Hydrogen-rich saline attenuates ischemia–reperfusion injury in skeletal muscle

Huang, Tianlong; Wang, Wangchun; Tu, Chao; Yang, Zhenyu; Bramwell, Donald; Sun, Xuejun

doi:10.1016/j.jss.2014.12.016

Cited by 36 publications

(29 citation statements)

References 51 publications

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“…The MDA occurs naturally and is commonly used as a biomarker to measure the level of oxidative stress in an organism [18,19], especially in I/R injury in both myocardial [20] and skeletal muscles [21]. In this regard, the MDA was tested and the results showed ( Table 1) that, compared with sham, the amount of MDA was dramatically increased (4.94 ± 0.53 nM, P < 0.01) in the plasma of ischemic rats, which was further enhanced, at least double in amount, time-dependently following reperfusion in either I/R and saline-treated models and peaked at 8 h after I/R.…”

Section: Effects Of Msoda On Plasma Mda Before and After Ischemia/repmentioning

confidence: 99%

“…Since 2003, the MPO has long been served as a sensitive predictor for I/R injury of both cardiac and skeletal muscles [21,22]. To evaluate the injured level of skeletal muscle after I/R, the MPO was measured and the averaged data showed that increased MPO was observed in ischemic tissue (0.68 ± 0.05, P < 0.05 vs sham) and greatly increased from 150% up to 350% at 2 h and 8 h followed by the reperfusion with a similar trends during entire reperfusion compared with the alternation of MDA in the same preparation (Table 1).…”

Section: Effects Of Msoda Tissue Mpo Before and After Ischemia/reperfmentioning

confidence: 99%

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Protective Effects of Modeled Superoxide Dismutase Coordination Compound (MSODa) Against Ischemia/Reperfusion Injury in Rat Skeletal Muscle

Wang¹,

Tian²,

Xu³

et al. 2015

Cell Physiol Biochem

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Background/Aim: Ischemia/reperfusion (I/R) injury of skeletal muscles is common pathophysiology during surgeries and the superoxide dismutase (SOD) plays a critical role in this process. SOD-modeled coordination compound (MSODa) may simulate the protective effects as SOD. Methods: Therefore, this study was designed to explore the protective effects and underlying mechanism of MSODa on malondialdehyde (MDA) and integrin-β2 (CD11b/CD18) in plasma, myeloperoxidase (MPO) and intercellular cell adhesion molecule-1 (ICAM-1) in tissue, and morphological changes before and after I/R injury. The rat model of I/R in hind limb was established and randomly divided into sham, ischemia, I/R, I/R-treated with saline, SOD, and MSODa, respectively. Results: These results showed that averaged values for MDA, MPO, CD11b/CD18, and ICAM-1 were significantly increased (P < 0.01 vs ischemia alone) in a time-dependent fashion along with marked tissue remodeling, such as abnormal arrangement of muscular fibers, interstitial edema, vasodilation with no-reflow, inflammatory cells adherent and infiltration, structural changes in mitochondrial, and decrease in glycogens as well. However, all parameter changes induced by I/R injury were reversed, at least partially, by MSODa and SOD treatments and intriguingly, the beneficial/protective effects of MSODa was superior to SOD with an early onset. Conclusion: This novel finding demonstrates that MSODa improves I/R injury of skeletal muscles due at least partially to inhibition of adherent molecule expression and reduction of oxygen free radical formation during I/R pathophysiological processes and this protective action of MSODa was superior to SOD, highlighting the bright future for MSODa in clinical management of tissue I/R injury.

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Section: Effects Of Msoda On Plasma Mda Before and After Ischemia/repmentioning

confidence: 99%

Section: Effects Of Msoda Tissue Mpo Before and After Ischemia/reperfmentioning

confidence: 99%

Protective Effects of Modeled Superoxide Dismutase Coordination Compound (MSODa) Against Ischemia/Reperfusion Injury in Rat Skeletal Muscle

Wang¹,

Tian²,

Xu³

et al. 2015

Cell Physiol Biochem

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“…In this study, a rat model of limb ischaemia‐reperfusion was established to investigate the protective effect of H 2 S on limb IRI. When skeletal muscle tissue undergoes IRI, tissue necrotic markers, including MB, LPC, and LPO, increase because of necrosis or apoptosis of skeletal muscle cells . At the same time, MB, LPC, and LPO levels in the liver, lung, and kidney were detected and significantly increased after limb IRI in rats.…”

Section: Discussionmentioning

confidence: 95%

“…Limb ischaemia and reperfusion occurs when tissue is reperfused following a period of ischaemia, which is commonly encountered in surgical pathology, especially in peripheral vascular surgery that requires longer time and intraoperative blood flow . The process of lower limb IRI usually leads to degeneration, necrosis, or apoptosis of skeletal muscle tissue.…”

Section: Discussionmentioning

confidence: 99%

Effect of hydrogen sulphide on inflammatory factors of the mitochondria after limb ischaemia‐reperfusion injury in rats

