Protective Effects of Modeled Superoxide Dismutase Coordination Compound (MSODa) Against Ischemia/Reperfusion Injury in Rat Skeletal Muscle

Wang, Xintao; Tian, Ye; Xu, Wenchang; Cui, Li-Huang; Xiang, Shou-Yang; Lü, Song-Cen

doi:10.1159/000430369

Cited by 18 publications

(9 citation statements)

References 36 publications

(32 reference statements)

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“…Similar results were reported previously where the MDA content was significantly increased in rat triceps after exhaustive downhill run, which could remarkably be decreased by the delivery of vitamin E, one product against lipid peroxidation caused by the oxygen free radical [38]. …”

Section: Discussionsupporting

confidence: 89%

Effects of intermittent pressure imitating rolling manipulation on calcium ion homeostasis in human skeletal muscle cells

Zhang

Liu

Lin

et al. 2016

BMC Complement Altern Med

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BackgroundHomeostasis imbalance of intracellular Ca2+ is one of the key pathophysiological factors in skeletal muscle injuries. Such imbalance can cause significant change in the metabolism of Ca2+-related biomarkers in skeletal muscle, such as superoxide dismutase (SOD), malondialdehyde (MDA) and creatine kinase (CK). Measurements of these biomarkers can be used to evaluate the degree of damage to human skeletal muscle cells (HSKMCs) injury. Rolling manipulation is the most popular myofascial release technique in Traditional Chinese Medicine. The mechanism of how this technique works in ameliorating muscle injury is unknown. This study aimed to investigate the possible Ca2+ mediated effects of intermittent pressure imitating rolling manipulation (IPIRM) of Traditional Chinese Medicine in the injured HSKMCs.MethodsThe normal HSKMCs was used as control normal group (CNG), while the injured HSKMCs were further divided into five different groups: control injured group (CIG), Rolling manipulation group (RMG), Rolling manipulation-Verapamil group (RMVG), static pressure group (SPG) and static pressure-Verapamil group (SPVG). RMG and RMVG cells were cyclically exposed to 9.5-12.5 N/cm2 of IPIRM at a frequency of 1.0 Hz for 10 min. SPG and SPVG were loaded to a continuous pressure of 12.5 N/cm2 for 10 min. Verapamil, a calcium antagonist, was added into the culture mediums of both RMVG and SPVG groups to block the influx of calcium ion.ResultCompared with the CNG (normal cells), SOD activity was remarkably decreased while both MDA content and CK activity were significantly increased in the CIG (injured cells). When the injured cells were treated with the intermittent rolling manipulation pressure (RMG), the SOD activity was significantly increased and MDA content and CK activity were remarkably decreased. These effects were suppressed by adding the calcium antagonist Verapamil into the culture medium in RMVG. On the other hand, exposure to static pressure in SPG and SPVG affected neither the SOD activity nor the MDA content and CK activity in the injured muscle cells regardless of the presence of verapamil or not in the culture medium.ConclusionThese data suggest that the intermittent rolling pressure with the manipulation could ameliorate HSKMCs injury through a Ca2+ dependent pathway. Static pressure did not lead to the same results.

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Section: Discussionsupporting

confidence: 89%

Effects of intermittent pressure imitating rolling manipulation on calcium ion homeostasis in human skeletal muscle cells

Zhang

Liu

Lin

et al. 2016

BMC Complement Altern Med

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“…Moreover, enlarged mitochondria with loss of cristae were detected. This is in accordance with Wang et al, 2015 [19] who mentioned muscular dissolution and mitochondrial swelling in IR injury of skeletal muscles.…”

Section: Discussionsupporting

confidence: 92%

A histological Study Comparing the Possible Protective Mechanisms of Oxytocin and Vitamin E on ischemia reperfusion injury in Rat skeletal muscle

