Hydrogen Sulphide Production in Healthy and Ulcerated Gastric Mucosa of Rats

Bronowicka-Adamska, Patrycja; Wróbel, Maria; Magierowski, Marcin; Magierowska, Katarzyna; Kwiecień, Sławomir; Brzozowski, Tomasz

doi:10.3390/molecules22040530

Cited by 14 publications

(14 citation statements)

References 25 publications

(30 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Attention must also be paid to the fact that the beneficial effect of hydrogen sulfide formed in the intestine on the general condition of the body, e.g., regulation of arterial pressure, has not been taken into account [55,56]. Similarly, the beneficial H 2 S role in inflammation, which can act as an antioxidant [57].…”

Section: Discussionmentioning

confidence: 99%

Hydrogen Sulfide Effects on the Survival of Lactobacilli with Emphasis on the Development of Inflammatory Bowel Diseases

et al. 2019

View full text Add to dashboard Cite

The gut microbiota is a complex component of humans that depends on diet, host genome, and lifestyle. The background: The study purpose is to find relations between nutrition, intestinal lactic acid bacteria (LAB) from various environments (human, animal intestine, and yogurt) and sulfate-reducing microbial communities in the large intestine; to compare kinetic growth parameters of LAB; and to determine their sensitivity to different concentration of hydrogen sulfide produced by intestinal sulfate-reducing bacteria. Methods: Microbiological (isolation and identification), biochemical (electrophoresis), molecular biology methods (DNA isolation and PCR analysis), and statistical processing (average and standard error calculations) of the results were used. The results: The toxicity of hydrogen sulfide produced by sulfate-reducing bacteria, the survival of lactic acid bacteria, and minimal inhibitory concentrations (MIC) were determined. The measured hydrogen sulfide sensitivity values were the same for L. paracasei and L. reuteri (MIC > 1.1 mM). In addition, L. plantarum and L. fermentum showed also a similar sensitivity (MIC > 0.45 mM) but significantly (p < 0.05) lower than L. reuteri and L. paracasei (1.1 > 0.45 mM). L. paracasei and L. reuteri are more sensitive to hydrogen sulfide than L. fermentum and L. plantarum. L. pentosus was sensitive to the extremely low concentration of H2S (MIC > 0.15 mM). Conclusions: The Lactobacillus species were significantly sensitive to hydrogen sulfide, which is a final metabolite of intestinal sulfate-reducing bacteria. The results are definitely helpful for a better understanding of complicated interaction among intestinal microbiota and nutrition.

show abstract

Section: Discussionmentioning

confidence: 99%

Hydrogen Sulfide Effects on the Survival of Lactobacilli with Emphasis on the Development of Inflammatory Bowel Diseases

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The MPST activity was assayed according to the method of Valentine and Frankelfeld 1974 [13] with some modification as described by Wróbel et al [14] and Bronowicka-Adamska et al [15]. The enzyme activity was expressed as nmoles of pyruvate produced during 1 min incubation at 37 °C/1 mg of protein.…”

Section: Mpst Activitymentioning

confidence: 99%

“…Sulfane sulfur was determined by the method of Wood [20] following the procedure described by Bronowicka-Adamska et al [15]. The method is based on cold cyanolysis and colorimetric detection of ferric thiocyanate complex ion.…”

Section: Sulfane Sulfur Levelmentioning

confidence: 99%

Expression and activity of hydrogen sulfide generating enzymes in murine macrophages stimulated with lipopolysaccharide and interferon-γ

et al. 2019

Self Cite

View full text Add to dashboard Cite

Murine macrophages of the J774A.1 line are hydrogen sulphide-producing cells with the primary role of γ-cystathionase (CTH) and secondary role of 3-mercaptopyruvate sulfurtransferase (limited by cysteine availability) and with a negligible role of cystathionine β-synthase (CBS) in H 2 S generation. J774A.1 cells stimulation with lipopolysaccharide (LPS) or interferongamma (IFNγ) resulted in decreased H 2 S levels after 24 h of incubation; however, they were restored to the control level after 48 h. Negligible CBS expression and activity in J774A.1 cells can result in homocysteine availability for CTH-catalyzed, H 2 S-generating reactions. This was supported by an increased CTH expression (IFNγ, 24 h and 48 h, and LPS, 48 h) and activity (24 h, LPS) in the stimulated cells. The results confirm the suggested feedback regulation between CBS and CTH.

show abstract

“…TST activity was assayed in the tissue homogenates according to Sörbo (Sörbo, 1955), with modifications described by Bronowicka-Adamska and others (Bronowicka-Adamska et al, 2017). The enzyme units were defined as nmoles of SCNgenerated per minute per 1 mg of protein at 20°C under the prescribed assay conditions.…”

Section: Scheme 1 H 2 S Metabolism In the Mammalian Pancreasmentioning

confidence: 99%

Hydrogen sulfide formation in experimental model of acute pancreatitis

et al. 2019

Self Cite

View full text Add to dashboard Cite

Acute pancreatitis (AP) is a disease defined as acute or chronic inflammatory process of the pancreas characterized by premature activation of digestive enzymes within the pancreatic acinar cells and causing pancreatic auto-digestion. In mammalian tissues, H2S is synthesized endogenously from L-cysteine in regulated enzymatic pathways catalyzed by pyridoxal phosphate-dependent enzymes: cystathionine beta - synthase (CBS), gamma - cystathionase (CTH) and cysteine aminotransferase (CAT) coupled with 3-mercaptopyruvate sulfurtransferase (MPST). In the mitochondria, hydrogen sulfide is oxidized to sulfite, which is then converted to thiosulfate (sulfane sulfur-containing compound) by thiosulfate sulfurtransferase (rhodanese; TST). The activity and the expression of CBS, CTH, MPST, and TST have been determined in vivo in pancreas of control rats, rats with acute pancreatitis and sham group. Levels of low-molecular sulfur compounds such as reduced and oxidized glutathione, cysteine, cystine and cystathionine were also determined. The study showed the significant role of MPST in H2S metabolism in pancreas. Stress caused by the surgery (sham group) and AP cause a decrease in H2S production associated with a decrease of MPST activity and expression. Markedly higher level of cysteine in the AP pancreas may be caused by a reduced rate of cysteine consumption in reaction catalyzed by MPST but it can also be a sign of the processes of proteolysis occurring in the changed tissue.

show abstract

Hydrogen Sulphide Production in Healthy and Ulcerated Gastric Mucosa of Rats

Cited by 14 publications

References 25 publications

Hydrogen Sulfide Effects on the Survival of Lactobacilli with Emphasis on the Development of Inflammatory Bowel Diseases

Hydrogen Sulfide Effects on the Survival of Lactobacilli with Emphasis on the Development of Inflammatory Bowel Diseases

Expression and activity of hydrogen sulfide generating enzymes in murine macrophages stimulated with lipopolysaccharide and interferon-γ

Hydrogen sulfide formation in experimental model of acute pancreatitis

Contact Info

Product

Resources

About