Membrane Chromatography and Fractionation of Proteins from Whey—A Review

Nath, Arijit; Zin, Moh Moh; Molnár, Máté András; Bánvölgyi, Szilvia; Gáspár, Igor; Vatai, Gyula; Kőris, András

doi:10.3390/pr10051025

Cited by 13 publications

(4 citation statements)

References 168 publications

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“…On the other hand, the 0.45 and 0.2 μm pore membranes gave rise to a prominent shift of signals from the OZ to the IZ, as the ring was suppressed, for the (+)Target samples. The reason that ring suppression occurred more significantly for the membranes with smaller pore sizes is because these substrates exhibit higher hydrophobicity, leading to slower diffusion . In addition, to observe the iFluor-RFA on the membrane according to the target nucleic acid, the surface of the 0.2 μm CN membrane of the iFluor-RFA sample was observed by scanning electron microscopy (SEM) (Figure S5).…”

Section: Resultsmentioning

confidence: 99%

“…The reason that ring suppression occurred more significantly for the membranes with smaller pore sizes is because these substrates exhibit higher hydrophobicity, leading to slower diffusion. 39 In addition, to observe the iFluor-RFA on the membrane according to the target nucleic acid, the surface of the 0.2 μm CN membrane of the iFluor-RFA sample was observed by scanning electron microscopy (SEM) (Figure S5). In samples without target DNA, accumulation of RCA products was observed only in the OZ, whereas in samples with target DNA, more accumulation was observed in the IZ.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Simple and Multiplexed Detection of Nucleic Acid Targets Based on Fluorescent Ring Patterns and Deep Learning Analysis

Lee,

Lee

et al. 2023

ACS Appl. Mater. Interfaces

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Simple diagnostic tests for nucleic acid targets can provide great advantages for applications such as rapid pathogen detection. Here, we developed a membrane assay for multiplexed detection of nucleic acid targets based on the visualization of twodimensional fluorescent ring patterns. A droplet of the assay solution is applied to a cellulose nitrate membrane, and upon radial chromatographic flow and evaporation of the solvent, fluorescent patterns appear under UV irradiation. The target nucleic acid is isothermally amplified and is immediately hybridized with fluorescent oligonucleotide probes in a one-pot reaction. We established the fluorescent ring assay integrated with isothermal amplification (iFluor-RFA = isothermal fluorescent ring-based radial flow assay), and feasibility was tested using nucleic acid targets of the receptor binding domain (RBD) and RNA-dependent RNA polymerase (RdRp) genes of SARS-CoV-2. We demonstrate that the iFluor-RFA method is capable of specific and sensitive detection in the subpicomole range, as well as multiplexed detection even in complex solutions. Furthermore, we applied deep learning analysis of the fluorescence images, showing that patterns could be classified as positive or negative and that quantitative amounts of the target could be predicted. The current technique, which is a membrane pattern-based nucleic acid assay combined with deep learning analysis, provides a novel approach in diagnostic platform development that can be versatilely applied for the rapid detection of infectious pathogens.

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Section: Resultsmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

Simple and Multiplexed Detection of Nucleic Acid Targets Based on Fluorescent Ring Patterns and Deep Learning Analysis

Lee,

Lee

et al. 2023

ACS Appl. Mater. Interfaces

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“…Membrane adsorbers (MA) are thin, micro-or macro-porous layers that are functionalized with similar ligands to those used for resin-based chromatography [177], enabling them to preserve comparable resolution in separating molecules [178][179][180]. Numerous studies have reported that ion exchange membrane adsorbers can be used to fractionate proteins from whey, but limited information is available regarding the use of affinity membrane adsorbers for whey protein separation due to the highly expensive nature of affinity chromatography [181]. The fractionation of whey proteins using such ion exchange membrane adsorbers consists of two main steps in a similar manner to resin bed chromatography, namely, anion exchange followed by cation exchange.…”

