Hydrophilic-interaction chromatography of complex carbohydrates

Alpert, Andrew J.; Shukla, Mukta; Shukla, Ashok; Zieske, Lynn R.; Yuen, Sylvia; Ferguson, Michael A. J.; Mehlert, Angela; Pauly, Markus; Orlando, Ron

doi:10.1016/0021-9673(94)00467-6

Cited by 232 publications

(125 citation statements)

References 34 publications

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“…The increase of temperature, pH and acetonitrile concentration have been shown to facilitate the collapse of anomer peaks, resulting in one distinctive single peak. It causes also about three times lower detection limits than by the initial conditions [25].…”

Section: Amide Stationary Phasesmentioning

confidence: 91%

Application of hydrophilic interaction chromatography in phytochemical analysis

Petruczynik¹,

Waksmundzka‐Hajnos²

2013

Acta Chromatographica

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Summary. Hydrophilic interaction chromatography (HILIC) is a liquid chromatographytechnique when a polar stationary phases -silica or polar bonded phases and aqueous mobile phase containing amount of a less polar solvent (often acetonitrile) is applied. HILIC is important for the separation of highly polar substances including biologically active compounds, such as drugs, amino acids, peptides, proteins, nucleosides, neurotransmitters, etc. HILIC is also the appropriate method for analysis of plant extract polar components such as carbohydrates, amino acids, peptides, phenolic acids, flavonoids and some alkaloids. Plant extracts are usually multicomponent mixtures of compounds of wide polarity range, which often cannot be analyzed by use of a single separation and detection method because of the high chemical diversity. Good results are obtained by use of coupled method, e.g., reversed-phase liquid chromatography (RP-LC) and HILIC mode separation. The elution order in HILIC is usually opposite to that in RP separations. This orthogonality determines one of the advantages of HILIC -the possibility of creating multidimensional separations. An important feature of HILIC is the improved sensitivity with electrospray mass spectrometry. This is significant for the analysis of components existing in small concentration in multicomponent mixtures. The high acetonitrile content also gives additional advantage of HILIC -faster separations due to the lower viscosity of HILIC eluents compared to standard RP ones. The presented review deals with the optimization of HILIC separations and application of the method for analysis of plant extracts components.

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Section: Amide Stationary Phasesmentioning

confidence: 91%

Application of hydrophilic interaction chromatography in phytochemical analysis

Petruczynik¹,

Waksmundzka‐Hajnos²

2013

Acta Chromatographica

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“…Kimura et al reported a method that used hydrophilic interaction liquid chromatography (HILIC) coupled with an evaporative light-scattering detector (ELSD) (Kimura et al, 2004); This method is known as the HILIC-ELSD method. HILIC has been developed as an efficient tool for analyzing highly hydrophilic compounds, and its analytical application to carbohydrates (Alpert et al, 1994) and peptides (Alpert et al, 1990) has been reported. The retention ability of the HILIC column basically depends on the hydrophilicity of the analytes, and if the compound has amino groups, its retention time is increased (Tolstikov & Fiehn, 2002).…”

Section: Methods For Determining Dnj In Mulberrymentioning

confidence: 99%

Development of Mulberry Leaf Extract for Suppressing Postprandial Blood Glucose Elevation

Kimura¹

2011

Hypoglycemia - Causes and Occurrences

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“…PolyGlycoplex columns are suitable for separations of monosaccharides, oligosaccharides, and sialyl sugars or their p-nitrobenzyloxy derivatives (Alpert et al, 1994).…”

Section: Chapter 2 Advances In Hydrophilic Interaction Liquid Chromatmentioning

confidence: 99%