Hydrogen Sulfide Promotes Adipogenesis in 3T3L1 Cells

Abstract

The effect of hydrogen sulfide (H2S) on differentiation of 3T3L1-derived adipocytes was examined. Endogenous H2S was increased after 3T3L1 differentiation. The expression of the H2S-synthesising enzymes, cystathionine γ-lyase (CSE), cystathionine β-synthase (CBS) and 3-mercaptopyruvate sulfurtransferase (3-MST), was increased in a time-dependent manner during 3T3L1 differentiation. Expression of genes associated with adipogenesis related genes including fatty acid binding protein 4 (FABP4/aP2), a key regulator of this process, was increased by GYY4137 (a slow-releasing H2S donor compound) and sodium hydrosulfide (NaHS, a classical H2S donor) but not by ZYJ1122 or time-expired NaHS. Furthermore expression of these genes were reduced by aminooxyacetic acid (AOAA, CBS inhibitor), DL-propargylglycine (PAG, CSE inhibitor) as well as by CSE small interference RNA (siCSE) and siCBS. The size and number of lipid droplets in mature adipocytes was significantly increased by both GYY4137 and NaHS, which also impaired the ability of CL316,243 (β3-agonist) to promote lipolysis in these cells. In contrast, AOAA and PAG had the opposite effect. Taken together, we show that the H2S-synthesising enzymes CBS, CSE and 3-MST are endogenously expressed during adipogenesis and that both endogenous and exogenous H2S modulate adipogenesis and adipocyte maturation.

Arylfluorosulfates Inactivate Intracellular Lipid Binding Protein(s) through Chemoselective SuFEx Reaction with a Binding Site Tyr Residue

“…Since HeLa cells do not express FABP4, we tested this hypothesis using 3T3-L1 mouse adipocytes, which express FABP4, but not FABP3, at high levels. 79 Living cells were treated with probe 1 (40 µM for 16 h at 37 °C) and cell lysates were reacted with rhodamine-azide using a CuAAC click reaction. SDS-PAGE in-gel fluorescence analysis revealed that probe 1 covalently labeled a 15 kDa band (Figure S13a).…”

Hydrogen sulfide modulates high glucose‑induced NLRP3 inflammasome activation in 3T3‑L1 adipocytes

“…In the cardiovascular system, H 2 S is suggested to inhibit the development of atherosclerosis through suppression of inflammation (25,26). Cystathionine-γ-lyase (CSE) and cystathionine-β-synthase (CBS) are enzymes responsible for the synthesis of H 2 S. It has been reported that both CSE and CBS are expressed in adipose tissues and adipocytes (27,28). Pan et al (29) demonstrated that HG inhibits expression of CSE, and thus the production of H 2 S, in adipocytes.…”

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“…An adipocytes maturation is accompanied by the H 2 S biosynthesis genes overexpression. Moreover, this study indicates the main role of H 2 S in lipoge nesis [10]. It describes a CBS-dependent translocation of Sp1 to the nucleus and the SREBP gene promoter binding in the experiments with the ovarian cancer cells [11].…”

“…It is known that Sterol regulatory element-binging protein (SREBP) is a transcription factor which induces the transcription of cholesterol biosynthesis genes [69]. There are several stu dies which show that H 2 S stimulates the cholesterol biosynthesis through SREBP regulation [9][10][11]. For example, a treatment of pancreas beta-cells with H 2 S donors, or the transfection with constitutional active cse gene induced up-regulation of (SREBP)-1c [9].…”

“…The second reaction is catalyzed by Cystationine-beta-Synthase (CBS) others are catalyzed by Cystathionine-gamma-Lyase (CSE) [5,6].It is now known that H 2 S is a substrate for post-translational modification of a protein, called sulfhydration [7]. Furthermore, H 2 S is an important element of the antioxidant system [8], as well as a regulator of the lipid [9][10][11][12] and NO metabolism [13][14][15][16].…”