Hydrogen‐rich water attenuates experimental periodontitis in a rat model

Abstract

Aim Reactive oxygen species (ROS) contribute to the development of periodontitis. As molecular hydrogen can act as a scavenger of ROS, we examined the effects of treatment with hydrogen‐rich water on a rat model of periodontitis. Material & Methods A ligature was placed around the maxillary molars for 4 weeks to induce periodontitis, and the animals were given drinking water with or without hydrogen‐rich water. Results The rats with periodontitis which were treated with pure water showed a time‐dependent increase in serum ROS level. Compared with the rats without periodontitis, the periodontitis‐induced rats which were given pure water also showed polymorphonuclear leucocyte infiltration and alveolar bone loss at 4 weeks. Hydrogen‐rich water intake inhibited an increase in serum ROS level and lowered expression of 8‐hydroxydeoxyguanosine and nitrotyrosine in the periodontal tissue at 4 weeks. Such conditions prevented polymorphonuclear leucocyte infiltration and osteoclast differentiation following periodontitis progression. Furthermore, inflammatory signalling pathways, such as mitogen‐activated protein kinases, were less activated in periodontal lesions from hydrogen‐rich water‐treated rats as compared with pure water‐treated rats. Conclusion Consuming hydrogen‐rich water might be beneficial in suppressing periodontitis progression by decreasing gingival oxidative stress.

Effects of hydrogen-rich water on aging periodontal tissues in rats

“…In our previous study12, drinking hydrogen-rich water suppressed osteoclast differentiation following periodontal inflammation. In this study, drinking hydrogen-rich water lowered the number of TRAP-positive osteoclasts on the alveolar bone surface with aging.…”

“…Clinical and animal studies have demonstrated that drinking hydrogen-rich water provides numerous health benefits, such as in regards to the serum lipid profiles of those at risk for metabolic syndrome78, quality of life after radiation exposure9, oxidative damage in patients with chronic hepatitis B10 and nonalcoholic steatohepatitis related hepatocarcinogenesis11. In a previous study, we also found that drinking hydrogen-rich water suppressed ligature-induced periodontal inflammation in a rat model12. Notably, these studies mainly focused on the effects of hydrogen-rich water on human disease status and animal disease models.…”

Visualization of Oxidative Stress Induced by Experimental Periodontitis in Keap1-Dependent Oxidative Stress Detector-Luciferase Mice

“…In this study, the OKD48 transgenic mice actually permitted the visualization of oxidative stress due to periodontitis. We previously reported that oxidative stress due to periodontitis affects systemic conditions, and that antioxidants attenuated oxidative stress and inflammation [17,18,19]. Using this model, in the future, we may be able to visualize systemic oxidative stress by periodontitis in living animals, which may allow us to address various issues regarding oxidative stress in human chronic periodontitis and antioxidant development.…”

Ethical guidelines, animal profile, various animal models used in periodontal research with alternatives and future perspectives

“…Larger animals were reserved as the last choice for validation of newer surgical techniques prior to the use in human beings. [171819202122]…”