Hydrolysis of wheat flour arabinoxylan, acid-debranched wheat flour arabinoxylan and arabino-xylo-oligosaccharides by β-xylanase, α-l-arabinofuranosidase and β-xylosidase

Soluble Dietary Fiber Reduces Trimethylamine Metabolism via Gut Microbiota and Co‐Regulates Host AMPK Pathways

“…These variations in the specific polysaccharide content are likely to cause differences in microbial community profiles and activity of microbial SDF‐degrading enzymes. For example, degradation of these specific polysaccharides by the gut microbiota involves the production of specific enzymes, such as xylanases, arabinoxylanases, and β‐glucanases . In addition, these pentosans differ in molecular weight and substitution degree (arabinose/xylose), which may also exert different modulatory effects on gut microbiota .…”

GH62 arabinofuranosidases: Structure, function and applications

“…Some ABFs specifically release both 1,2-and 1,3-linked from singly substituted Xylp, which are given the suffix m2,3 (Lange et al, 2006;McCleary et al, 2015;Siguier et al, 2014;Van Laere et al, 1999;Wilkens et al, 2016). ABFs that release Araf from doubly substituted Xylp are specifically releasing either 1,2-or 1,3-linked given the surfix d2 or d3, respectively (Cartmell et al, 2011;McKee et al, 2012;Pouvreau et al, 2011;Van Laere et al, 1997.…”

“…Nine of the 11 GH62 ABFs tested on both L-arabinan and AX showed from 9 (Couturier et al, 2011;Siguier et al, 2014) to 10 4 fold (McCleary et al 2015) lower specific activity on L-arabinan than AXs (Table 1). While the catalytic efficiency of SthAbf62A-m2,3 and AnAbf62A-m2,3 was 75 and 520 fold lower, respectively, on L-arabinan than WAXs (Tsujibo et al, 2002;Wang et al, 2014;Wilkens et al, 2016), both PaAbf62A and UmAbf62A had higher kcat on sugar beet Larabinan than on WAX (Table 1) (Siguier et al, 2014).…”

A Synthetic Glycan Microarray Enables Epitope Mapping of Plant Cell Wall Glycan-Directed Antibodies

“…Thus, the secondary cell walls in the stele contain xylans with a low degree of arabinosylation, whereas the primary walls in cortex cells contain only highly arabinosylated xylans (Christensen et al 2010). To confirm that the binding of CCRC-M154 to the cortex cells results from their high arabinoxylan content, we applied arabinoxylan-specific arabinofuranosidases (McCleary et al 2015) to the sections prior to antibody staining. Indeed, CCRC-M154 binding was completely abolished in all cell walls, while CCRC-M148 now also bound to the cortex cell walls in addition to the stele walls.…”