Hydroperoxide production from linoleic acid by heterologous Gaeumannomyces graminis tritici lipoxygenase: Optimization and scale-up

Villaverde, Juan José; Vlist, Vincent van der; Santos, Sónia A.O.; Haarmann, Thomas; Langfelder, Kim; Pirttimaa, Minni; Nyyssölä, Antti; Jylhä, Sirpa; Tamminen, Tarja; Kruus, Kristiina; Graaff, Leo de; Neto, Carlos Pascoal; Simões, Mário M. Q.; Domingues, Rosário M.; Silvestre, Armando J. D.; Eidner, Jasmin; Büchert, Johanna

doi:10.1016/j.cej.2012.11.090

Cited by 28 publications

(28 citation statements)

References 30 publications

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“…Wang et al [8] suggested that lignin can be used as a template for the synthesis of porous carbon-CeO 2 composites, and Vilela et al [9] carried out the first investigation on the use of lipophilic extracts from the ripe pulp of ten banana cultivars. In addition, vegetable oils from biomass residues have been widely used as renewable raw materials in many applications, including adhesives, paints, coatings, plasticizer, detergents, and lubricants [10].…”

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confidence: 99%

Analytical Pyrolysis Study of Peanut Shells using TG-MS Technique and Characterization for the Waste Peanut Shell Ash

Yao¹,

Xu²,

Liang³

2016

JRST

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ABSTRACT:As an agricultural waste produced from the processing of peanut, the yield of peanut shells in China is quite abundant. Although numerous studies on the pyrolysis of peanut shells have been carried out, most of them were focused on the dynamics models for predicting kinetics parameter of biomass, and rarely on the evolution of gaseous products in pyrolysis. This study carried out a set of experiments on the pyrolysis of peanut shells under different heating rates in order to fill the knowledge gap. Besides, there is also a lack of information available concerning the characterization of resulting peanut shell ash. Thus, the aim of this study is to investigate the pyrolysis behaviors of peanut shells thoroughly and to characterize the properties of peanut shell ash that serves as predictors for its applications. The results show that the decomposition of peanut shells follows a stepwise mechanism, and the activation energy calculated by FlynnWall-Ozawa method varies from 58.3 to 88.6 kJ·mol -1 . Compared with low heating rate, fast heating rate at low temperatures is more suitable for biogas production. Chemical and phase analysis shows that peanut shell ash has a high SiO 2 content except for other oxides (K 2 O, CaO, MgO, Al 2 O 3 , etc.) that quite suitable in glass production, implying its potential for glass production and as a mineral admixture in concrete. Released volatile matters in pyrolysis create many pores in carbonaceous materials, thus low-cost adsorbents can be produced from these carbon residues.

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confidence: 99%

Analytical Pyrolysis Study of Peanut Shells using TG-MS Technique and Characterization for the Waste Peanut Shell Ash

Yao¹,

Xu²,

Liang³

2016

JRST

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“…Production of hydroperoxides by LOX has been investigated on laboratory scale by Villaverde et al They have described the synthesis of 13-hydroperoxy-(9Z,11E)-octodecadienoic acid from linoleic acid by means of two lipooxygenases from Gaeumannomyces graminis [92] and Pseudomonas aeruginosa [93]. Both biotransformations resulted in high yield (88% and 75%) and selectivity (74% and 61%) of the desired product.…”

Section: Methodsmentioning

confidence: 98%

“…The new conditions caused a yield decrease of 40% leading to a volumetric productivity of 3.6 g L -1 h -1 . The authors of this research suggested that the process could likely be improved by facilitating the solubility of the fatty acid as well as oxygen supply to the reaction medium [92].…”

Section: Methodsmentioning

confidence: 99%

Engineering and application of enzymes for lipid modification, an update

Zorn

Oroz‐Guinea

Brundiek³

et al. 2016

Progress in Lipid Research

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This review first provides a brief introduction into the most important tools and strategies for protein engineering (i.e. directed evolution and rational protein design combined with high-throughput screening methods) followed by examples from literature, in which enzymes have been optimized for biocatalytic applications. This covers engineered lipases with altered fatty acid chain length selectivity, fatty acid specificity and improved performance in esterification reactions. Furthermore, recent achievements reported for phospholipases, lipoxygenases, P450 monooxygenases, decarboxylating enzymes, fatty acid hydratases and the use of enzymes in cascade reactions are treated.

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“…Hydroperoxide production and its further chemical reactions Villaverde et al, 2013a;Villaverde et al, 2013b;Hayes, 2004;Maurer & Schmid, 2005 Removal of wood extractives in mechanical pulping…”

Section: Gaeumannomyces Graminis Glycine Max Pseudomonas Aeruginosamentioning

confidence: 99%

Industrial potential of lipoxygenases

Heshof

Graaff

Villaverde

et al. 2015

Critical Reviews in Biotechnology

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Lipoxygenases (LOXs) are iron- or manganese-containing oxidative enzymes found in plants, animals, bacteria and fungi. LOXs catalyze the oxidation of polyunsaturated fatty acids to the corresponding highly reactive hydroperoxides. Production of hydroperoxides by LOX can be exploited in different applications such as in bleaching of colored components, modification of lipids originating from different raw materials, production of lipid derived chemicals and production of aroma compounds. Most application research has been carried out using soybean LOX, but currently the use of microbial LOXs has also been reported. Development of LOX composition with high activity by heterologous expression in suitable production hosts would enable full exploitation of the potential of LOX derived reactions in different applications. Here, we review the biological role of LOXs, their heterologous production, as well as potential use in different applications. LOXs may fulfill an important role in the design of processes that are far more environmental friendly than currently used chemical reactions. Difficulties in screening for the optimal enzymes and producing LOX enzymes in sufficient amounts prevent large-scale application so far. With this review, we summarize current knowledge of LOX enzymes and the way in which they can be produced and applied.

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Hydroperoxide production from linoleic acid by heterologous Gaeumannomyces graminis tritici lipoxygenase: Optimization and scale-up

Cited by 28 publications

References 30 publications

Analytical Pyrolysis Study of Peanut Shells using TG-MS Technique and Characterization for the Waste Peanut Shell Ash

Analytical Pyrolysis Study of Peanut Shells using TG-MS Technique and Characterization for the Waste Peanut Shell Ash

Engineering and application of enzymes for lipid modification, an update

Industrial potential of lipoxygenases

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