Hydrogen Peroxide Production and Free Radical-mediated Cell Stress in Mycoplasma bovis Pneumonia

Schott, Courtney R.; Cai, Hugh Y.; Parker, Lois; Bateman, K G; Caswell, Jeff

doi:10.1016/j.jcpa.2013.07.008

Cited by 30 publications

(26 citation statements)

References 28 publications

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“…In calves with Mycoplasma bronchopneumonia, there was a significant alteration in major and trace elements in the bronchoalveolar lavage fluid [17]. In cattle, the oxidative stress level and antioxidant status have been described in bronchopneumonia caused by Mycoplasma bovis [18,19]. The camel, however, has been neglected for a long time and reports on pasteurellosis and their associated oxidant/antioxidant imbalance are scare.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Trace Elements and Oxidative Stress Levels Associated with Pasteurellosis in Camel-Calves (Camelus dromedarius)

Shoieb¹,

Ibrahim²,

Sayed-Ahmed³

et al. 2016

J Vet Sci Technol

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Section: Introductionmentioning

confidence: 99%

Antioxidant Trace Elements and Oxidative Stress Levels Associated with Pasteurellosis in Camel-Calves (Camelus dromedarius)

Shoieb¹,

Ibrahim²,

Sayed-Ahmed³

et al. 2016

J Vet Sci Technol

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“…Mycoplasmal membrane is important in colonization and establishment of infection, as the organism lacks cell wall (Rottem, 2003). Other pathogenic mechanisms include production of hydrogen peroxide and superoxide radicals (Schott et al, 2014). Moreover, membrane lipoproteins, which undergo phase and size variation aid in immune evasion (Rosengarten et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Effect of Mycoplasma bovis on Production of Pro-Inflammatory Cytokines by Peripheral Blood Mononuclear Cells

Valsala¹,

Rana²,

Remesh³

et al. 2017

Adv. Anim. Vet. Sci.

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| Mycoplasma bovis is an important bovine pathogen associated with calf pneumonia, mastitis, arthritis and sepicemia. It is known to persist in phagocytic and nonphagocytic cells and disseminate to various organs causing systemic infection. In this study, the effect of live M. bovis on pro-inflammatory cytokine production by bovine peripheral blood mononuclear cells was assessed by relative quantification. Temporal change in cytokine levels was assessed at 8 hourly intervals. IL-6 was upregulated (9.32 fold at 24h) by live M. bovis at MOI of 1: 10, while TNF-α levels remained unchanged over time. IL-1 and TNF-α levels were not altered by M. bovis as compared to the control group. This showed that pro-inflammatory cytokines are not much upregulated at low multiplicity of infection.

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“…The production of phospholipases, hydrogen peroxide and superoxide radicals can induce toxic damage to the host cells (Khan et al, 2005b, Maunsell et al, 2011, Quinn et al, 2011, Schott et al, 2014. Khan et al (2005a) showed that the ability of 16 M. bovis strains from the UK (14 bovine field isolates, one human isolate and the type strain NCTC 10131), varied widely in their ability to produce hydrogen peroxide.…”

Section: Toxic Metabolite Productionmentioning

confidence: 99%

“…Mycoplasma bovis also has the ability to produce hydrogen peroxide (H 2 O 2 ) which has been suggested as an important virulence factor causing oxidative injury to lung tissue (Khan et al, 2005b, Schott et al, 2013. Furthermore, Schott et al (2013) speculate that the generation of oxygen free radicals at the sites of M. bovis infection can be detrimental to the local immune response and contribute to the chronicity that is typical of M. bovis infections.…”

Section: Brd50 and Sero-conversion To M Bovis Or A Sero-increase In mentioning

confidence: 99%

“…Furthermore, Schott et al (2013) speculate that the generation of oxygen free radicals at the sites of M. bovis infection can be detrimental to the local immune response and contribute to the chronicity that is typical of M. bovis infections. Any one or combination of these factors may lead to suppression or down regulation of the local immune response allowing other bacteria to invade tissue in the LRT.…”

Section: Brd50 and Sero-conversion To M Bovis Or A Sero-increase In mentioning

confidence: 99%

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Mycoplasma bovis in Australian feeder cattle

Schibrowski¹

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Mycoplasma bovis can be a commensal bacterial inhabitant of the upper respiratory tract of healthy bovines. However, under certain circumstances and conditions that are incompletely understood, M. bovis can be associated with a number of clinical syndromes, one of which is bovine respiratory disease (BRD). In Australia, there is little information on the prevalence and epidemiology M. bovis in beef production systems. The overall aim of this thesis was to help close this knowledge gap.Commercially available serological diagnostic methods for the detection of M. bovis-specific antibody have not been validated in the Australian cattle population. Arguably, more importantly, however is the fact that diagnostic sensitivity (Se) and specificity (Sp) and / or analytical sensitivity (ASe) and specificity (ASp) estimates for commercially available M. bovis sero-diagnostic tests are not available. 28% (95% CI: 1% -92%). However, in terms of Sp the commercial ELISAs out performed western blotting; BioK302: 96% (95% CI: 87% -99%); BioK260:100% (95% CI: 93% -100%) and western blotting: 88% (95% CI: 56% -98%) respectively. In terms of both Se and Sp combined, none of the methods assessed here performed well. As such, when interpreting the results obtained from these ELISAs, the potential impact imperfect Se and / or Sp can have needs to be considered.Despite the poor performance of the BioK302 and BioK260 ELISAs particularly in terms of Se, these tests were used to determine the sero-prevalence of M. bovis antibody in Australian feeder cattle at feedlot induction and draft (approximately 42 days on feed). Paired bovine sera from 1,354 randomly selected animals were sourced from a bovine serum bank (n = 35,160) collected as part of another study, The National Bovine Respiratory Disease Initiative. As the classification of the result of a single serum sample could differ between the BioK302 and BioK260, sero-prevalence estimates were obtained using the individual ELISA results in addition to series and parallel interpretation of the two ELISA kit results. The apparent sero-prevalence of M. bovis-specific antibody at feedlot induction estimates ranged from 2.4% -6.8% (95% CI: 1.3% -8.6%) and the apparent sero-prevalence estimates at draft ranged from 22.4% -44.2% (95% CI: 19.1% -48.6%). The level of sero-conversion to M. bovis between induction and draft was also determined. Only animals that were classified as sero-negative at induction were included in sero-conversion analyses. In the current study, 24.3% (95% iii CI: 20.8% -27.7%) and 19.5% (95% CI: 17.3% -21.7%) of animals sero-converted to M. bovis between induction and draft based on the BioK302 and dichotomised BioK260 ELISA results respectively.Using these ELISA results, an assessment of putative risk factors associated with sero-positivity at feedlot induction or sero-conversion between induction and draft was performed. An increased risk of sero-positivity at feedlot induction was found to be associated with; (i) history of exposure to a saleyard at least 27 d...

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Hydrogen Peroxide Production and Free Radical-mediated Cell Stress in Mycoplasma bovis Pneumonia

Cited by 30 publications

References 28 publications

Antioxidant Trace Elements and Oxidative Stress Levels Associated with Pasteurellosis in Camel-Calves (Camelus dromedarius)

Antioxidant Trace Elements and Oxidative Stress Levels Associated with Pasteurellosis in Camel-Calves (Camelus dromedarius)

Effect of Mycoplasma bovis on Production of Pro-Inflammatory Cytokines by Peripheral Blood Mononuclear Cells

Mycoplasma bovis in Australian feeder cattle

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