Hydroponic experiment for identification of tolerance traits developed by rice Nagina 22 mutants to low-phosphorus in field condition

Panigrahy, Madhusmita; Rao, D. N.; Yugandhar, Poli; Raju, N. Sravan; Krishnamurthy, P.; Voleti, S. R.; Reddy, G. Ashok; Mohapatra, Trilochan; Robin, S.; Singh, Awtar; Singh, Kuldip; Sheshshayee, M. S.; Sharma, Rameshwar; Neelamraju, Sarla

doi:10.1080/03650340.2013.821197

Cited by 29 publications

(35 citation statements)

References 25 publications

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“…There are very few studies on identification or development of rice cultivars tolerant to low P conditions at field level as maintenance and preparation of a field with low phosphate needs much more attention than a normal field and also due to lack of proper screening methods, for traits that confer low P tolerance (Panigrahy et al, 2014). Aluwihare et al (2016) selected low P tolerant rice genotypes by growing plants in pots filled with soil, collected from cultivated field without P fertilizer application for past 40 years.…”

Section: Discussionmentioning

confidence: 99%

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Identifying markers associated with yield traits in Nagina22 rice mutants grown in low phosphorus field or in alternate wet/dry conditions

Yugandhar¹,

Basava²,

Subrahmanyam³

et al. 2017

Aust J Crop Sci

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Mutants are powerful genetic resources in plant breeding and functional genomics studies. Sixty seven stable ethyl methane sulphonate (EMS) induced rice mutants and the wild type parent Nagina 22 (N22) were characterized for plant height, tiller number, panicle number and grain yield under normal, low P field and alternate wet and dry (AWD) conditions in the same season. They were also genotyped with 44 SSR markers and four Pup1 (Phosphorus uptake1) gene specific markers. Genetic diversity was analysed by combining phenotype and marker data using Ward-MLM method. Single marker analysis showed significant association of four markers RM19696, RM263, RM3688 and RM1942 with grain yield in all three conditions. K-1, a Pup1 gene specific marker was significantly associated with tiller number only under low P conditions. The average dissimilarity between mutants was 0.86 and cophenetic correlation coefficient was 0.74. Six mutants were selected as gain-of-function mutants as they showed significantly higher grain yield in all three conditions, compared with N22. The selected mutants are an important resource for gene discovery for enhanced tolerance to low P and water stress conditions and associated markers can be useful in marker assisted selection.

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Section: Discussionmentioning

confidence: 99%

“…The low P plot was developed by not giving any P fertilizers for the last 27 years, but supplied with 100 kg N ha -1 and 60 kg K ha -1 each season (Panigrahy et al, 2014). Plants were grown in 3 replications of 2 lines of each genotype and 22 plants per line with spacing of 20 cm × 20 cm in each treatment.…”

Section: Field Experimentsmentioning

confidence: 99%

Identifying markers associated with yield traits in Nagina22 rice mutants grown in low phosphorus field or in alternate wet/dry conditions

Yugandhar¹,

Basava²,

Subrahmanyam³

et al. 2017

Aust J Crop Sci

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“…One of the upland indica ( aus ) rice accession, Nagina 22, has been induced with EMS (ethyl methane sulfonate) mutagen to generate and characterize 22,292 mutant lines through an Indian National initiative involving several research institutes (Mohapatra et al 2014 ). These mutant lines were phenotyped with a collaborative effort for a range of traits, which led to identify mutants for flowering, maturity, grain number and size, plant growth and architecture, yield, resistance to blast and bacterial leaf blight diseases, tolerance to drought, heat, salinity and herbicide and phosphorus use efficiency (Ashokkumar et al 2013 ; Kulkarni et al 2013 ; Poli et al 2013 ; Mohapatra et al 2014 ; Panigrahy et al 2014 ; Lima et al 2015 ). These mutant repositories could serve as a valuable resource for mining of novel functional alleles regulating qualitative and quantitative traits for genetic improvement in rice.…”

Section: Reviewmentioning

confidence: 99%

Rice Improvement Through Genome-Based Functional Analysis and Molecular Breeding in India

Agarwal

Parida

Raghuvanshi

et al. 2016

Rice

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Rice is one of the main pillars of food security in India. Its improvement for higher yield in sustainable agriculture system is also vital to provide energy and nutritional needs of growing world population, expected to reach more than 9 billion by 2050. The high quality genome sequence of rice has provided a rich resource to mine information about diversity of genes and alleles which can contribute to improvement of useful agronomic traits. Defining the function of each gene and regulatory element of rice remains a challenge for the rice community in the coming years. Subsequent to participation in IRGSP, India has continued to contribute in the areas of diversity analysis, transcriptomics, functional genomics, marker development, QTL mapping and molecular breeding, through national and multi-national research programs. These efforts have helped generate resources for rice improvement, some of which have already been deployed to mitigate loss due to environmental stress and pathogens. With renewed efforts, Indian researchers are making new strides, along with the international scientific community, in both basic research and realization of its translational impact.

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“…The third challenge is apparent 'lack' of appropriate high NuUE donors because of fewer past efforts to identify rice germplasm accessions with high NuUE. As a result, there have been few well-documented breeding efforts for developing rice cultivars tolerant to nitrogen and phosphorus deficiencies [21,22,23,24]. In this respect, results from this study provided at least partial answers to the three practical questions and demonstrated an innovative breeding method involving four consecutive rounds of selections under six different NuUE conditions for developing rice varieties with significantly improved NuUE.…”

Section: Discussionmentioning

confidence: 84%

Developing Green Super Rice Varieties with High Nutrient Use Efficiency by Phenotypic Selection Under Varied Nutrient Conditions

Jewel¹,

Ali²,

Pang³

et al. 2018

Preprint

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To develop green super rice varieties with high and stable yields under the rainfed conditions and improved nutrient use efficiency (NuUE), a modified backcross (BC) breeding approach was adopted using a high yielding and widely adaptable Xian variety, WTR1, as the recipient and a Geng variety, HAN, as the donor. Starting from the BC1F2 generation, the BC population had gone through one generation of selection under the IG, LI and RF conditions, followed by consecutive four generations of screening and selection for high GY under six different nutrient conditions, leading to the development of 230 BC1F6 introgression lines (ILs). The final evaluation of the 230 ILs under the six nutrient conditions identified many ILs with improved yields under various combinations of nutrient deficient conditions, including 12 promising lines that had significantly improved NuUE under two or more nutrient deficiency conditions. Our results demonstrated an efficient inter-subspecific BC breeding procedure with first round selection under the rainfed-drought condition followed by four generations of progeny testing for yield performances under six different nutrient conditions. The promising ILs were studied under replicated yield trials under 75N and -NPK conditions for developing high yield rice varieties with improved NuUE. Our results indicated that NuUE in rice was controlled by complex genetic and physiological mechanisms and the developed ILs provided useful materials for genetic and molecular dissection of NuUE in rice.

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Hydroponic experiment for identification of tolerance traits developed by rice Nagina 22 mutants to low-phosphorus in field condition

Cited by 29 publications

References 25 publications

Identifying markers associated with yield traits in Nagina22 rice mutants grown in low phosphorus field or in alternate wet/dry conditions

Identifying markers associated with yield traits in Nagina22 rice mutants grown in low phosphorus field or in alternate wet/dry conditions

Rice Improvement Through Genome-Based Functional Analysis and Molecular Breeding in India

Developing Green Super Rice Varieties with High Nutrient Use Efficiency by Phenotypic Selection Under Varied Nutrient Conditions

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