Hydrophobic poly (amino acid) modified PEI mediated delivery of rev-casp-3 for cancer therapy

miRNA oligonucleotide and sponge for miRNA-21 inhibition mediated by PEI-PLL in breast cancer therapy

“…Polyethylenimine (PEI) is one of the most widely utilized and studied synthetic material for DNA or siRNA delivery [15][16][17]. As a non-viral delivery system, the cationic PEI could condense the negatively charged nucleic acids by electrostatic interaction [3].…”

Charge-conversional zwitterionic copolymer as pH-sensitive shielding system for effective tumor treatment

“…Luciferase plasmid (pGL3-control), cell lysates, and luciferase reporter gene assay kit were purchased from Promega (Wisconsin, USA). Rev-casp-3 gene was subcloned into a pKH3 vector and designed according to our previously reported method [24]. All other chemicals were purchased from Sinopharm Chemical reagent Co. Ltd. (China) without further treatment.…”

“…After washing in distilled water, the deparaffinized slides were boiled for 5 min in 10 mM citrate (pH 6.0) and cooled for another 5 min for antigen retrieval. Then the deparaffinized slides were incubated with antibody at 4°C overnight, and then stained with a streptavidin biotin system and Aqua-tex according to the manufacturer's protocol [19,24].…”

“…The shielding system (OEAL) consisted of oligoethylenimine (OEI), poly(L-aspartate) (PBLA), and poly(L-lysine) (PLL). To achieve effective tumor treatment, rev-caspase-3 (rev-casp-3) gene was applied to promote the constitutive apoptosis of tumor cells [24]. Negatively charged OEAL/PEI/pDNA polyplexes form at physiological pH.…”

The Recent Advances of Magnetic Nanoparticles in Medicine

“…It is another novel treatment for tumor after traditional surgery, radiotherapy, and chemotherapy [38]. But gene transfer method with safety, efficiency, and controllability is the key for gene therapy [39].…”