Hydroxyapatite and a New Fibrin Sealant Derived from Snake Venom as Scaffold to Treatment of Cranial Defects in Rats

Cunha, Marcelo Rodrigues da; Menezes, Fernanda Araujo; Santos, Geovane Ribeiro dos; Pinto, Clóvis Antônio Lopes; Barraviera, Benedito; Martins, Virgínia da Conceição Amaro; Plepis, Ana Maria de Guzzi; Ferreira, Rui Seabra

doi:10.1590/1516-1439.316014

Cited by 30 publications

(38 citation statements)

References 35 publications

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“…Only qualitative data extracted from each study were synthesized in analytic tables. In seven of the 13 included studies, New Zealand rabbits were used [16,[115][116][117][118][119][120], while six studies were conducted in rats, of which three used the Sprague-Dawley strain [17,121,122], two the Wistar strain [123,124] and one the Lewis strain [125]. The calvarial critical-sized defect was the most used model for assessing new bone formation (Table 3).…”

Section: Study Characteristicsmentioning

confidence: 99%

The Impact of Bioceramic Scaffolds on Bone Regeneration in Preclinical In Vivo Studies: A Systematic Review

et al. 2020

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Bioceramic scaffolds are appealing for alveolar bone regeneration, because they are emerging as promising alternatives to autogenous and heterogenous bone grafts. The aim of this systematic review is to answer to the focal question: in critical-sized bone defects in experimental animal models, does the use of a bioceramic scaffolds improve new bone formation, compared with leaving the empty defect without grafting materials or using autogenous bone or deproteinized bovine-derived bone substitutes? Electronic databases were searched using specific search terms. A hand search was also undertaken. Only randomized and controlled studies in the English language, published in peer-reviewed journals between 2013 and 2018, using critical-sized bone defect models in non-medically compromised animals, were considered. Risk of bias assessment was performed using the SYRCLE tool. A meta-analysis was planned to synthesize the evidence, if possible. Thirteen studies reporting on small animal models (six studies on rats and seven on rabbits) were included. The calvarial bone defect was the most common experimental site. The empty defect was used as the only control in all studies except one. In all studies the bioceramic materials demonstrated a trend for better outcomes compared to an empty control. Due to heterogeneity in protocols and outcomes among the included studies, no meta-analysis could be performed. Bioceramics can be considered promising grafting materials, though further evidence is needed.

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Section: Study Characteristicsmentioning

confidence: 99%

The Impact of Bioceramic Scaffolds on Bone Regeneration in Preclinical In Vivo Studies: A Systematic Review

et al. 2020

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“…The captive reproduction of Crotalus durissus terrificus is an important practice once their venom is a natural source of bioactive substances with therapeutic potential, such as antitumoral properties, as well as for antiophidic serum production (Cura et al, 2002;Soares et al, 2010;Calvete et al, 2011;Kumar et al, 2014;Nudel et al, 2012). The search for new drugs has indicated toxins from Crotalus durissus terrificus venom as inhibitors of cell adhesion, cell migration, epidermal tumor growth factor, metastases induced in experimental mice models, and use of some specific proteins for production of drugs (Cura et al, 2002;Cunha et al, 2015;Neves et al, 2015;Reeks et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Hormonal and ultrasonographic characterization of the seasonal reproductive cycle of male and female Crotalus durissus terrificus

Matayoshi¹,

Souza²,

Gasparotto³

et al. 2018

Anim Reprod

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Research concerning to characterize seasonal reproductive cycle in males and females of Crotalus durissus terrificus by ultrasound and hormonal measurement. Reproductive aspects (follicular and testicular cycles, and pregnancy) from 28 adult snakes (14 males and 14 females) during different months of the years were studied. Snakes housed individually in cages in an environment with controlled luminosity and humidity, and fed monthly. Females were examined by ultrasound during quiescence and active follicular phase, and pregnancy for follicular and embryo/fetal development. Males were evaluated to testicular echotexture and measurements during reproductive and non-reproductive season. The blood samples were collected from males and females for serum testosterone and progesterone determination, respectively. In 77% males the testes were identified by ultrasound and found increased size during summer, decreased serum testosterone in winter, and positive correlation between serum testosterone and testes size. There was no change in testicular echotexture in according to season. Testosterone concentration was decreased during winter and it was positively correlated with testes size. In 71% females, were observed follicular development (vitellogenesis) and gestation since winter to spring by ultrasonography. Parturition occurred mainly in summer. Pregnancy length was 123.0 ± 11.4 days, with mean 6.9 ± 1.5 newborns/female, and there was gradual increase of serum progesterone during this period. There was no variation in progesterone concentration in nongravid females. Males and females Tropical Rattlesnake show seasonal variation of reproductive cycle and was clear a biennial cycle in female. The ultrasonography can be considered an essential tool to accomplish the follicular development, pregnancy and testicular alterations in Tropical Rattlesnake.

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“…Next, a defect measuring 5 mm in diameter and 1 mm in thickness was created surgically in the left parietal bone of the skullcap, preserving the dura mater. After surgery, the periosteum, soft tissues and skin were repositioned and sutured with 6.0 silk suture, and the animals received routine postoperative care 18 .…”

Section: Experimental Designmentioning

confidence: 99%

In vivo Study of the Osteoregenerative Potential of Polymer Membranes Consisting of Chitosan and Carbon Nanotubes

et al. 2017

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Biomaterials with the hydroxyapatite and biopolymers such as chitosan derived of crustaceans are is an alternative for bone repair. Carbon nanotubes have been a focus of interest because they can ameliorate the biomechanical properties of biomaterials. The objective of this study was to evaluate these materials in the repair of cranial defects in rats. The animals were divided in groups: without implant (G1), implanted with the chitosan/carbon nanotube membrane (G2), and chitosan/nanotube membrane mineralized with hydroxyapatite (G3). The animals were sacrificed 5 weeks after surgery and the skulls were removed for analysis of the defect area. The results showed absence of chronic inflammatory and little bone neoformation in the defect area of all groups. In G2 and G3 there was lack of reabsorption of the biomaterial that were encapsulated by connective tissue. In conclusion, the biomaterials were biocompatible, but their specific physicochemical properties did not indicate a considerable osteoregenerative capacity.

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Hydroxyapatite and a New Fibrin Sealant Derived from Snake Venom as Scaffold to Treatment of Cranial Defects in Rats

Cited by 30 publications

References 35 publications

The Impact of Bioceramic Scaffolds on Bone Regeneration in Preclinical In Vivo Studies: A Systematic Review

The Impact of Bioceramic Scaffolds on Bone Regeneration in Preclinical In Vivo Studies: A Systematic Review

Hormonal and ultrasonographic characterization of the seasonal reproductive cycle of male and female Crotalus durissus terrificus

In vivo Study of the Osteoregenerative Potential of Polymer Membranes Consisting of Chitosan and Carbon Nanotubes

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