Nada

El-Deeb

Aboulkhair

et al. 2018

Egyptian Journal of Histology

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Background: Ischemia-reperfusion (IR) injury in skeletal muscle is a pathophysiology which affect quality of life. Aim of Work: To compare the possible protective mechanisms by which oxytocin and Vitamin E may exert on skeletal muscle IR injury in rat. Materials and Methods: 28 male rats were divided into: Control (Group A) of 10 rats and IR group (Group B) of 18 rats. Control group underwent the same surgical steps as IR group but without clamping the femoral artery followed by the injection of one of the following [saline (AI), oxytocin (AII) or vitamin E (AIII)]. In the IR group, lower limb ischemia was induced by clamping the femoral artery. After 4h of ischemia, the clamp was removed to allow reperfusion coinciding with the injection of one of the following [saline (BI), Oxytocin (0.5µg/kg) (BII) and Vitamin E (10mg/kg) (BIII)]. After 2 hours of reperfusion, Blood samples were drawn from the femoral vein to measure glutathione (GSH) and malondialdehyde (MDA). Gastrocnemius muscles were obtained for evaluation by light and electron microscopes. Cytochrome C, Myogenin immunohistochemistry and statistical analysis were applied. Results: A significant increase in GSH and significant decrease in MDA were reported in BII and BIII as compared to BI. Mean area % of cytochrome C in BII and BIII showed a significant decrease compared to BI. Mean number of myogenin immunopositive cells in BII was significantly higher compared to BI. Muscular damage was proved by light and electron microscopic findings in BI. While, BII and BIII encountered evident protection from muscular damage induced by IR injury. Conclusion: Oxytocin exerts weak protective antioxidant effect against IR injury. However, it activates stem cells to regenerate the damaged muscle. Vitamin E was better antioxidant which can react as early as 1 hour in the protection of the skeletal muscle against IR injury.

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“…The role of oxidative stress in the setting of hind limb I/R injury is well demonstrated in previous studies [27–29]. Hence, we investigated the effects of ScB on the level of MDA and SOD activity.…”

Section: Discussionmentioning

confidence: 79%

Schisandrin B Prevents Hind Limb from Ischemia-Reperfusion-Induced Oxidative Stress and Inflammation via MAPK/NF-κB Pathways in Rats

Zhu

Cai

Zhou

et al. 2017

BioMed Research International

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Schisandrin B (ScB), isolated from Schisandra chinensis (S. chinensis), is a traditional Chinese medicine with proven cardioprotective and neuroprotective effects. However, it is unclear whether ScB also has beneficial effects on rat hind limb ischemia/reperfusion (I/R) injury model. In this study, ScB (20 mg/kg, 40 mg/kg, and 80 mg/kg) was administered via oral gavage once daily for 5 days before the surgery. After 6 h ischemia and 24 h reperfusion of left hind limb, ScB reduced I/R induced histological changes and edema. ScB also suppressed the oxidative stress through decreasing MDA level and increasing SOD activity. Moreover, above changes were associated with downregulated TNF-α mRNA expression and reduced level of IL-1β in plasma. Meanwhile, ScB treatment downregulated activation of p38MAPK, ERK1/2, and NF-κB in ischemic skeletal muscle. These results demonstrate that ScB treatment could prevent hind limb I/R skeletal muscle injury possibly by attenuating oxidative stress and inflammation via p38MAPK, ERK1/2, and NF-κB pathways.

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Protective Effects of Modeled Superoxide Dismutase Coordination Compound (MSODa) Against Ischemia/Reperfusion Injury in Rat Skeletal Muscle

Cited by 18 publications

References 36 publications

Effects of intermittent pressure imitating rolling manipulation on calcium ion homeostasis in human skeletal muscle cells

Effects of intermittent pressure imitating rolling manipulation on calcium ion homeostasis in human skeletal muscle cells

A histological Study Comparing the Possible Protective Mechanisms of Oxytocin and Vitamin E on ischemia reperfusion injury in Rat skeletal muscle

Schisandrin B Prevents Hind Limb from Ischemia-Reperfusion-Induced Oxidative Stress and Inflammation via MAPK/NF-κB Pathways in Rats

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