Soluble Dietary Fiber Reduces Trimethylamine Metabolism via Gut Microbiota and Co‐Regulates Host AMPK Pathways

Li, Qian; Wu, Tao; Liu, Rui; Zhang, Min; Wang, Ruijun

doi:10.1002/mnfr.201700473

Cited by 54 publications

(50 citation statements)

References 50 publications

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“…Interestingly, weaker intestinal epithelial AMPK β expression levels indicated by low fluorescence intensity observed in the immunoblot image were observed in the red meat diet group compared to those in the fiber-rich diet groups (Figure 5c). This is consistent with our previous observation that a diet rich in red meat can inhibit AMPK expression levels (Li et al, 2017). The distance-based redundancy analysis (RDA) ordination plots illustrated the relationship between the differences observed in the CAZyme gene family profile of gut microbiota genomes with environmental variable (Figure 5d).…”

Section: Analysis Of Gene Family Profiles From the Cazy Database Ofsupporting

confidence: 90%

“…DF was extracted from fermented wheat bran prepared by inoculation with Rhizopus oryzae (obtained from biology laboratory in Tianjin University of Science and Technology) as described previously (Li, Wu, Liu, Zhang, & Wang, 2017). DF was extracted from fermented wheat bran prepared by inoculation with Rhizopus oryzae (obtained from biology laboratory in Tianjin University of Science and Technology) as described previously (Li, Wu, Liu, Zhang, & Wang, 2017).…”

Section: Methodsmentioning

confidence: 99%

“…(Shandong Province, China). DF was extracted from fermented wheat bran prepared by inoculation with Rhizopus oryzae (obtained from biology laboratory in Tianjin University of Science and Technology) as described previously (Li, Wu, Liu, Zhang, & Wang, 2017). Commercial chemicals were of the highest purity available and were purchased from Sigma or Sigma-Aldrich unless otherwise noted.…”

Section: Methodsmentioning

confidence: 99%

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Potential correlation between carbohydrate‐active enzyme family 48 expressed by gut microbiota and the expression of intestinal epithelial AMP‐activated protein kinase β

Zhang

et al. 2019

J Food Biochem

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The expression of the carbohydrate‐active enzyme family and related genes is known to be influenced by the response of intestinal microbiota to dietary changes. However, it is uncertain whether this is caused by variation in the intestinal microecology. In this study, metabolite analysis, 16S rDNA sequencing, metagenomics, and Western blotting were employed to investigate the effects of dietary intervention on the composition of gut microbiota and microbiota‐mediated changes. The results showed that compared with the low fiber‐fed group, the fiber diet‐fed mice displayed a shift in gut microbiota composition to contain more members of phylum Bacteroidetes, accompanied by higher proportions of Akkermansia and typical probiotic Bifidobacterium. Moreover, correlations were found between microbial genes coding for carbohydrate‐binding module family 48 (CBM48) and intestinal epithelial expression levels of AMPK β. This finding provides new insight for elucidating the contribution of dietary intervention through AMPK regulation linked to the microbial carbohydrate‐binding family. Practical applications The relationship suggested by these data will provide theoretical and applied foundations for the development of potential intervention targeting the interaction between gut microbiota and host health, particularly the use of dietary fiber as a medically relevant food. Additionally, a better understanding of the interactions between gut microbiota and intestinal epithelial will inform the development of gut microbiota intervention as a health‐promoting procedure.

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Section: Analysis Of Gene Family Profiles From the Cazy Database Ofsupporting

confidence: 90%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Potential correlation between carbohydrate‐active enzyme family 48 expressed by gut microbiota and the expression of intestinal epithelial AMP‐activated protein kinase β

Zhang

et al. 2019

J Food Biochem

Self Cite

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“…Red meat consumption increased systemic TMAO levels, but high versus low levels of saturated fat in the context of a red meat diet did not affect plasma TMAO or TMAO precursors [24]. Li et al (2017) investigated the effect of soluble dietary fiber on TMA metabolism in mice fed red meat and found that soluble dietary fibers reduced TMA and TMAO metabolism and modified the gut microbiota in mice fed with red meat [25]. Background diets in the present study were characterized by a complex composition and varied in several parameters.…”

