Hydrogen production by immobilized Synechocystis sp. PCC 6803

Touloupakis, Eleftherios; Rontogiannis, George; Benavides, Ana Margarita Silva; Cicchi, Bernardo; Ghanotakis, Demetrios F.; Torzillo, Giuseppe

doi:10.1016/j.ijhydene.2016.07.075

Cited by 63 publications

(16 citation statements)

References 33 publications

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“…Indeed, Gosse et al 11,13 have recently demonstrated that the entrapment of the phototrophic bacterium, Rhodopseudomonas palustris within latex coatings extends the duration of H 2 photoproduction leading to increased H 2 -production yields compared to suspension cultures. A similar effect has been demonstrated for H 2 -producing green algae 14,15 and heterocystous cyanobacteria 16,17 entrapped within thin Ca 2+ -alginate hydrogel cubes, beads and lms. The improvement of photosynthetic productivity was also conrmed for a few species of cyanobacteria entrapped in the hydrated latex coatings.…”

Section: Introductionsupporting

confidence: 70%

Versatile templates from cellulose nanofibrils for photosynthetic microbial biofuel production

et al. 2018

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Versatile templates were fabricated using plant-derived nanomaterials, TEMPO-oxidized cellulose nanofibrils (TEMPO CNF) for the efficient and sustainable production of biofuels from cyanobacteria and green algae. We used three different approaches to immobilize the model filamentous cyanobacteria or green algae to the TEMPO CNF matrix. These approaches involved the fabrication of: (A) a pure TEMPO CNF hydrogel; (B) a Ca 2+ -stabilized TEMPO CNF hydrogel; and (C) a solid TEMPO CNF film, which was crosslinked with polyvinyl alcohol (PVA). The different immobilization approaches resulted in matrices with enhanced water stability performance. In all cases, the photosynthetic activity and H 2 photoproduction capacity of cyanobacteria and algae entrapped in TEMPO CNF were comparable to a conventional alginate-based matrix. Green algae entrapped in Ca 2+ -stabilized TEMPO CNF hydrogels showed even greater rates of H 2 production than control alginate-entrapped algae under the more challenging submerged cultivation condition. Importantly, cyanobacterial filaments entrapped within dried TEMPO CNF films showed full recovery once rewetted, and they continued efficient H 2 production. The immobilization mechanism was passive entrapment, which was directly evidenced using surface sensitive quartz crystal microbalance with dissipation monitoring (QCM-D). The results obtained demonstrate a high compatibility between CNF and photosynthetic microbes. This opens new possibilities for developing a novel technology platform based on CNF templates with tailored pore-size and controllable surface charges that target sustainable chemical production by oxygenic photosynthetic microorganisms.

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Section: Introductionsupporting

confidence: 70%

Versatile templates from cellulose nanofibrils for photosynthetic microbial biofuel production

et al. 2018

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“…The cyanobacteria Synechocystis sp. PCC 6803 in silica matrices showed that hydrogen production of encapsulated cells was higher than that of cells in suspension [ 53 , 54 ]. Despite these advances, hydrogen production efficiency using biological organisms has been limited by its low volumetric productivity.…”

Section: Resultsmentioning

confidence: 99%

Diatom-inspired multiscale mineralization of patterned protein–polysaccharide complex structures

Wang

et al. 2020

National Science Review

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Marine diatoms construct their hierarchically ordered, three-dimensional (3D) external structures called frustules through precise biomineralization processes. Recapitulating the remarkable architectures and functions of diatom frustules in artificial materials is a major challenge that has important technological implications for hierarchically ordered composites. Here, we report the construction of highly ordered, mineralized composites based on fabrication of complex self-supporting porous structures—made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin—and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2, and Ga2O3. Subsequently, using sugar cubes as templates, we demonstrate that 3D fabricated porous structures can become colonized by engineered bacteria and can be functionalized with highly photoreactive minerals, thereby enabling co-localization of the photocatalytic units with a bacteria-based hydrogenase reaction for a successful semi-solid artificial photosynthesis system for hydrogen evolution. Our study thus highlights the power of coupling genetically engineered proteins and polysaccharides with biofabrication techniques to generate hierarchically organized mineralized porous structures inspired by nature.

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“…Alginate hydrogel cultivation with this harvesting scheme also applies to biofuel‐relevant microalgae species, including C. vulgaris , Chlorella sorokiniana , Scenedesmus obliquu s, Botryococcus braunii , Anabaena PCC 7120, and Synechocystis sp. PCC 6803 (Bailliez et al, ; De‐Bashan et al, ; Leino et al, ; Mallick, ; Touloupakis et al, ). These results point to an opportunity for greater productivity via optimizing species selection, loading density, harvest layer thickness, alginate composition, and cross‐linker concentration.…”

Section: Discussionmentioning

confidence: 99%

Periodic harvesting of microalgae from calcium alginate hydrogels for sustained high‐density production

Pierobon

Riordon

Nguyen

et al. 2017

Biotech & Bioengineering

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High-density biomass production is currently only realized in biofilm-based photobioreactors. Harvest yields of whole biofilms are self-limited by daughter-upon-parent cell growth that hinders light and leads to respiratory biomass losses. In this work, we demonstrate a sustainable multi-harvest approach for prolonged generation of high-density biomass. Calcium-alginate hydrogel cultures loaded with Synechococcus elongatus PCC 7942 achieved production densities comparable to that of biofilms (10 cells/mL) and optimal total productivity in harvest periods of 2 or 3 days that allowed high-density surface growth without self-limiting cell buildup or surface death. Cross-linking calcium concentration had a strong influence on surface growth and harvest yields, especially in the first harvests. Subsequent harvests achieved more uniform biomass yields and distributions, unaffected by bulk respiration or light penetration. Collectively, these results demonstrate the feasibility of sustained, high-density biomass production by periodic harvesting within microalgal hydrogel cultures. Biotechnol. Bioeng. 2017;114: 2023-2031. © 2017 Wiley Periodicals, Inc.

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Hydrogen production by immobilized Synechocystis sp. PCC 6803

Cited by 63 publications

References 33 publications

Versatile templates from cellulose nanofibrils for photosynthetic microbial biofuel production

Versatile templates from cellulose nanofibrils for photosynthetic microbial biofuel production

Diatom-inspired multiscale mineralization of patterned protein–polysaccharide complex structures

Periodic harvesting of microalgae from calcium alginate hydrogels for sustained high‐density production

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