Hydrogen peroxide induces protection against N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) effects in Escherichia coli

Asad, L.M.B.O.; Asad, Nasser Ribeiro; Silva, André B.; Felzenszwalb, Israel; Leitão, Álvaro C.

doi:10.1016/s0921-8777(96)00053-5

Cited by 17 publications

(7 citation statements)

References 20 publications

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“…Earlier such response negated subsequent toxicity induced by a variety of aldehyde compounds in E. coli [25]. Similarly H 2 O 2 pre-treatment also protected E. coli cells against methylnitronitrosoguanidine induced lethality which is known to progress by ROS generation [26]. But in the current investigation the adaptive oxidative stress response did not aid E. coli cells in combating a subsequent resveratrol challenge.…”

Section: Discussioncontrasting

confidence: 54%

Resveratrol induced inhibition of Escherichia coli proceeds via membrane oxidation and independent of diffusible reactive oxygen species generation

et al. 2014

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Resveratrol (5-[(E)-2-(4-hydroxyphenyl)ethenyl]benzene-1,3-diol), a redox active phytoalexin with a large number of beneficial activities is also known for antibacterial property. However the mechanism of action of resveratrol against bacteria remains unknown. Due to its extensive redox property it was envisaged if reactive oxygen species (ROS) generation by resveratrol could be a reason behind its antibacterial activity. Employing Escherichia coli as a model organism we have evaluated the role of diffusible reactive oxygen species in the events leading to inhibition of this organism by resveratrol. Evidence for the role of ROS in E. coli treated with resveratrol was investigated by direct quantification of ROS by flow cytometry, supplementation with ROS scavengers, depletion of intracellular glutathione, employing mutants devoid of enzymatic antioxidant defences, induction of adaptive response prior to resveratrol challenge and monitoring oxidative stress response elements oxyR, soxS and soxR upon resveratrol treatment. Resveratrol treatment did not result in scavengable ROS generation in E. coli cells. However, evidence towards membrane damage was obtained by potassium leakage (atomic absorption spectrometry) and propidium iodide uptake (flow cytometry and microscopy) as an early event. Based on the comprehensive evidences this study concludes for the first time the antibacterial property of resveratrol against E. coli does not progress via the diffusible ROS but is mediated by site-specific oxidative damage to the cell membrane as the primary event.

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Section: Discussioncontrasting

confidence: 54%

Resveratrol induced inhibition of Escherichia coli proceeds via membrane oxidation and independent of diffusible reactive oxygen species generation

et al. 2014

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“…Venkat et al (2001) observed a maximum AR when a test dose of 100 cGy was given four hours after an adaptive dose, 30 h following the mitogenic stimulation of lymphocytes. The Dimova et al 401 protective effect against N-methyl-N'-nitro-N-nitrosoguanidine induced by prior treatment with H 2 O 2 in E. coli is also time dependent, decreasing 15 min after the pretreatment and almost abolished after 30 min (Asad et al, 1997).…”

Section: Kinetics Of the Adaptive Responsementioning

confidence: 93%

“…The AR could be considered a nonspecific phenomenon -the exposure to minimal stress inducing a very low level of damage can trigger an AR resulting in increased resistance to higher levels of the same or of other types of stress (Joiner et al, 1996;Wolff, 1998;Joiner et al, 1999;Patra et al, 2003;Asad et al, 2004;Girigoswami and Ghosh, 2005;Yan et al, 2006). The AR has been observed in many different organisms: bacteria, yeast, the algae Oedogonium cardiacam, Chlamydomonas reinhardtii, Closterium monoliferum and Chlorella pyrenoidosa, in higher plants, insect cells, mammalian cells, human cells in vitro, and in animal models in vivo during a protracted (low dose-rate) exposure prior to an acute dose treatment Laszlo, 1971, 1973;Bryant, 1974Bryant, , 1975Bryant, , 1976Bryant, , 1979Cowie, 1976, 1978;Olivieri et al, 1984;Santier et al, 1985;Wolff et al, 1988;Boreham and Mitchel, 1991;Rieger et al, 1993;Mahmood et al, 1996;Salone et al, 1996;Panda et al, 1997;Asad et al, 1997Asad et al, , 1998Wolff, 1998;Nikolova et al,showing gene expression changes, DNA single-and double-strand breaks, biochemical analyses of enzymatic and/or non-enzymatic antioxidant defence system (Hillova and Drasil 1967;Bryant, 1975Bryant, , 1976Bryant, , 1979Rieger et al, 1993;Ikushima et al, 1996;Rigaud and Moustacchi, 1996;Panda et al, 1997;Nikolova et al, 1999;Robson et al, 2000;…”

