Hydrothermal Synthesis of Zinc-Incorporated Nano-Cluster Structure on Titanium Surface to Promote Osteogenic Differentiation of Osteoblasts and hMSCs

Abstract

In this study, a novel modification strategy was established to synthesize a zinc-incorporated nano-cluster structure on titanium surface in a two-step hydrothermal reaction, and the osteogenic differentiation of osteoblasts and human bone marrow mesenchymal cells (hMSCs) was studied in the presence of this synthesized nanostructure. Analyses of the surface topography and elemental composition revealed that the zinc-containing cluster-like nanostructure was successfully prepared on the titanium surface. By altering the reaction time, three surface modifications were established. The three modified titanium surfaces had improved hydrophilicity and could continuously release zinc ions in a controlled manner. In vitro study displayed that three modified titanium surfaces, especially the samples prepared by reacting for 15 min, exhibited enhanced cell adhesion, proliferation, and osteogenic differentiation compared to the pure titanium surface. The study therefore conclude that the zinc-incorporated nano-cluster modification of titanium surface through a simple procedure can establish an enhanced osteogenic microenvironment and exhibit a potential strategy of titanium surface modification to accelerate the dental implant osseointegration.

Effects of Zinc Ions Released From Ti-NW-Zn Surface on Osteogenesis and Angiogenesis In Vitro and in an In Vivo Zebrafish Model

“…In accordance with some reports and our previous studies ( Ming et al, 2017 ; Polo-Montalvo et al, 2021 ; Tang et al, 2021 ), Ti-NW-Zn samples were immersed in 6-well plates (2 ml PBS/well) at 37°C for 1 h and 1, 4, and 7 d. The concentrations of zinc ions in the PBS solutions were quantified using a Zinc Assay Kit (E011-1-1; Nanjing Jiancheng Bioengineering Institute, Nanjing, China). The absorbance was measured using a microplate reader (SpectraMax 190, MD, United States) at 630 nm.…”

Differently Charged P (VDF-TrFE) Membranes Influence Osteogenesis Through Differential Immunomodulatory Function of Macrophages

“…The CM from macrophage-negatively P (VDF-TrFE) is characterized by M2-anti-inflammatory properties as mentioned above, thus synergistically enhancing the effect of the negatively P (VDF-TrFE) on rBMSC spreading. The CM from M2 macrophages has been reported to enhance the secretion of extracellular matrix (ECM) components, especially COL-1 and integrin β1 proteins, thereby promoting rBMSC attachment and differentiation reflected by cell spreading (He et al, 2018;Peng et al, 2020;Tang et al, 2021). Moreover, the osteogenic differentiation induced by differently charged surfaces-macrophage CM was further confirmed by the mineralized nodule, ALP staining and ALP gene expression (Salamanca et al, 2021).…”

Surface Modification and Functionalities for Titanium Dental Implants

“…In addition, the surface state of the Ti implant before the reaction, the temperature, pressure, and time during the reaction will affect the final coating properties. As shown in Figure A, Tang et al fabricated a zinc incorporated nanocluster structure on the Ti surface by a two-step hydrothermal reaction . By controlling the reaction time, they obtained Zn-NC with three different morphologies, as shown in Figure B and C. All three coatings improved the hydrophilicity and surface energy of Ti implants and the sustained release of Zn ions.…”

“…(A) Process of the hydrothermal preparation of the Zn-incorporated nanocluster (Zn-NC) on the surface of Ti; (B) SEM images of Zn-NC on the surface of Ti with different reaction times; (C) Contact angle, surface energy, concentration of zinc ion released, and protein adsorption of the Zn-NC coating. Reproduced from ref . Copyright 2021, Frontiers Media S.A.…”