:10.1007/s11427-014-4610-9

Cited by 12 publications

(4 citation statements)

References 35 publications

(24 reference statements)

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“…Because the Mg-Zn-Ca alloy has excellent mechanical and biocompatibility properties, it may serve as a material for bone implants in the future [ 23 – 26 ]. However, a drawback to its use is that its corrosion rate cannot be effectively controlled once placed in a body.…”

Section: Discussionmentioning

confidence: 99%

Assessment of the degradation rates and effectiveness of different coated Mg-Zn-Ca alloy scaffolds for in vivo repair of critical-size bone defects

Zhang

Zhao

Liu

et al. 2018

J Mater Sci: Mater Med

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Surgical repair of bone defects remains challenging, and the search for alternative procedures is ongoing. Devices made of Mg for bone repair have received much attention owing to their good biocompatibility and mechanical properties. We developed a new type of scaffold made of a Mg-Zn-Ca alloy with a shape that mimics cortical bone and can be filled with morselized bone. We evaluated its durability and efficacy in a rabbit ulna-defect model. Three types of scaffold-surface coating were evaluated: group A, no coating; group B, a 10-μm microarc oxidation coating; group C, a hydrothermal duplex composite coating; and group D, an empty-defect control. X-ray and micro-computed tomography(micro-CT) images were acquired over 12 weeks to assess ulnar repair. A mechanical stress test indicated that bone repair within each group improved significantly over time (P < 0.01). The degradation behavior of the different scaffolds was assessed by micro-CT and quantified according to the amount of hydrogen gas generated; these measurements indicated that the group C scaffold better resisted corrosion than did the other scaffold types (P < 0.05). Calcein fluorescence and histology revealed that greater mineral densities and better bone responses were achieved for groups B and C than for group A, with group C providing the best response. In conclusion, our Mg-Zn-Ca-alloy scaffold effectively aided bone repair. The group C scaffold exhibited the best corrosion resistance and osteogenesis properties, making it a candidate scaffold for repair of bone defects.

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Section: Discussionmentioning

confidence: 99%

Assessment of the degradation rates and effectiveness of different coated Mg-Zn-Ca alloy scaffolds for in vivo repair of critical-size bone defects

Zhang

Zhao

Liu

et al. 2018

J Mater Sci: Mater Med

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“…Nano-HA/chitosan/PLGA scaffolds induced human UC-MSCs to differentiate into osteoblasts for the repair of calvarial defects in nude mice. 351–353 …”

Section: Interactions Between Bone Biomaterials and Stem Cellsmentioning

confidence: 99%

Bone biomaterials and interactions with stem cells

et al. 2017

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Bone biomaterials play a vital role in bone repair by providing the necessary substrate for cell adhesion, proliferation, and differentiation and by modulating cell activity and function. In past decades, extensive efforts have been devoted to developing bone biomaterials with a focus on the following issues: (1) developing ideal biomaterials with a combination of suitable biological and mechanical properties; (2) constructing a cell microenvironment with pores ranging in size from nanoscale to submicro- and microscale; and (3) inducing the oriented differentiation of stem cells for artificial-to-biological transformation. Here we present a comprehensive review of the state of the art of bone biomaterials and their interactions with stem cells. Typical bone biomaterials that have been developed, including bioactive ceramics, biodegradable polymers, and biodegradable metals, are reviewed, with an emphasis on their characteristics and applications. The necessary porous structure of bone biomaterials for the cell microenvironment is discussed, along with the corresponding fabrication methods. Additionally, the promising seed stem cells for bone repair are summarized, and their interaction mechanisms with bone biomaterials are discussed in detail. Special attention has been paid to the signaling pathways involved in the focal adhesion and osteogenic differentiation of stem cells on bone biomaterials. Finally, achievements regarding bone biomaterials are summarized, and future research directions are proposed.

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“…Compared with flake‐like micro‐hydroxyapatite, rod‐like nano‐hydroxyapatite deposited on a Mg‐Zn‐Ca alloy coated with MgO arranged neatly in a consistent direction promotes MSC adhesion and migration on implants. [ 95 ] In addition, nanoscale surfaces guide the assembly of integrin, a cell adhesion protein aggregate that facilitates the synthesis of intracellular tight junctions and promotes the adhesion and osteoblastic differentiation of BMSCs. [ 96 ] A double‐layer phytic acid@cerium/polycaprolactone (PCL)@cerium‐substituted hydroxyapatite composite nanocoating with a high aspect ratio of nanofiber structures on an AZ31 Mg alloy provided an ECM‐like interface, which induced BMSCs to express endogenous hyalin to link integrin clusters and form larger and more mature pseudopods on the nanosurface, thus promoting cellular proliferation and differentiation.…”

Section: Mechanisms Of Composite Nanocoatings To Improve the Utility ...mentioning

confidence: 99%

Composite Nanocoatings of Biomedical Magnesium Alloy Implants: Advantages, Mechanisms, and Design Strategies

Dai

Xiong

et al. 2023

Advanced Science

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The rapid degradation of magnesium (Mg) alloy implants erodes mechanical performance and interfacial bioactivity, thereby limiting their clinical utility. Surface modification is among the solutions to improve corrosion resistance and bioefficacy of Mg alloys. Novel composite coatings that incorporate nanostructures create new opportunities for their expanded use. Particle size dominance and impermeability may increase corrosion resistance and thereby prolong implant service time. Nanoparticles with specific biological effects may be released into the peri‐implant microenvironment during the degradation of coatings to promote healing. Composite nanocoatings provide nanoscale surfaces to promote cell adhesion and proliferation. Nanoparticles may activate cellular signaling pathways, while those with porous or core–shell structures may carry antibacterial or immunomodulatory drugs. Composite nanocoatings may promote vascular reendothelialization and osteogenesis, attenuate inflammation, and inhibit bacterial growth, thus increasing their applicability in complex clinical microenvironments such as those of atherosclerosis and open fractures. This review combines the physicochemical properties and biological efficiency of Mg‐based alloy biomedical implants to summarize the advantages of composite nanocoatings, analyzes their mechanisms of action, and proposes design and construction strategies, with the purpose of providing a reference for promoting the clinical application of Mg alloy implants and to further the design of nanocoatings.

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Biocompatibility of nano-hydroxyapatite/Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton’s jelly in vitro

Cited by 12 publications

References 35 publications

Assessment of the degradation rates and effectiveness of different coated Mg-Zn-Ca alloy scaffolds for in vivo repair of critical-size bone defects

Assessment of the degradation rates and effectiveness of different coated Mg-Zn-Ca alloy scaffolds for in vivo repair of critical-size bone defects

Bone biomaterials and interactions with stem cells

Composite Nanocoatings of Biomedical Magnesium Alloy Implants: Advantages, Mechanisms, and Design Strategies

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