Hydropericarditis syndrome in broiler chickens in Pakistan

Anjum, A. D.; Sabri, Mustafa; Iqbal, Zeeshan

doi:10.1136/vr.124.10.247

Cited by 119 publications

(80 citation statements)

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“…The consistent and predominant gross lesion at necropsy of affected birds is hydropericardium (Anjum et al, 1989). The liver is friable, mottled, swollen and discolored had multiple regions of focal necrosis, pin point or ecchymotic hemorrhages.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Evaluation of Lyophilized Live Attenuated Vaccine of Inclusion Body Hepatitis Hydropericardium Syndrome (IBH-HPS) against Challenge in Broiler Chickens

Ali¹,

Mahmood²,

Hussaın³

et al. 2015

IJAB

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The current study was conducted to prepare and evaluate the efficacy of live attenuated vaccine of IBH-HPS in broiler birds in Pakistan. The IBH-HPS virus was successfully adapted after three blind passages (Vero cell line) and the 4 th passage was fully adapted as it gave cytopathogenic effect (CPE) in 48 h post infection in Vero cells. The 9 th passage was determined for attenuation in broiler birds. The attenuated virus was subjected to pathogenecity testing and used as vaccine candidate in broiler chickens. Two hundred and fifty, day-old commercial broiler chicks were purchased from local market, divided into 5 groups and offered feed and water ad libitum. At the age of 15 days, 50 birds in each group were administered with 9 th passage live attenuated lyophilized vaccine, the 3 rd passage ß-propiolactone inactivated vaccine (prepared in the laboratory), oil emulsified inactivated vaccine (UAF-Angavac), commercial vaccine (Bio-Angara, Sana Laboratories, Pakistan), and normal saline, respectively as negative control. The 9 th passage live attenuated lyophilized vaccine protected 100% birds from mortality, clinical signs and high antibody titre was observed. On the other hand, ß-propiolactone inactivated vaccine gave 70% protection. The oil emulsified inactivated vaccine gave 80% protection but lower weight gain was observed. The commercial vaccine gave 60% protection. In negative control 70% mortality was recorded. The results revealed that the live attenuated lyophilized vaccine showed a higher protection against lethal challenge of IBH-HPS in broilers.

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Section: Introductionmentioning

confidence: 99%

Preparation and Evaluation of Lyophilized Live Attenuated Vaccine of Inclusion Body Hepatitis Hydropericardium Syndrome (IBH-HPS) against Challenge in Broiler Chickens

Ali¹,

Mahmood²,

Hussaın³

et al. 2015

IJAB

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“…The ® rst occurrence of infectious hydropericardium (IHP) was reported in Angara Goth in Pakistan in late 1987 (Anjum et al, 1989). The disease is also called Angara Disease or Hepatitis/ Hydropericardium Syndrome.…”

Section: Introductionmentioning

confidence: 99%

Epidemiological studies on fowl adenoviruses isolated from cases of infectious hydropericardium

1999

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Serology, restriction enzyme analysis and polymerase chain reactions were used to classify a total of 12 fowl adenoviruses (FAV) isolated from clinical cases of infectious hydropericardium from ® eld outbreaks in seven countries in Asia and America. All isolates belonged to FAV serotype 4. Two isolates were contaminated with avian adeno-associated virus and one of them also contained FAV1. Minor differences were observed in the BamHI restriction pro® les. More variability was seen with SmaI, BglII and PstI restriction pro® les. However, more than 80% of the fragments were identical in size in the ® ve different PstI pro® les, indicating the close genomic relationship between the isolates. Polymerase chain reaction assays supported the classi® cation of the isolates as FAV4 strains. All isolates could be detected using H1/H2 or H3/H4 primer pairs. Restriction enzyme analysis of the H1/H2 polymerase chain reaction (PCR) products allowed no differentiation between the isolates, whereas the three isolates from India and Pakistan could be separated from all others after HpaII digestion of the H3/H4 PCR products. Although strain variation was demonstrated, it could be shown that all adenoviruses isolated from various ® eld cases of infectious hydropericardium (Angara Disease) in several countries belong to fowl adenovirus serotype 4.

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“…IBH is usually seen in meat-producing birds between three and seven weeks of age. Morbidity is low and Mortality usually ranges between 5% and 10%, but it can reach 30 % (12)(13)(14). The main postmortem finding is pale, friable and swollen liver with multiple petechial hemorrhage (15)(16)(17).…”

Section: Introductionmentioning

confidence: 99%

Molecular Identification of Fowl Adenovirus Associated with Inclusion Body Hepatitis in Iran

Hosseini¹,

Morshed²

2012

Iran J Virol

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Background and Aims: Inclusion body hepatitis (IBH) is one of the most important reemerging diseases in many countries with intensive poultry industries. In Iran, the etiological agent of IBH (fowl adenovirus) has not been yet confirmed. The aim of this study was the molecular detection and identification of fowl adenovirus involving IBH in chicken flocks in Iran. Materials and Methods: Polymerase chain reaction (PCR) and sequence analysis of L1 hexon gene was utilized to detect and to determinate the genotypes of Fowl adenovirus (FAdV) in broiler breeder flocks. Histopathological sections were prepared and examined. Results: FAdVs were detected in livers. Based on sequencing analysis of the hexon gene, they were genetically related to FAdV-11, a member of the fowl adenovirus D species, with 98% homology to Korean strain in 2011. Histological examination revealed necrotizing hepatitis with basophilic intranuclear inclusion bodies in the hepatocytes. Conclusion: This study provided evidence for the role of fowl adenoviruses as agents, causing this clinical disease in Iran and indicated the importance of accurate diagnosis and prevention in the meat-type flocks.

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Hydropericarditis syndrome in broiler chickens in Pakistan

Cited by 119 publications

References 0 publications

Preparation and Evaluation of Lyophilized Live Attenuated Vaccine of Inclusion Body Hepatitis Hydropericardium Syndrome (IBH-HPS) against Challenge in Broiler Chickens

Preparation and Evaluation of Lyophilized Live Attenuated Vaccine of Inclusion Body Hepatitis Hydropericardium Syndrome (IBH-HPS) against Challenge in Broiler Chickens

Epidemiological studies on fowl adenoviruses isolated from cases of infectious hydropericardium

Molecular Identification of Fowl Adenovirus Associated with Inclusion Body Hepatitis in Iran

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