Hydrophobic interaction membrane chromatography for bioseparation and responsive polymer ligands involved

Design Considerations for Next‐Generation Polymer Sorbents: From Polymer Chemistry to Device Configurations

“…These short diffusion lengths along with the high porosity of the membranes present opportunities for operating at higher flow rates and reduced pressures. Adsorptive membranes have been produced in many ways (e.g., binding moieties can be incorporated onto the surface of commercially available membranes using modification schemes such as layer-by-layer deposition [33][34][35][36] as well as grafting-to [37,38] and grafting from [39,40] polymerizations), and though these approaches take advantage of enhanced mass transfer, the materials tend to have relatively low specific binding capacities. Emerging fabrication methods, which utilize self-assembled materials in conjunction with phase separation or electrospray techniques, present simple and scalable approaches to generate materials with ideal mass transfer characteristics and high specific capacities.…”

Recent development and application of membrane chromatography

“…As a low-cost material with a simple source, filter paper possesses good compatibility with biomacromolecules and good air permeability and is a suitable hydrophobic membrane matrix. Chen et al [ 12 ] successfully grafted poly(N-isopropyl acrylamide) polymer onto the surface of wood fiber of filter paper and obtained a hydrophobic interaction membrane with temperature response function, which could regulate the binding and release of target proteins through controlling the temperature of the flowing water phase. Vu et al [ 56 ] prepared hydrophobically interacting membrane adsorbents by grafting poly N-vinyl caprolactam from the surface of regenerated cellulose membrane by atom transfer radical polymerization.…”

“…1 ). Due to the advantage in mass transfer, membrane chromatography is an effective method for extracting trace proteins from large capacity feed, which can maintain their natural conformation by reducing the time in contact with adsorbents, maintaining the biological activity of the required biomolecules while removing impurities [ 11 , 12 ]. Moenster et al [ 13 ] used the SartobindQ exchange membrane adsorber method to separate and purify penicillin G amidase from cell lysate in one step, compared with previous multiple purification steps, which reduced the operating units and significantly improved the downstream processing efficiency.…”

Membrane Chromatography and Fractionation of Proteins from Whey—A Review

“…Different types of interactions between protein molecules and membrane/modified membranes are presented in Figure 2. Different types of interactions between protein molecules and membrane/modified membrane: (A) ion-exchange (Self-developed, concept was adopted from Sun et al, 2011 [93], Reif and Freitag, 1993 [94]), (B) hydrophobic interaction (Self-developed, concept was adopted from Chen et al, 2017 [95], Tennikova and Svec, 1993 [96]), (C) affinity (Self-developed, concept was adopted from Zou et al, 2001 [97], Lalli et al, 2018 [98]), and (D) mixed matrix (Self-developed, concept was adopted from Avramescu et al, 2003 [99], Saufi and Fee, 2013 [100]).…”

“…The aqueous surface tension is increased by the high concentration of salt, which promotes hydrophobic interactions instead of electrostatic interactions [100]. Butyl, octyl (alkyl), and phenyl (aryl) groups are grafted on the hydrophilic membrane matrix and used for the separation of biomolecules via hydrophobic interaction in the MC system [95]. Often, amino acids with a hydrophobic side chain, such as glycine (Gly), alanine (Ala), valine (Val), leucine (Leu), isoleucine (Ile), proline (Pro), phenylalanine (Phe), methionine (Met), and tryptophan (Trp) in the protein structure are grafted onto the membrane matrix to develop a hydrophobic membrane [113].…”