Section: Background Diet Affects Urinary Tmao Excretion Following Redmentioning

confidence: 91%

“…Thus, the high content of fruits and vegetables in the prudent background diet might contribute to the attenuation of TMAO formation. Likewise, the higher fiber content in the prudent background diet may lead to a reduced TMAO formation [25]. Only a few studies have investigated the effect of red versus white meat consumption on urinary TMAO excretion, and urinary TMAO levels do not necessarily correspond to plasma TMAO levels as shown, for example, by Bielinska et al (2018) [34] and Jakobsen et al (2017) [15].…”

Section: Background Diet Affects Urinary Tmao Excretion Following Redmentioning

confidence: 99%

Background Diet Influences TMAO Concentrations Associated with Red Meat Intake without Influencing Apparent Hepatic TMAO-Related Activity in a Porcine Model

et al. 2020

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Red meat has been associated with an increased cardiovascular disease (CVD) risk, possibly through gut microbial-derived trimethylamine-N-oxide (TMAO). However, previous reports are conflicting, and influences from the background diet may modulate the impact of meat consumption. This study investigated the effect of red and white meat intake combined with two different background diets on urinary TMAO concentration and its association with the colon microbiome in addition to apparent hepatic TMAO-related activity. For 4 weeks, 32 pigs were fed chicken or red and processed meat combined with a prudent or western background diet. 1 H NMR-based metabolomics analysis was conducted on urine samples and hepatic Mrna expression of TMAO-related genes determined. Lower urinary TMAO concentrations were observed after intake of red and processed meat when consumed with a prudent compared to a western background diet. In addition, correlation analyses between urinary TMAO concentrations and relative abundance of colon bacterial groups suggested an association between TMAO and specific bacterial taxa. Diet did not affect the hepatic Mrna expression of genes related to TMAO formation. The results suggest that meat-induced TMAO formation is regulated by mechanisms other than alterations at the hepatic gene expression level, possibly involving modulations of the gut microbiota.

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Inulin does not affect trimethylamine N‐oxide formation in mice with a high‐fat diet combined with choline and L‐carnitine

Wang,

Hu,

et al. 2024

Food Science & Nutrition

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Emerging evidence suggests that gut bacteria‐derived trimethylamine N‐oxide (TMAO) elevates the risk of cardiovascular disease, and dietary fiber holds the potential to attenuate TMAO formation. However, the effectiveness of dietary fiber, such as inulin, in inhibiting TMAO formation remains controversial. Therefore, this study investigated the effect of inulin supplementation on TMAO formation in mice with high TMAO levels induced by a high‐fat diet (HFD) combined with choline and L‐carnitine. Results showed that HFD treatment significantly elevated blood TMAO concentrations and increased the abundances of TMAO formation‐associated gut bacteria, as well as the abundances of functional genes responsible for TMA formation. While the supplementation of choline and L‐carnitine greatly enhanced blood trimethylamine (TMA) and TMAO levels, inulin supplementation did not significantly affect TMAO levels and had limited impact on TMA‐associated gut bacteria, except for Desulfitobacterium hafniense.

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Soluble Dietary Fiber Reduces Trimethylamine Metabolism via Gut Microbiota and Co‐Regulates Host AMPK Pathways

Abstract: These findings suggest a central mechanism via which SDF-diet may control TMA metabolism by gut microflora and co-regulate the AMPK pathways of the host.

Cited by 54 publications

References 50 publications

Potential correlation between carbohydrate‐active enzyme family 48 expressed by gut microbiota and the expression of intestinal epithelial AMP‐activated protein kinase β

Potential correlation between carbohydrate‐active enzyme family 48 expressed by gut microbiota and the expression of intestinal epithelial AMP‐activated protein kinase β

Background Diet Influences TMAO Concentrations Associated with Red Meat Intake without Influencing Apparent Hepatic TMAO-Related Activity in a Porcine Model

Inulin does not affect trimethylamine N‐oxide formation in mice with a high‐fat diet combined with choline and L‐carnitine

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