Hydrophobicity of bacteria Zymomonas mobilis under varied environmental conditions

Zikmanis, Pēteris; Shakirova, L. Sh.; Auzina, Lilija; Andersone, Inara

doi:10.1016/j.procbio.2007.01.002

Cited by 31 publications

(26 citation statements)

References 28 publications

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“…1, 2) of the same kind as reported previously [37]. In fact, increased CSH indices were attained for Z. mobilis grown at elevated temperatures or concentration of the carbon source (glucose, sucrose) in the medium and the use of sucrose instead of glucose promoted the formation of more hydrophobic cells [37].…”

Section: Resultssupporting

confidence: 72%

“…However, mentioned apparent absence of KDO might be due to the elevated cultivation temperature (37°C) employed in this study [32], which could substantially reduce the amounts of LPS and, hence, KDO level in more hydrophobic [37] cells, possibly, below the limits of analytical determination. Besides, a varied KDO content could be considered as a strain-speciWc feature of particular proteobacteria [16].…”

Section: Discussionmentioning

confidence: 87%

“…We have recently reported [37] the changes in CSH of Z. mobilis 113S in response to varied environmental conditions (temperature and phase of growth, concentration or type of carbon source, etc. ), as well as an implication to the impaired barrier function for more hydrophobic cells.…”

Section: Ethanologenicmentioning

confidence: 99%

“…Zymomonas mobilis strain 113S [3] was maintained and cultivated batch wise without aeration as described previously [37].The independent cultivations were performed at diVerent temperatures (20-40°C) or at varied concentrations (0.08-0.56 M) of glucose (sucrose) as a sole carbon source in order to modify the cell surface hydrophobicity (CSH) of Z. mobilis as reported previously [37].…”

Section: Strain and Culture Conditionsmentioning

confidence: 99%

“…Microbial adherence to hydrocarbon (MATH) method [1] using o-xylene (Sigma) as described previously [37].…”

Section: Preparation Of Cells Incubation Conditions and Measurementsmentioning

confidence: 99%

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Relationship between the cell surface hydrophobicity and survival of bacteria Zymomonas mobilis after exposures to ethanol, freezing or freeze-drying

Shakirova

Auzina

Grūbe

et al. 2008

J Ind Microbiol Biotechnol

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An inverse linear relationship (P < 0.01) was detected between the cell surface hydrophobicity (CSH) and survival of ethanologenic bacteria Zymomonas mobilis 113S exposed to elevated (2.55 M) ethanol concentration. In the same way, viable cell counts of relatively hydrophobic Z. mobilis were less diminished by growing (0.85-3.40 M) ethanol concentrations as compared to more hydrophobic bacteria. Very similar inverse relationships (P < 0.01) were observed between the CSH of intact Z. mobilis and survival of cells subjected to subsequent freeze-drying or freezing/thawing cycles thereby affinity substantially lowered ability of hydrophobic bacteria to survive under adverse environments. Observed relationships were supported by significant correlations between independent analytical data of the carbohydrate content within fractions of lipopolysaccharide and surface proteins extracted from cells of varied hydrophobicity. The results suggest that the CSH could be of value to predict the ability of intact bacteria to endure stress conditions and should be monitored towards lower values during cultivation in order to reduce subsequent unwanted structural and physiological disturbances provoked by multiple stress factors.

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Section: Resultssupporting

confidence: 72%

Section: Discussionmentioning

confidence: 87%

Section: Ethanologenicmentioning

confidence: 99%

Section: Strain and Culture Conditionsmentioning

confidence: 99%

“…Microbial adherence to hydrocarbon (MATH) method [1] using o-xylene (Sigma) as described previously [37].…”

Section: Preparation Of Cells Incubation Conditions and Measurementsmentioning

confidence: 99%

See 3 more Smart Citations

Relationship between the cell surface hydrophobicity and survival of bacteria Zymomonas mobilis after exposures to ethanol, freezing or freeze-drying

Shakirova

Auzina

Grūbe

et al. 2008

J Ind Microbiol Biotechnol

Self Cite

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Biodegradation of fenpropathrin by Rhodopseudomonas sp. strain PSB07‐21 cultured under three different growth modes

et al. 2019

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This study aimed at the biodegradation of fenpropathrin by Rhodopseudomonas sp. strain PSB07‐21 cultured under different growth modes. The biomass production, cell surface hydrophobicity and fenpropathrin biodegradation efficiency of the strain PSB07‐21 cultured under the photoheterotrophic growth mode were better than that shown by the strain PSB07‐21 cultured under the photoautotrophic or the chemotrophic growth mode. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis analysis using cell‐free protein extracts showed several distinct protein bands in the gels representing the strain PSB07‐21 cultured under the photoheterotrophic growth mode. The fenpropathrin enzymatic degradation was clearly affected the bacterial growth mode. Results obtained from this study should improve our knowledge regarding fenpropathrin biodegradation under field conditions. Our findings can also be used to optimize the usage of Rhodopseudomonas sp. PSB07‐21 in field applications.

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Long-term influence of the presence of a non-aqueous phase on the cell surface hydrophobicity of Pseudomonas in two-phase partitioning bioreactors

Hernández

Muñoz

2010

Appl Microbiol Biotechnol

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The long-term influence of silicone oil 200 cSt (SO200) and 2, 2, 4, 4, 6, 8, 8-heptamethylnonane (HMN) on the cell surface hydrophobicity (CSH) of a hexane-degrading Pseudomonas aeruginosa strain and a toluene-degrading Pseudomonas putida strain was assessed in two-phase partitioning bioreactors under batch and continuous operation. CSH was evaluated using a modified BATH method based on optical density (CSH(OD)) and colony-forming unit (CSH(CFU)) measurements. In the presence of HMN, P. aeruginosa turned hydrophobic over the time course as shown by the gradual increase in CSH(OD) (61 ± 1%) and CSH(CFU) (53 ± 3%) under batch degradation and in CSH(OD) (49 ± 0%) under continuous operation. However, P. putida turned hydrophobic only under continuous operation ([Formula: see text]). On the other hand, no significant CSH enhancement was observed in both Pseudomonas strains in the presence of SO200. These results suggested that CSH is species, non-aqueous phase, and cultivation mode dependant, and an inducible property of bacteria. Maximum hexane elimination capacities increased by 2 and 3 in the presence of SO200 and HMN, respectively. Based on the absence of CSH in P. aeruginosa in the presence of SO200, the higher elimination capacities recorded were likely due to an improved hexane mass transfer (physical effect). However, in the presence of HMN, a direct hexane uptake from the non-aqueous phase (biological effect) might have also contributed to this enhancement.

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Hydrophobicity of bacteria Zymomonas mobilis under varied environmental conditions

Cited by 31 publications

References 28 publications

Relationship between the cell surface hydrophobicity and survival of bacteria Zymomonas mobilis after exposures to ethanol, freezing or freeze-drying

Relationship between the cell surface hydrophobicity and survival of bacteria Zymomonas mobilis after exposures to ethanol, freezing or freeze-drying

Biodegradation of fenpropathrin by Rhodopseudomonas sp. strain PSB07‐21 cultured under three different growth modes

Long-term influence of the presence of a non-aqueous phase on the cell surface hydrophobicity of Pseudomonas in two-phase partitioning bioreactors

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