Cheng

Zhang

2019

International Wound Journal

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The goal of this study was to evaluate the effect of hydrogen sulphide on inflammatory factors and the energy metabolism of mitochondria after limb reperfusion injury in rats. Sixty Wistar rats were divided into three groups: the sham operated group, the control group (the ischaemia‐reperfusion injury [IRI] + normal saline group), and the experimental group (the IRI + H2S group). An experimental rat model of limb IRI was established. Skeletal muscle samples were collected to observe the content of necrotic products (including myoglobin (MB), lysophosphatidylcholine (LPC), and lipid peroxidation (LPO)); blood samples were collected to observe changes in the contents of interleukin‐1 (IL‐1), Interleukin‐6 (IL‐6), and tumor necrosis factor‐α (TNF‐α); and the mitochondria of skeletal muscle cells were extracted for mitochondrial transmembrane potential measurement and adenosine triphosphate (ATP) content determination. The results underwent further statistical analysis. The contents of MB, LPC, and LPO in the limb skeletal muscle, liver, lung, and kidney tissues of rats in the control group were significantly increased (P < 0.05) after IRI, which was markedly attenuated by treatment with hydrogen sulphide (P < 0.05). Ischaemia/reperfusion of the lower extremities in rats triggered a significant increase in serum levels of IL‐1, IL‐6, and TNF‐α, which was significantly inhibited by treatment with H2S during ischaemia/reperfusion. In addition, the inhibitory effect tended to be time‐dependent. After limb ischaemia/reperfusion, the mitochondrial transmembrane potential of skeletal muscle cells in the control group decreased significantly (P < 0.05), while the potential energy of the mitochondrial membrane in the experimental group was significantly higher than that in the control group (P < 0.05). The content of ATP in mitochondria of skeletal muscle cells of ischaemia‐reperfusion rats in the control group was significantly lower than that in the sham operated group (P < 0.05), while the content of ATP of mitochondria in the experimental group after H2S treatment was significantly higher than the control group (P < 0.05). Hydrogen sulphide can alleviate the injury of skeletal muscle and distal organs after limb ischaemia‐reperfusion and reduce local inflammatory reaction, which is essential in alleviating mitochondrial transmembrane potential and energy metabolism disorder during reperfusion injury. The purpose of the study is to summarise the available information and provide theoretical support for the application of hydrogen sulphide in the treatment of limb IRI in skeletal muscle and distal organs.

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“…Skeletal muscle has high metabolic activity and therefore is sensitive to reperfusion injury after ischemia [4]. Despite rapid restoration of blood flow after severe ischemia, I/R injury causes continuous damage and necrosis of skeletal muscle, which may lead to amputation and multi-system organ dysfunction syndrome [5].…”

Section: Introductionmentioning

confidence: 99%

Protective Effects of Sonic Hedgehog Against Ischemia/Reperfusion Injury in Mouse Skeletal Muscle via AKT/mTOR/p70S6K Signaling

Zeng¹,

Fu²,

Wang³

et al. 2017

Cell Physiol Biochem

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Background/Aims: Skeletal muscle ischemia/reperfusion (I/R) injury is a common and severe disease. Sonic hedgehog (Shh) plays a critical role in post-natal skeletal muscle regeneration. In the present study, the role of Shh in skeletal muscle I/R injury and the mechanisms involved were investigated. Methods: The expression of Shh, AKT/mTOR/p70S6K and apoptosis pathway components were evaluated following tourniquet-induced skeletal muscle I/R injury. Then, mice were subjected to systemic administration of cyclopamine or one-shot treatment of a plasmid encoding the human Shh gene (phShh) to examine the effects of Shh on I/R injury. Moreover, mice were subjected to systemic administration of NVP-BEZ235 to investigate the role of the AKT/mTOR/p70S6K pathway in Shh-triggered skeletal muscle protection. Results: We found that the levels of Shh, AKT/mTOR/p70S6K pathway components and Cleaved Caspase 3 and the Bax/Bcl2 ratio initially increased and then decreased at different time points post-I/R injury. Moreover, Shh protected skeletal muscle against I/R injury by alleviating muscle destruction, reducing interstitial fibrosis and inhibiting apoptosis, and these protective effects were abrogated when the AKT/mTOR/p70S6K pathway was inhibited. Conclusion: Collectively, these data suggest that Shh signaling exerts a protective role through the AKT/mTOR/p70S6K signaling pathway during skeletal muscle I/R injury. Thus, Shh signaling may be a therapeutic target for protecting skeletal muscle from I/R injury.

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Hydrogen-rich saline attenuates ischemia–reperfusion injury in skeletal muscle

Cited by 36 publications

References 51 publications

Protective Effects of Modeled Superoxide Dismutase Coordination Compound (MSODa) Against Ischemia/Reperfusion Injury in Rat Skeletal Muscle

Protective Effects of Modeled Superoxide Dismutase Coordination Compound (MSODa) Against Ischemia/Reperfusion Injury in Rat Skeletal Muscle

Effect of hydrogen sulphide on inflammatory factors of the mitochondria after limb ischaemia‐reperfusion injury in rats

Protective Effects of Sonic Hedgehog Against Ischemia/Reperfusion Injury in Mouse Skeletal Muscle via AKT/mTOR/p70S6K Signaling

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