Hydrogen sulfide negatively regulates cd-induced cell death in cucumber (Cucumis sativus L) root tip cells

Luo, Shilei; Tang, Zhongqi; Yu, Jiangnan; Liao, Weibiao; Xie, Jianming; Lv, Jian; Zhi, Feng; Dawuda, Mohammed Mujitaba

doi:10.1186/s12870-020-02687-8

Cited by 32 publications

(16 citation statements)

References 78 publications

(78 reference statements)

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“…Increased H 2 O 2 and MDA production during short-term exposure of cucumber cell cultures to Cd 2+ has been reported previously [47]. Many studies have revealed that a large number of Cd-responsive genes may be involved in ROS signal transduction [47]. The relevant signal transduction pathways include Ca-calmodulin system, hormones, and mitogenactivated protein kinase (MAPK) phosphorylation cascade, which converge by activating Cd-related ROS genes [34].…”

Section: Discussionmentioning

confidence: 89%

“…Cadmium pollution has attracted worldwide attention [ 21 ]. Cd toxicity can cause ROS elevation in plants, oxidative damage, lipid peroxidation, and growth inhibition [ 47 ]. Increased H 2 O 2 and MDA production during short-term exposure of cucumber cell cultures to Cd 2+ has been reported previously [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

“…Cd toxicity can cause ROS elevation in plants, oxidative damage, lipid peroxidation, and growth inhibition [ 47 ]. Increased H 2 O 2 and MDA production during short-term exposure of cucumber cell cultures to Cd 2+ has been reported previously [ 47 ]. Many studies have revealed that a large number of Cd-responsive genes may be involved in ROS signal transduction [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

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RNA-Seq Identification of Cd Responsive Transporters Provides Insights into the Association of Oxidation Resistance and Cd Accumulation in Cucumis sativus L.

Feng¹,

Shen

Xu³

et al. 2021

Antioxidants

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Greenhouse vegetable production (GVP) has grown rapidly and has become a major force for cucumber production in China. In highly intensive GVP systems, excessive fertilization results in soil acidification, increasing Cd accumulation and oxidative stress damage in vegetables as well as increasing health risk of vegetable consumers. Therefore, enhancing antioxidant capacity and activating the expression level of Cd transporter genes seem to be feasible solutions to promote plant resistance to Cd stress and to reduce accumulated Cd concentration. Here, we used transcriptomics to identify five cucumber transporter genes (CsNRAMP1, CsNRAMP4, CsHMA1, CsZIP1, and CsZIP8) in response to cadmium stress, which were involved in Cd transport activity in yeast. Ionomics, gene expression, and REDOX reaction level association analyses have shown that the transcript of CsNRAMP4 was positively correlated with Cd accumulation and antioxidant capacity of cucumber roots. The expression level of CsHMA1 was negatively correlated with Cd-induced antioxidant capacity. The overexpression of CsHMA1 significantly relieved Cd stress-induced antioxidant activities. In addition, shoots with high CsHMA2 expression remarkably presented Cd bioaccumulation. Grafting experiments confirmed that CsHMA1 contributed to the high antioxidant capacity of cucumber, while CsHMA2 was responsible for the transport of Cd from the roots to the shoots. Our study elucidated a novel regulatory mechanism for Cd transport and oxidative damage removal in horticultural melons and provided a perspective to regulate Cd transport artificially by modulating Cd accumulation and resistance in plants.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

RNA-Seq Identification of Cd Responsive Transporters Provides Insights into the Association of Oxidation Resistance and Cd Accumulation in Cucumis sativus L.

Feng¹,

Shen

Xu³

et al. 2021

Antioxidants

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“…The function of H 2 S in plants subject to Cd-related stress has been extensively studied. Hydrogen sulfide could alleviate the Cd-induced inhibition of root growth in Arabidopsis [69], Medicago sativa [67,77], Brassica rapa [68,70,76], Setaria italica [71], Cucumis sativus [72,75], Solanum lycopersicum [74], and Hordeum vulgare [73]. Furthermore, the suppression of plant root growth caused by other heavy metals, such as chromium (Cr), lead (Pb), mercury (Hg), and nickel (Ni), could also be relieved when exposed to H 2 S [73,78,79,[82][83][84]92,93].…”