Section: Membrane Adsorber/membrane Chromatographymentioning

confidence: 99%

Separation Technologies for Whey Protein Fractionation

Chen

Gras

et al. 2023

Food Eng Rev

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Whey is a by-product of cheese, casein, and yogurt manufacture. It contains a mixture of proteins that need to be isolated and purified to fully exploit their nutritional and functional characteristics. Protein-enriched fractions and highly purified proteins derived from whey have led to the production of valuable ingredients for many important food and pharmaceutical applications. This article provides a review on the separation principles behind both the commercial and emerging techniques used for whey protein fractionation, as well as the efficacy and limitations of these techniques in isolating and purifying individual whey proteins. The fractionation of whey proteins has mainly been achieved at commercial scale using membrane filtration, resin-based chromatography, and the integration of multiple technologies (e.g., precipitation, membrane filtration, and chromatography). Electromembrane separation and membrane chromatography are two main emerging techniques that have been developed substantially in recent years. Other new techniques such as aqueous two-phase separation and magnetic fishing are also discussed, but only a limited number of studies have reported their application in whey protein fractionation. This review offers useful insights into research directions and technology screening for academic researchers and dairy processors for the production of whey protein fractions with desired nutritional and functional properties.

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“…Small-molecular-weight bioactive peptides generated using EPH have great potential as health-promoting ingredients in the form of functional foods and nutraceuticals [3]. Beyond their role as sources of nutrients, such peptides have been shown to have a wide range of biological functions, including blood glucose regulation, and antihypertensive and cholesterol-lowering effects [4,5]. In the production of such bioactive peptides for human consumption, downstream separation technologies are essential unit operations implemented to improve vital sensory attributes as well as to concentrate bioactive constituents [6].…”

Section: Introductionmentioning

confidence: 99%

Membrane Separation of Chicken Byproduct Hydrolysate for Up-Concentration of Bioactive Peptides

Dibdiakova,

Matic,

Wubshet

et al. 2024

Membranes

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Membrane processes, such as microfiltration, ultrafiltration, and nanofiltration, are increasingly used for various applications in both upstream and downstream processing. Membrane-based processes play a critical role in the field of separation/purification of biotechnological products, including protein production/purification. The possibility of using membranes to separate peptides from a chicken byproduct hydrolysate and the effect of the performed downstream processing on the DPP-IV dipeptidyl peptidase IV (DPP-IV) inhibitory activity of mechanical deboning chicken residue (MDCR) has been investigated. The chicken byproduct hydrolysate was prepared by enzymatic hydrolysis followed by microfiltration (MF), ultrafiltration (UF), nanofiltration (NF), and reverse osmosis (RO) separation. Comparing all separation treatments, hydrolysates processed only by MF and UF show the best DPP-IV inhibition (59.5–60.0% at 1 mg/mL and 34.2–40.7% at 0.5 mg/mL). These samples show dose-responsive behavior. Bioactivity was correlated with molecular weight distribution profiles and average molecular weights. The nanofiltration process notably decrease the inhibitory activity, and these permeates show low DPP-IV inhibition (9.5–21.8% at 1 mg/mL and 3.6–12.1% at 0.5 mg/mL). The size-exclusion chromatography–organic carbon detection–organic nitrogen detection (LC–OCD–OND) analysis confirms that NF and RO would retain the bioactive peptides in the concentrate in comparison to MF and UF. Bioactivity was correlated with molecular weight distribution profiles and average molecular weights. Permeates after ultrafiltration show an IC50 value of 0.75 mg/mL, comparable to other potent DPP-IV inhibitors derived from various food sources, and significantly more potent compared to the microfiltration sample, which shows an IC50 value of 1.04 mg/mL. The average molecular weight of the permeates calculated from the SEC chromatograms was 883 g/mol for UF and 1437 g/mol for MF. Of the four membranes studied, the UF membrane shows the best separation properties with respect to maximizing the yield and up-concentration of the bioactive peptides. Overall, UF was demonstrated to be a feasible technology for the removal of the undesired high-molecular-weight substances and up-concentration of small-molecular-weight bioactive peptides from chicken byproduct hydrolysate. These peptides might exhibit biological activity and could offer several health benefits. There is a high potential for the use of bioactive peptides, and more research in this field can lead to promising results that have significant effects in the food and medical industries.

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Membrane Chromatography and Fractionation of Proteins from Whey—A Review

Cited by 13 publications

References 168 publications

Simple and Multiplexed Detection of Nucleic Acid Targets Based on Fluorescent Ring Patterns and Deep Learning Analysis

Simple and Multiplexed Detection of Nucleic Acid Targets Based on Fluorescent Ring Patterns and Deep Learning Analysis

Separation Technologies for Whey Protein Fractionation

Membrane Separation of Chicken Byproduct Hydrolysate for Up-Concentration of Bioactive Peptides

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