Hydrophobic effects in reversed-phase liquid chromatography

Karger, Barry L.; Gant, J.R.; Martkopf, Arleigh; Weiner, Paul H.

doi:10.1016/s0021-9673(00)84032-7

Cited by 410 publications

(105 citation statements)

References 31 publications

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“…Analysis and prediction of chromatographic separations are usually carried out based on the linCorrespondence concerning this article should be addressed to K. Miyabe ear relations. Many experimental data concerning the linear relations have been reported and suggest different conclusions relating to the linearity in the range of capacity factor less than unity and the influence of the kind of solvent on the linearity (Berendsen and de Galan, 1980;Hennion et al, 1978;Karger et al, 1976;Schoenmakers et al, 1979).…”

Section: Introductionmentioning

confidence: 99%

Solvent effect on adsorption phenomena in reversed‐phase liquid chromatography

Miyabe

Suzuki

1995

AIChE Journal

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Section: Introductionmentioning

confidence: 99%

Solvent effect on adsorption phenomena in reversed‐phase liquid chromatography

Miyabe

Suzuki

1995

AIChE Journal

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“…In order words, in reverse phase chromatography, molecular interactions between the solutes and stationary phase ("solvophobic interactions") play an important part in separations (Ref. [51][52]. · Such experimental results bring contribution usefully to a best understanding of the nature of the different structural and molecular parameters involved in chromatographic retentions by such grafted phases but they have up to now poor theoretical bases, clearing the different mechanisms involved in LLC Separations.…”

Section: ~ ~)mentioning

confidence: 99%

Theoretical and experimental aspects of fractionation mechanisms in liquid chromatography on macromolecular stationary phases (gel or bonded chains)

Lecourtier¹,

Audebert²,

Quivoron³

1979

Pure and Applied Chemistry

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Abstract-In a preliminary part, we deal with the partition mechanism of low or high molecular weight solutes, by selective dissolution in swollen gels. After summing up the theoretical expressions, we present some experimental evidences for a fractionation mechanism based on such a phenomenon, which may interfere , in many cases, with the classical steric exclusion effect in GPC. Most of this paper is devoted to chemically bonded stationary phases which gained increasing importance in H.P.L.C., during the past few years. Owing to our previous works on gels, we theoretically studied the partition coefficient of low or high molecular weight solutes between a mobile phase and a stationary phase, constituted by macromolecular chains bonded to an inert surface. Variations of partition coefficient with the molecular sizes of solute, solvent and graft, and with the molecular interaction parameters between these species, are presented. In a complementary aspect, we describe the recent preparation of model chemically bonded phases of silica/polyethyleneoxide and silica/polystyrene types. The retention data obtained for various solutes on these bonded phases are discussed in terms of silica loading, grafting chain length, solute size and solvent nature. These theoretical and experimental studies lead to practical consequences concerning the relative parts of the dissolution effect into the grafted phase, the adsorption on the mineral support and the steric exclusion effect due to the residual porosity.

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“…It is also insensitive to impurities and needs a very small amount of sample in comparison to the time-consuming and tedious shake flask or slow-stirring methods [13,14]. Estimation of the partition coefficient is based on the retention factor (log K) [15][16][17][18]. Hydrophobic reversed-based silica-based stationary phases are most frequently used for the determination of lipophilicity [19].…”

Section: Introductionmentioning

confidence: 99%

Determination of Octanol-Water Partition Coefficient of Novel Coumarin Based Anticancer Compounds by Reversed-Phase Ultra-Fast Liquid Chromatography

Sairam

Gurupadayya

Iyer

et al. 2017

Int J Pharm Pharm Sci

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Objective: The present study aims at the development of a reversed phase ultra-fast liquid chromatography (RP-UFLC) method for measurement of the lipophilicity (log P) between n-octanol and water for the newly synthesized coumarin derivatives in our laboratory. Methods:The synthesized compounds were dissolved in methanol and analyzed using XTerra RP18 column as the stationary phase and a mixture of methanol (0.25% v/v octanol) and buffer as the mobile phase with isocratic elution. Results:In this study we concentrated on the relationship between a reversed-phase ultra-fast liquid chromatography (RP-UFLC) retention parameters and log P of our synthesized compounds. Furthermore, a good correlation and very close values were obtained between the experimentally determined log P values and values obtained from Chemdraw. Conclusion:The developed method was found to be insensitive to any of the impurities present and moreover it requires very little sample for analysis.

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Hydrophobic effects in reversed-phase liquid chromatography

Cited by 410 publications

References 31 publications

Solvent effect on adsorption phenomena in reversed‐phase liquid chromatography

Solvent effect on adsorption phenomena in reversed‐phase liquid chromatography

Theoretical and experimental aspects of fractionation mechanisms in liquid chromatography on macromolecular stationary phases (gel or bonded chains)

Determination of Octanol-Water Partition Coefficient of Novel Coumarin Based Anticancer Compounds by Reversed-Phase Ultra-Fast Liquid Chromatography

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