Section: Heavy Metalsmentioning

confidence: 99%

The Role of Hydrogen Sulfide in Plant Roots during Development and in Response to Abiotic Stress

Chen

et al. 2022

IJMS

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Hydrogen sulfide (H2S) is regarded as a “New Warrior” for managing plant stress. It also plays an important role in plant growth and development. The regulation of root system architecture (RSA) by H2S has been widely recognized. Plants are dependent on the RSA to meet their water and nutritional requirements. They are also partially dependent on the RSA for adapting to environment change. Therefore, a good understanding of how H2S affects the RSA could lead to improvements in both crop function and resistance to environmental change. In this review, we summarized the regulating effects of H2S on the RSA in terms of primary root growth, lateral and adventitious root formation, root hair development, and the formation of nodules. We also discussed the genes involved in the regulation of the RSA by H2S, and the relationships with other signal pathways. In addition, we discussed how H2S regulates root growth in response to abiotic stress. This review could provide a comprehensive understanding of the role of H2S in roots during development and under abiotic stress.

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“…The homogenate was centrifuged at 16,000 × g for 20 min at 4 • C, and the supernatant was used for the assays. The activities of SOD, POD, and APX were determined according to established protocols (Luo et al, 2020). Nicotinamide adenine dinucleotide phosphate (NADPH) content was determined by following the method described previously, with slight modifications (Matsumura and Miyachi, 1980;Wei et al, 2019).…”

Section: Analysis Of Ros Homeostasismentioning

confidence: 99%

Gm6PGDH1, a Cytosolic 6-Phosphogluconate Dehydrogenase, Enhanced Tolerance to Phosphate Starvation by Improving Root System Development and Modifying the Antioxidant System in Soybean

Zheng

et al. 2021

Front. Plant Sci.

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Phosphorus plays an important role in plant growth and development, and is an important limiting factor for crop yield. Although previous studies have shown that 6-phosphogluconate dehydrogenase (6PGDH) plays an important role in plant resistance to adversity, its response to low phosphorus (P) stress remains unknown. In this study, we reported the cloning and characterization of a cytosolic 6PGDH gene, Gm6PGDH1, which enhanced the tolerance to phosphate (Pi) starvation by improving root system development and modifying the antioxidant system in transgenic plants. Gm6PGDH1 was highly expressed in the root at full bloom stage, and strongly induced by Pi starvation. The results from intact soybean composite plant and soybean plant, both containing a Gm6PGDH1-overexpressing construct, showed that Gm6PGDH1 was involved in root system development, and subsequently affected P uptake under Pi-deficient conditions. Meanwhile, the accumulation of reactive oxygen species (ROS) in the root tip of transgenic soybean was reduced, and the activity of ROS-scavenging enzymes was enhanced compared with those of the wild type under Pi-deficient conditions. Interestingly, we found that the overexpression of Gm6PGDH1 weakened the response of several other important Pi-answer genes to Pi starvation, such as some purple acid phosphatases (PAPs) and redox-related genes. In addition, the results from a virus-induced gene silencing (VIGS) indicated that Gm6PGDH1 might have functional redundancy in soybean, and the results from a heterogeneous transformation system showed that overexpressing Gm6PGDH1 also enhanced tolerance to Pi starvation in transgenic Arabidopsis. Together, these results suggested the great potential of Gm6PGDH1 in crop breeding for low Pi tolerance.

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Hydrogen sulfide negatively regulates cd-induced cell death in cucumber (Cucumis sativus L) root tip cells

Cited by 32 publications

References 78 publications

RNA-Seq Identification of Cd Responsive Transporters Provides Insights into the Association of Oxidation Resistance and Cd Accumulation in Cucumis sativus L.

RNA-Seq Identification of Cd Responsive Transporters Provides Insights into the Association of Oxidation Resistance and Cd Accumulation in Cucumis sativus L.

The Role of Hydrogen Sulfide in Plant Roots during Development and in Response to Abiotic Stress

Gm6PGDH1, a Cytosolic 6-Phosphogluconate Dehydrogenase, Enhanced Tolerance to Phosphate Starvation by Improving Root System Development and Modifying the Antioxidant System in Soybean

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