Hydrogen sulfide oxidation under anoxic conditions by a nitrate-reducing, sulfide-oxidizing bacterium isolated from the Mae Um Long Luang hot spring, Thailand

Watsuntorn, Wannapawn; Ruangchainikom, Chalermchai; Rene, Eldon R.; Lens, Piet N.L.; Chulalaksananukul, Warawut

doi:10.1016/j.ibiod.2017.06.013

Cited by 26 publications

(12 citation statements)

References 37 publications

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“…This is understandable that the SOB inhabits the hot spring has undergone a physiological adaptation during the storage time as well as during the enrichment process at room temperature and thus isolate AH25 was classified as a mesophilic SOB. This was in line with Watsuntorn et al (2017) study, in which only recorded an optimal temperature at 35 °C in SOB of strain MAL 1HM19 which was isolated from hot spring with in situ temperature at 70 °C. In fact, the ability of the potential SOB isolates to grow up to 45 °C was due to the natural habitat of bacteria itself which is from the hot spring.…”

Section: Discussionsupporting

confidence: 91%

“…However, in this study, the formation of sulphuric acid has reached a level in which is not favourable for SOB and brings the growth to a halt state. Similar to the works of Watsuntorn et al (2017), which have observed no bacteria growth at a pH below 7.0 of SOB strain MAL 1HM19 isolated from hot spring in Thailand. Moreover, Hydrogenobater sp.…”

Section: Discussionsupporting

confidence: 88%

“…They can be found in the environment where the sulphur compounds are in higher concentrations which can provide good habitats for SOB (Druschel et al, 2003). Hot spring is believed to have a high amount of saturated sulphur and various reduced sulphur compounds in the water due to the mud or the sediment are composed of sulphur compounds, which can potentially act as electron donors for SOB growth (Watsuntorn et al, 2017). Additionally, microorganisms that live in hot spring water have minimal requirements for nutrients and their metabolic activities only depend on the biogeochemical cycle such as sulphur and other mineral contents (Skirnisdottir et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

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Isolation and Characterisation of Sulphur Oxidizing Bacteria Isolated from Hot Spring in Malaysia for Biological Deodorisation of Hydrogen Sulphide in Chicken Manure

Hidayat¹,

Saud²,

Samsudin³

2017

Med Pet

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In this study, the isolation of sulphur oxidising bacteria (SOB) from hot spring in Malaysia was carried out in an enrichment culture using sodium thiosulphate as a sole energy and CO 2 as a sole carbon source. A total number of 80 SOB isolates were obtained from the agar plate and considered as positive SOB due to their abilities using thiosulphate for growth. All the isolates were initially screened for their fast growths in liquid medium and 13 isolates were selected for another screening process. Three SOB isolates namely isolate AH18, AH25, and AH28 were selected based on their abilities to grow faster, produce the highest sulphate ion and reducing the pH in the growth medium. The cells were Gram-negative and short rod-shaped. The effects of various variables including temperature (25-45 °C), pH (4-9), sodium thiosulphate concentrations (4-100 mM) and metabolic characteristic were evaluated on bacterial growth and their sulphur oxidation activities. The optimum pH of all the potential isolates occurred at pH 8.0. Meanwhile, the optimum temperature for isolate AH18, AH25 and AH28 occurred at 45 °C, 30 °C, and 30-45 °C, respectively. The three isolates were classified as facultative chemolithotroph with the capability of growth in thiosulphate concentration as high as 100 mM. Therefore, given the ability in the oxidation of thiosulphate, temperature and pH adaptabilities, with the metabolic flexibilities of isolates AH18, AH25, and AH28 could be a good H 2 S biological deodorizing candidate.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

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Isolation and Characterisation of Sulphur Oxidizing Bacteria Isolated from Hot Spring in Malaysia for Biological Deodorisation of Hydrogen Sulphide in Chicken Manure

Hidayat¹,

Saud²,

Samsudin³

2017

Med Pet

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“…physicochemical treatment approaches (electrocoagulation, adsorption, thermal and catalytic oxidation, ozonation, membrane separation) have been quite promising in terms of faster conversion rate and huge turnover, except for its high cost of maintenance (Azizan et. al., 2016), energy consumption and consequential production of secondary pollutants (Watsuntorn, et. al., 2017;Zytoon, et.…”

