Hydrogenophaga aquatica sp. nov., isolated from a hot spring

Abstract

A polyphasic approach was used to characterize an aerobic, Gram-negative, rod-shaped bacterium (designated strain CC-KL-3) isolated from a hot spring. Phylogenetic analyses based on 16S rRNA genes indicated that strain CC-KL-3 showed highest sequence similarity to Hydrogenophaga bisanensis (97.7 %) and Hydrogenophaga atypica (97.6 %) and lower sequence similarity to other species (less than 97.6 %). The levels of DNA-DNA relatedness between strain CC-KL-3, H. bisanensis and H. atypica were estimated to be 13.0 and 8.7 % (the reciprocal value was 14.7 and 6.3 %). Strain CC-KL-3 was non-motile, without apparent flagella and able to grow between 15-42 °C (optimal 30 °С), pH 6.0-8.0 (optimal 7.0) and 0-2 % (w/v) NaCl (optimal 0 %). The DNA G+C content was 61.4 mol% and the major quinone system was ubiquinone (Q-8). The polyamine profile revealed the predominance of 2-hydroxyputrescine and putrescine and the dominant cellular fatty acids were C16 : 0 (28.9 %), C16 : 1ω7c/C16 : 1ω6c (41.4 %) and C18 : 1ω7c/C18 : 1ω6c (11.9 %). These data corroborated the affiliation of strain CC-KL-3 to the genus Hydrogenophaga. Based on the distinct phylogenetic, phenotypic and chemotaxonomic traits, and the results of comparative 16S rRNA gene sequence analysis, strain CC-KL-3 is considered to represent a novel species of the genus Hydrogenophaga, affiliated to the family Comamonadaceae, for which the name Hydrogenophaga aquatica sp. nov. is proposed. The type strain is CC-KL-3 (=BCRC 80937=JCM 31216).

Hydrogenophaga aromaticivorans sp. nov., isolated from a para-xylene-degrading enrichment culture, capable of degrading benzene, meta- and para-xylene

“…Species of Hydrogenophaga are positive for oxidase activity and show variable activity for catalase [1]. The main cellular fatty acids are C 16 : 0 and C 16 : 1 ω7 c and/or C 16 : 1 ω6 c [3–5]. The DNA G+C content ranges from 61.3 to 69.9 mol% [5, 6] and the predominant quinone system is ubiquinone-8 (Q-8) [1].…”

“…The DNA G+C content ranges from 61.3 to 69.9 mol% [5,6] and the predominant quinone system is ubiquinone-8 (Q-8) [1]. At the time of writing, 15 species of the genus Hydrogenophaga have been validly described, which were isolated from diverse habitats, such as wastewater [7][8][9], activated sludge [2][3][4][5][6][7][8][9], soil [1,10], freshwater ecosystems [3,4,11] or even pacific oyster [5]. Additionally, members of the genus Hydrogenophaga can be key benzene-degrading bacteria in petroleum hydrocarbon contaminated subsurface environments [12].…”

The Chemical Characteristic and Microbial Diversity of the Hot Spring at Phusang National Park

“…T4, Hydrogenophaga flava, Hydrogenophaga taeniospiralis, etc. and unassigned Hydrogenophaga that need to be evaluated in a further study (Lin et al, 2017).…”

Microbial diversity of drilling fluids from 3000 m deep Koyna pilot borehole provides insights into the deep biosphere of continental earth crust

“…Drilling fluids' microbial diversity as a marker for deep life was studied during a number of drilling operations. Drilling fluid obtained from deep drilling at the Cretaceousage Piceance basin in Colorado, Triassic-age Taylorsville basin in Virginia, Chinese continental scientific drilling operations (CCSD) at a 240 Ma old ultra-high pressure metamorphic (UHPM) belt located in Jiangsu Province in China and during the first riser drilling operation on the deep-earth research vessel Chikyu was used for microbiological studies (Liu et al, 1997;Roh et al, 2002;Zhang et al, 2005Zhang et al, , 2006Masui et al, 2008). The presence of microorganisms able to survive the extreme oligotrophic conditions of the deep subsurface was detected in these studies.…”

“…The presence of microorganisms able to survive the extreme oligotrophic conditions of the deep subsurface was detected in these studies. Several strains of an extremely thermophilic, anaerobic chemoorganotroph bacterium Thermoanaerobacter ethanolicus have been previously isolated from the fluids obtained during drilling in the Piceance basin in Colorado and Taylorsville basin in Virginia at different depths of the geological formation (Liu et al, 1997;Roh et al, 2002). This particular organism was also retrieved from the DF samples of CCSD.…”