Hydrolytic enzyme production from açai palm (Euterpe precatoria) endophytic fungi and characterization of the amylolytic and cellulolytic extracts

Batista, Bárbara Nunes; Matias, Rosiane Rodrigues; Oliveira, Rafael Lopes e; Albuquerque, Patrícia Melchionna

doi:10.1007/s11274-021-03217-w

Cited by 14 publications

(9 citation statements)

References 47 publications

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“…Lipolytic activity was evaluated according to the formation (or not) of a clear halo around the colony, which was measured with a caliper. The EI was calculated by the ratio between the diameter of the halo and the diameter of the colony (Batista et al, 2022). Cultivation in the solid medium was carried out in triplicate.…”

Section: Enzymatic Evaluation In Solid Mediamentioning

confidence: 99%

“…This enzymatic activity was significantly different from the lipase production observed for the other fungi (Figure 1). Interestingly, if only the solid medium assay were to be considered, this fungus would not be selected, since usually only microorganisms with EI ≥2 are considered potential producers of enzymes (Batista et al, 2022). Source: Authors.…”

Section: Submerged Fermentationmentioning

confidence: 99%

“…HEs have vast catalytic versatility and represent important industrial inputs that comprise a wide range of applications (laundry detergent, textiles, cellulose and paper, leather, cosmetics, biofuel and food industries). Of all the HEs, amylases, cellulases, xylanases, pectinases, proteases and lipases are among the most industrially used (Krishnan et al, 2016;Thapa et al, 2019;Batista et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

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Lipase production from Aniba canelilla endophytic fungi, characterization and application of the enzymatic extract

Matias

Rodrigues²,

Procópio³

et al. 2022

RSD

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Endophytic fungi (EF) have a notable capacity to produce active molecules of industrial importance, such as hydrolytic enzymes. In this study we investigated the production of lipase by EFs isolated from the Amazonian species Aniba canelilla (Lauraceae), characterized the enzymatic extract obtained from the most promising fungus, and applied the lipolytic extract as a biocatalyst in the transesterification reaction for biodiesel production. The fungi were submitted to enzymatic screening in solid medium and in submerged fermentation to assess their lipase production. A total of 292 fungi were tested in solid media. Lipolytic activity was detected in 74% of the fungi cultivated in liquid media, 18 of which showing promising enzymatic production. The best lipase producer, Endomelanconiopsis endophytica QAT_7AC, was identified by sequencing of the ITS region. After adjusting the bioprocess conditions, E. endophytica QAT_7AC produced 2,415.5 U/mL of lipase after 72 h. The enzymatic extract showed higher lipolytic activity under pH 8.0 and 40 oC. The extract was applied as a biocatalyst in a transesterification reaction performed at 40 oC, with ethanol and waste cooking oil (3:1). The biodiesel yield was found to be 87% after 2 h rection when the fungal enzyme was used and 89% with the commercial biocatalyst. The endophytic fungi isolated from A. canelilla proved themselves to be biotechnologically relevant, as they can be explored as potential producers of lipases. The lipolytic extract can be applied in the synthesis of biodiesel using waste cooking oil.

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Section: Enzymatic Evaluation In Solid Mediamentioning

confidence: 99%

Section: Submerged Fermentationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Lipase production from Aniba canelilla endophytic fungi, characterization and application of the enzymatic extract

Matias

Rodrigues²,

Procópio³

et al. 2022

RSD

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“…Nodilusporium. 11 Molds can be associated with plants, animals, and other microorganisms. Several studies have successfully found various types of mold from various substrates such as animal feed, 10 orange leaves (Citrus nobilis), 12 and apples.…”

Section: Introductionmentioning

confidence: 99%

“…15 Growth conditions such as pH and temperature of the medium can affect the amylase activity. 11 In this study, molecular identification of molds isolated from August flower (P. venusta) were performed, followed by culture-dependent analysis. The isolates were then tested for their ability to produce amylases and obtained the optimal temperature and pH of growth using the Solid State Fermentation (SSF) method.…”

Section: Introductionmentioning

confidence: 99%

Production of Amylase by Aspergillus subflavus and Aspergillus fumigatus from flamevine flower (Pyrostegia venusta (Ker-Gawl.) Miers): A Tropical Plant in Bedugul Botanical Garden, Bali, Indonesia

Sukmawati¹,

Dellanerra²,

Fikriyyah³

et al. 2022

J. Pure Appl. Microbiol.

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Pyrostegia venusta is known as an ornamental plant with its source of antioxidants, cytotoxic, anti-inflammatory, and anti-HIV compounds. Ephypitic molds are potentially co-existed on the surface of this flower since it contains essential nutrients which support their growth. On the other hand, molds produce several enzymes that might involve flower growth. The presence of ephypitic molds on this flower provides information about its ability to produce amylase. This study successfully isolated molds from August flower (P. venusta) originating from Taman Nasional Bedugul, Bali, Indonesia. The study aimed to isolate potential amylase producer strains and optimize the enzyme production using Solid-State Fermentation (SSF) method. Ten mold isolates belonging to Universitas Negeri Jakarta Culture Collection (UNJCC) were selected according to their amylolytic index (IA) values, morphological identification, and colony count number. Selected strains were optimized for its growth to produce amylase using the SSF method under different temperatures (30, 40, 50°C) and pH (6, 7, 8) with a wheat brain fermentation medium. Results showed that UNJCC F100 (6.53 × 108 CFU/ml) and UNJCC F106 (9.83 x 108 CFU/ml) are the two isolates with the highest IA values of 1.34 ± 0.1 and 1.08 ± 0.12 among all isolates. Based on molecular identification using ITS region, UNJCC F100 and UNJCC F106 were identified as A. subflavus (97% homology) and A. fumigatus (99.52% homology), respectively. This study exhibited that both isolate UNJCC F100 and isolate UNJCC F106 have optimal amylase production conditions at 30°C and pH 6. The enzyme produced was 19.99 U/ml at 30°C and 34.33 U/ml at pH 6 for isolate UNJCC F100, and for isolate UNJCC F106 is 28.55±3.80 U/ml. The two isolates are potentially used for amylase production, referring to the specific environmental condition. However, to generate a higher amount with amylase activity, other external variables such as medium used, inoculum concentration, and fermentation method are important to consider further for a larger application.

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Recent Advances in Pharmaceutically Important Compounds from Endophytic Fungi

Takahashi,

Marques,

Ferreira

et al. 2024

Fungi Bioactive Metabolites

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Hydrolytic enzyme production from açai palm (Euterpe precatoria) endophytic fungi and characterization of the amylolytic and cellulolytic extracts

Cited by 14 publications

References 47 publications

Lipase production from Aniba canelilla endophytic fungi, characterization and application of the enzymatic extract

Lipase production from Aniba canelilla endophytic fungi, characterization and application of the enzymatic extract

Production of Amylase by Aspergillus subflavus and Aspergillus fumigatus from flamevine flower (Pyrostegia venusta (Ker-Gawl.) Miers): A Tropical Plant in Bedugul Botanical Garden, Bali, Indonesia

Recent Advances in Pharmaceutically Important Compounds from Endophytic Fungi

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