Section: Characterization and Quality Analysis Of Petroleum Refinery mentioning

confidence: 99%

Integrated co-cultured bacterial strains capabilities to aqueous sulfide and chemical oxygen demand mitigation from high-strength petroleum refinery wastewater

Mani¹,

Aziz²

2019

Nig. J. Biotechnol

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The heterogeneous nature of petroleum refinery based wastewater (PRW) couple with the documented paucity of the classical physicochemical mitigation approaches were behind the quest for a cost-effective and ecofriendly alternative with minimum negative effects. The toxic and inhibitory substances contained in PRW have made its treatment strenuous using a simple pure culture. A novel integrated bacterial mixed culture (BMC), Pseudomonas putida (ATCC 49128) and Bacillus cereus (ATCC 14579) with traceable imprints in biodegradation of high-strength PRW was proposed as a suitable alternative for sulfide oxidation and COD reduction with a potential alternative to classical physicochemical mitigation approaches. Comparative biodegradation potential of two acclaimed bacteria mixed-culture regimen was evaluated. The degree of inhibition cast was assessed based on the effectiveness of BMC to remove the targeted compounds (aqueous hydrogen sulfide and COD) within the assigned period of 8 hours hydraulic retention time (HRT). The PRW was found to contained initial concentration of 8,155 ppm COD, and 500 ppm sulfide, coupled with other refractory substances. The experiment was repeated batch-wise under defined optimal conditions of 0.25 L/min O , 2 o the temperature of 30 C, agitation of 140 rpm at 8 hours retention time. The results obtained indicated 97.47±1.7 % (mg/L) COD reduction and 99.75±1.8 (ppm) sulfide removal efficiency (RE). In addition, the SEM analysis revealed further the potential of BMC to degrade sulfide from PRW to economically feasible elemental sulfur species, while EDXS demonstrated a proportionate elemental composition revealing appreciable sulfur content and high organic carbon accumulation. Despite the expected toxicity and inhibitory effect of the medium, an overwhelming biodegradation was achieved disproportionately, hence the model can be suggested for further research of repulsive wastewaters.

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“…T he genus Paracoccus, phylogenetically classified in the class Alphaproteobacteria, comprises Gram-negative, nonmotile bacteria which belong to the facultative chemolithotrophic sulfide-oxidizing bacteria (1). They have been isolated from many sources, including bioreactors, sludge, aerobic granules, sediments, and water from hot springs (2)(3)(4)(5)(6)(7)(8). Recently, Paracoccus versutus MAL 1HM19, isolated from the Mae Um Long Luang hot spring (Thailand), was reported for its unique ability to simultaneously convert H 2 S and NO 3 Ϫ to sulfate (SO 4 2-) and dinitrogen (N 2 ) via the H 2 S oxidation and NO 3 Ϫ reduction pathways (8), respectively.…”

mentioning

confidence: 99%

Draft Genome Sequence and Annotation of Paracoccus versutus MAL 1HM19, a Nitrate-Reducing, Sulfide-Oxidizing Bacterium

Watsuntorn

Kojonna

Rene

et al. 2020

Microbiol Resour Announc

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Hydrogen sulfide oxidation under anoxic conditions by a nitrate-reducing, sulfide-oxidizing bacterium isolated from the Mae Um Long Luang hot spring, Thailand

Cited by 26 publications

References 37 publications

Isolation and Characterisation of Sulphur Oxidizing Bacteria Isolated from Hot Spring in Malaysia for Biological Deodorisation of Hydrogen Sulphide in Chicken Manure

Isolation and Characterisation of Sulphur Oxidizing Bacteria Isolated from Hot Spring in Malaysia for Biological Deodorisation of Hydrogen Sulphide in Chicken Manure

Integrated co-cultured bacterial strains capabilities to aqueous sulfide and chemical oxygen demand mitigation from high-strength petroleum refinery wastewater

Draft Genome Sequence and Annotation of Paracoccus versutus MAL 1HM19, a Nitrate-Reducing, Sulfide-Oxidizing Bacterium

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