Hydrolysis of chlorogenic acid in apple juice using a <i>p</i>‐coumaryl esterase of <i>Rhizoctonia solani</i>

Siebert, Mareike; Berger, Ralf G.; Pfeiffer, Franziska

doi:10.1002/jsfa.9940

Cited by 6 publications

(3 citation statements)

References 21 publications

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“…This is illustrated in Figure 2 b, which shows the chromatograms obtained with the benchtop nanoLC system for a standard solution containing a mixture of the tested compounds freshly prepared and 6 days after. The peak at 7.0 min was identified as caffeic acid, which is in agreement with the results reported by other authors [ 25 , 26 ]. As this compound could be resolved from those of the CGAs under the three tested chromatographic systems (see Figure 1 ), it was included in the study as a marker to detect the possible degradation of the CGAs.…”

Section: Resultssupporting

confidence: 92%

Hand-Portable Miniaturized Liquid Chromatography for the Determination of Chlorogenic Acids in Dietary Supplements

et al. 2022

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With the explosive growth of the dietary supplements industry, new demands have emerged that cannot be faced with the sophisticated instrumentation available in well-equipped laboratories. In particular, there is a demand for simplified and easy-to-use instruments, capable of providing results in short times of analysis. In this study, a hand-portable miniaturized liquid chromatograph (portable LC) has been tested for the determination of chlorogenic acids (CGAs) in products intended to supplement the diet and elaborated with green coffee extracts. CGAs offer several health benefits due to their antioxidant properties, and an increasing number of dietary supplements are marketed with claimed high contents of these compounds. The results obtained with the proposed portable LC approach have been compared with those obtained with two other miniaturized benchtop liquid chromatography instruments, namely, a capillary liquid chromatograph (capLC) and a nano liquid chromatograph (nanoLC). Although compared with the methods that used the benchtop instruments, the sensitivity attainable was lower, the portable LC instrument provided a comparable analytical performance for the quantification of the main GCAs at low mg g−1 levels, and it was clearly superior in terms of speed. The proposed portable LC-based method can be applied to assess the content and distribution profile of the predominant CGAs in this kind of dietary supplement. It can be also used to estimate the antioxidant power due to CGAs, as well as their preservation state.

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Section: Resultssupporting

confidence: 92%

Hand-Portable Miniaturized Liquid Chromatography for the Determination of Chlorogenic Acids in Dietary Supplements

et al. 2022

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“…CGA is an ester formed by the condensation of a molecule of caffeic acid and a molecule of quinic acid [7]. It is widely found in natural plants, such as sunflower seeds, eucommia, coffee beans and potatoes [8][9][10]. The CGA content in sunflower seeds can reach 71.4% of the total phenolic content in sunflower seeds and play a critically negative role in protein quality.…”

Section: Introductionmentioning

confidence: 99%

Heterologous Expression and Characterization of a Ferulic Acid Esterase from Aspergillus aculeatus with Potential Use in Sunflower Seed Processing

et al. 2021

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Hydrolytic activity for chlorogenic acid (CGA) has been recognized as an important side activity of some types of ferulic acid esterases. The purpose of this work was to enhance the efficient expression of ferulic acid esterase (FAE) and to explore its application in the processing of sunflower seed. Two novel FAEs from Aspergillus aculeatus (AaSD14) were expressed in genetically engineered E. coli BL21 (DE3), and their properties, including temperature, pH, metal ions and substrate specificity, were characterized after purification. Competitive CGA hydrolysis activity was observed in these recombined ferulic acid esterases (reFAEs) with reFAE1 of 246.37 U/g and reFAE2 of 340.95 U/g, which were 56.6 and 78.4 times higher than that of the wild strain (4.35 U/g), respectively. Meanwhile, the fermentation cycle was greatly shortened to 2.0 d. These reFAEs were recognized as type C FAE through substrate specificity assays. Treatment of sunflower seed protein (SSP) using reFAE2 resulted in a remarkable color change, from green to milk-white, confirming the activity of CGA biodegradation. Therefore, it shows certain potential in the processing of sunflower seed and other related foodstuffs.

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“…This should contribute to an improved digestibility without compromising the antioxidant capacity (Scheme 2a) [44]. The same reaction principle was successful in apple juice, without affecting the likewise contained fruit esters [45].…”

Section: Esterolytic Processesmentioning

confidence: 82%

Improved Foods Using Enzymes from Basidiomycetes

Berger

Ersoy

2022

Processes

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Within the kingdom of fungi, the division Basidiomycota represents more than 30,000 species, some with huge genomes indicating great metabolic potential. The fruiting bodies of many basidiomycetes are appreciated as food (“mushrooms”). Solid-state and submerged cultivation processes have been established for many species. Specifically, xylophilic fungi secrete numerous enzymes but also form smaller metabolites along unique pathways; both groups of compounds may be of interest to the food processing industry. To stimulate further research and not aim at comprehensiveness in the broad field, this review describes some recent progress in fermentation processes and the knowledge of fungal genetics. Processes with potential for food applications based on lipases, esterases, glycosidases, peptidases and oxidoreductases are presented. The formation and degradation of colourants, the degradation of harmful food components, the formation of food ingredients and particularly of volatile and non-volatile flavours serve as examples. In summary, edible basidiomycetes are foods—and catalysts—for food applications and rich donors of genes to construct heterologous cell factories for fermentation processes. Options arise to support the worldwide trend toward greener, more eco-friendly and sustainable processes.

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Hydrolysis of chlorogenic acid in apple juice using a p‐coumaryl esterase of Rhizoctonia solani

Cited by 6 publications

References 21 publications

Hand-Portable Miniaturized Liquid Chromatography for the Determination of Chlorogenic Acids in Dietary Supplements

Hand-Portable Miniaturized Liquid Chromatography for the Determination of Chlorogenic Acids in Dietary Supplements

Heterologous Expression and Characterization of a Ferulic Acid Esterase from Aspergillus aculeatus with Potential Use in Sunflower Seed Processing

Improved Foods Using Enzymes from Basidiomycetes

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