Hydrolysis of sugarcane bagasse with enzyme preparations fromAcrophialophora nainianagrown on different carbon sources

Abstract

the filamentous fungus Acrophialophora nainiana, isolated from a hot spring in Brazil, was grown in liquid culture on different cellulosic and lignocellulosic carbon sources for seven days and enzyme extracts were characterised with respect to their carbohydrase activity profile. the enzyme extracts obtained from growing A. nainiana on cellulose, dirty-cotton residue, sugarcane bagasse and banana stem were used in the hydrolysis of sugarcane bagasse untreated (ut), pre-treated by steam explosion (set) and pre-treated by acid-catalysed steam explosion (sat). the carbohydrase activity profile of the enzyme preparations varied significantly with the used carbon source. the highest enzyme activities, especially total cellulase (0.0132 iu) and xylanase (0.0774 iu) activities, were obtained with banana stem as the carbon source. Pectinase activity was produced on all carbon sources at comparable levels. on sugarcane bagasse, total cellulase activity on filter paper and pectinase activities were predominant, but a very low amount of xylanase and cMcase activity, 0.0011 iu and 0.0019 iu, respectively, was found. the exocellulase/endocellulase activity ratio (FPa sol /FPa insol ) of the cellulases produced varied between 1 and 4 depending on the substrate. the highest endocellulase activity (FPa insol ) content was obtained when grown on sugarcane bagasse. conversions to reducing sugars of the differently pre-treated sugarcane bagasse substrates with enzyme preparations from A. nainiana were in general low. the highest conversion to reducing sugars (~18%) was obtained for the set bagasse by the banana stem enzyme preparation, while conversions with the other enzyme preparations were below 5%. in most cases a very low conversion (below 1%) was obtained for the sat bagasse, but better conversions were achieved for the ut. these results are mainly attributable to the hydrolysis of the hemicellulose fraction and the low cellulase and b-glucosidase activities in the enzyme preparations. Hydrolysis data were also analysed and successfully fitted with a fractal kinetics model, and model parameters are discussed with respect to the carbon source used for A. nainiana enzyme production and substrate pre-treatment.Biocatal Biotransformation Downloaded from informahealthcare.com by The University of Manchester on 03/30/15For personal use only.

Brazilian Agro-industrial Wastes as Potential Textile and Other Raw Materials: a Sustainable Approach

“…6 ) (Bilatto et al 2020 ; de Oliveira Júnior et al 2020 ; Leão et al 2017 , 2020 ; Oliveira et al 2016 ; Pereira and Arantes 2018 , 2020 ). Figure 6 also highlights studies in which the plant works as a matrix for other textile fibers such as sisal for new biodegradable composites (de Castro et al 2021 ; Satyanarayana et al 2009 ); fibers are commonly used for reinforcement in composites (Cardoso et al 2017 ; De Lemos et al 2017 ; Dos Santos et al 2018 ; Ferreira et al 2019 ; Mulinari et al 2012 ); production of multifunctional biocatalysts (Bilal et al 2020 ; Silveira et al 2014a , b ); enzymes such as peroxidase (Queiroz et al 2018 ), amylase (Orlandelli et al 2017 ), cellulase (Do Nascimento and Coelho 2011 ), hemicellulase (Camassola & Dillon 2007 ), and xylanase (L. A. Oliveira et al 2006 ); and dye adsorption (Cunha et al 2018 ; B. C. S. Ferreira et al 2015 ; Giusto et al 2017 ; Meili et al 2019 ; Piffer et al 2020 ). In addition, Costa et al ( 2015 ) highlight the use of sugarcane as a material suitable for textile application and the development of bioproducts.…”

“…Other fruits, not shown in the diagram, stand out, such as apple, khaki, avocado, and pomegranate for the removal of dyes (Bazzo et al 2016 ; Bonetto et al 2021 ; Silveira et al 2014a , b ); the seeds of açai in the adsorption and obtaining of fibers and application in composites (de Oliveira et al 2019a , b ; Wataya et al 2016 , 2015 ; Zavarize 2021 ); cashew and acerola for bionanocomposites (Duarte et al 2015 ; Vieira Amorim et al 2021 ); cocoa husks for cellulose nanofiber (Souza et al 2019 ); and papaya and mango for the generation of enzymes for the textile industry (Okino-Delgado et al 2018 ). The cereal group also presents incidence of moreover materials, such as oat husks for the generation of nanofibrillated cellulose (Debiagi et al 2021 ).…”

Effect of Rhizospheric Fungus on Biological Control of Root Rot (Fusarium equiseti) Disease of Saposhnikovia divaricata

“…Antagonistic fungi mainly inhibit plant diseases through competition, lysis, and hyperparasitism [46]. Through the production of β-glucosidase, cellulose, xylanase [47,48], pectolases [49], and β-mannanase [50], the genus Acrophialophora was discovered to effectively inhibit the growth of plant pathogenic fungi. In this study, MR-57 culture filtrate inhibited F. equiseti mycelium and spore germination, resulting in mycelium malformation and rupture and a decreased spore germination rate.…”

Effect of Enhanced Organic Material Addition during Reductive Soil Disinfestation on Disease Resistance, Yield Increase, and Microbial Community in Flue-Cured Tobacco

“…Compared with the YCK and KCK treatments, an increased application of organic material (YG1.2 and KG1.2) led to a pronounced increase in the relative abundance of Actinobacteria, Chloroflexi, Firmicutes, and Acidobacillus at the phylum level, mirroring Huang et al [35] and Rao et al [33]'s observations. These bacterial phyla are pivotal for augmenting soil nutrient levels via plant residue degradation, lignin and protein decomposition, and humus formation [38][39][40]. Hence, the addition of organic material in the RSD process notably promoted nutrient levels in the rhizosphere soil of the continuous cropping of flue-cured tobacco.…”