Determination of Octanol-Water Partition Coefficient of Novel Coumarin Based Anticancer Compounds by Reversed-Phase Ultra-Fast Liquid Chromatography

Abstract

Objective: The present study aims at the development of a reversed phase ultra-fast liquid chromatography (RP-UFLC) method for measurement of the lipophilicity (log P) between n-octanol and water for the newly synthesized coumarin derivatives in our laboratory. Methods:The synthesized compounds were dissolved in methanol and analyzed using XTerra RP18 column as the stationary phase and a mixture of methanol (0.25% v/v octanol) and buffer as the mobile phase with isocratic elution. Results:In this study we concentrated on the relationship between a reversed-phase ultra-fast liquid chromatography (RP-UFLC) retention parameters and log P of our synthesized compounds. Furthermore, a good correlation and very close values were obtained between the experimentally determined log P values and values obtained from Chemdraw. Conclusion:The developed method was found to be insensitive to any of the impurities present and moreover it requires very little sample for analysis.