Hydrogen peroxide might be a downstream signal molecule of hydrogen sulfide in seed germination of mung bean (Vigna radiata)

Abstract

Phenylalanine ammonia-lyase (PAL) and cinnamate 4-hydroxylase (C4H) are the first and second key enzymes of the phenylpropanoid pathway. Systematic analysis of the DfPAL gene family and DfC4H have not been performed in Dryopteris fragrans (L.) Schott. To date, PAL and C4H genes have been less extensively studied in monilophytes than in angiosperms. Here we report the identification of threeDfPAL and DfC4H fragments using cDNA cloning and sequencing. Bioinformatics and phylogenetic analyses showed that DfPAL1 and DfPAL2 were quite similar at the amino acid level (94.88%), whereas DfPAL3 was relatively low similar to both of the other paralogs. Some important functional domains were conserved in three DfPAL and DfC4H genes. DfPAL3 and DfC4H were highly expressed in gametophytes and petioles of D. fragrans, DfPAL1 had the highest expression in petioles, and DfPAL2 had low expression in leaves and petioles. Only DfPAL2 and DfC4H were induced with 4 • C, 35 • C, and UV treatments, but the time responses were different. These results suggest complexity of the DfPAL-and DfC4H-associated metabolic network in D. fragrans. The results provide a basis for elucidating the role of DfPAL and DfC4H genes in the biosynthesis of bioactive compounds.

Improving Photosynthetic Capacity, Alleviating Photosynthetic Inhibition and Oxidative Stress Under Low Temperature Stress With Exogenous Hydrogen Sulfide in Blueberry Seedlings

“…Under lead stress, H 2 S and NO can improve the antioxidant system and mineral balance of sesame by interacting (Amooaghaie et al, 2017). H 2 S can be used as upstream signaling molecule of H 2 O 2 to promote mung bean (Vigna radiata) seed germination (Li and He, 2015). H 2 S can be used as a signal molecule of salicylic acid (SA) to participate in Cd tolerance in Arabidopsis thaliana (Qiao et al, 2015).…”

Hydrogen Sulfide: A Signal Molecule in Plant Cross-Adaptation

“…The accumulation of endogenous H 2 S has become a common response of plants to environmental stress, including salt, heavy metal (HM), drought, heat and cold stress, as well as pathogen infection, which may be closely associated with the acquisition of stress tolerance in plants (Li, 2013; Calderwood and Kopriva, 2014; Hancock and Whiteman, 2014). More interestingly, exogenously applied H 2 S, releasing from its donors such as NaHS and morpholin-4-ium 4-methoxyphenyl(morpholino) phosphinodithioate (GYY4137), shows significant positive effects on seed germination (Li et al, 2012a; Li and He, 2015; Wojtyla et al, 2016), organogenesis and growth (Lin et al, 2012; Fang T. et al, 2014), the regulation of senescence (Zhang et al, 2011), as well as the acquisition of stress tolerance such as salt (Christou et al, 2013), HM (Chen et al, 2013), drought (Christou et al, 2013), heat (Li et al, 2013a,b; Li, 2015c) and cold tolerance (Fu et al, 2013). These results indicate that H 2 S may be a candidate signal molecule in plant cross-adaptation.…”

Genomics: A Hallmark to Monitor Molecular and Biochemical Processes Leading Toward a Better Perceptive of Seed Aging and ex-situ Conservation

“…Damage the components of cell which leads to deterioration of oil seeds Shelar et al, 2008;Li and He, 2015 Chromosome Aberrations Damage in chromosomal structure leads to expression alterations.…”

“…During the storage of dry seed, non-enzymatic mechanisms, such as lipid peroxidation, are thought to be responsible for the accumulation of ROS, owing to the absence of free water, whereas enzymatic mechanisms are responsible for ROS production during imbibition. However, in seeds with dynamic metabolic activities, the mitochondrial respiratory chain is thought to be the main source of ROS generation (Bailly, 2004), and the deposition of ROS can also be accelerated by alterations in mitochondria (Wei and Lee, 2002;Cash et al, 2007;Li et al, 2015).…”

“…In sunflower seeds, reductions in seed viability and vigor that occur during aging are associated with accumulation of hydrogen peroxide, accelerated lipid peroxidation, and reduced activity (Kibinza et al, 2006;Nagel et al, 2009;Li et al, 2015). Metallothioneins (MTs) serve as signaling molecules and ROS scavengers, both within and outside the nucleus, , which highlights their potential interaction with DNA repair machinery .…”