Section: The Adaptive Responsementioning

confidence: 99%

Adaptive response: some underlying mechanisms and open questions

2008

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Organisms are affected by different DNA damaging agents naturally present in the environment or released as a result of human activity. Many defense mechanisms have evolved in organisms to minimize genotoxic damage. One of them is induced radioresistance or adaptive response. The adaptive response could be considered as a nonspecific phenomenon in which exposure to minimal stress could result in increased resistance to higher levels of the same or to other types of stress some hours later. A better understanding of the molecular mechanism underlying the adaptive response may lead to an improvement of cancer treatment, risk assessment and risk management strategies, radiation protection, e.g. of astronauts during long-term space flights. In this mini-review we discuss some open questions and the probable underlying mechanisms involved in adaptive response: the transcription of many genes and the activation of numerous signaling pathways that trigger cell defenses -DNA repair systems, induction of proteins synthesis, enhanced detoxification of free radicals and antioxidant production.

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“…Oxygen is an excellent electron acceptor and can be reduced to water or to hydrogen peroxide with or without the cross‐reaction with methyl viologen cation radicals at the applied cathodic potentials. High hydrogen peroxide formation and related cell damage could explain the stagnation of product formation . Controls regarding hydrogen peroxide formation showed minimal hydrogen peroxide accumulation in both H‐cells and 1 L electrobioreactors (maximum 0.08 m m and 0.06 m m , respectively).…”

Section: Resultsmentioning

confidence: 99%

Response‐Surface‐Optimized and Scaled‐Up Microbial Electrosynthesis of Chiral Alcohols

et al. 2020

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A variety of enzymes can be easily incorporated and overexpressed within Escherichia coli cells by plasmids, making it an ideal chassis for bioelectrosynthesis. It has recently been demonstrated that microbial electrosynthesis (MES) of chiral alcohols is possible by using genetically modified E. coli with plasmid‐incorporated and overexpressed enzymes and methyl viologen as mediator for electron transfer. This model system, using NADPH‐dependent alcohol dehydrogenase from Lactobacillus brevis to convert acetophenone into (R)‐1‐phenylethanol, is assessed by using a design of experiment (DoE) approach. Process optimization is achieved with a 2.4‐fold increased yield of 94±7 %, a 3.9‐fold increased reaction rate of 324±67 μm h−1, and a coulombic efficiency of up to 68±7 %, while maintaining an excellent enantioselectivity of >99 %. Subsequent scale‐up to 1 L by using electrobioreactors under batch and fed‐batch conditions increases the titer of (R)‐1‐phenylethanol to 12.8±2.0 mm and paves the way to further develop E. coli into a universal chassis for MES in a standard biotechnological process environment.

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Hydrogen peroxide induces protection against N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) effects in Escherichia coli

Cited by 17 publications

References 20 publications

Resveratrol induced inhibition of Escherichia coli proceeds via membrane oxidation and independent of diffusible reactive oxygen species generation

Resveratrol induced inhibition of Escherichia coli proceeds via membrane oxidation and independent of diffusible reactive oxygen species generation

Adaptive response: some underlying mechanisms and open questions

Response‐Surface‐Optimized and Scaled‐Up Microbial Electrosynthesis of Chiral Alcohols

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