Hydrogen peroxide-induced oxidative stress activates NF-κB and Nrf2/Keap1 signals and triggers autophagy in piglets

Yin, Jie; Duan, Jielin; Cui, Zhijie; Ren, Wenkai; Li, Tiejun; Yin, Yulong

doi:10.1039/c4ra13557a

Cited by 113 publications

(106 citation statements)

References 35 publications

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“…This finding was consistent with our results. Even though we did not record feed intake in the weaning group, the reduction in body weight was mainly attributed to low feed intakes (Hampson and Kidder 1986;Appleby et al 1991;Fraser et al 1994;Bruininx et al 2002;Boudry et al 2004;Yin et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Transient effects of weaning on the health of newly weaning piglets

Tao¹,

Xu²,

Men³

2016

CZECH J ANIM SCI

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ABSTRACT:The transient effects of weaning on the health of newly weaning piglets were investigated. In this study, 24 piglets were randomly assigned to weaning and suckling (control) groups. Weaning piglets were sacrificed on days 1, 4, and 7 post-weaning; suckling piglets were slaughtered at the corresponding time points. Blood samples and internal organs were collected for the determination of hematological traits, relative organ weights, and serum biochemical, antioxidant, enzyme, and immune indexes. The results revealed that piglets' growth performance significantly decreased on days 4 and 7 post-weaning. White blood cell and lymphocyte counts changed on days 1 and 4 post-weaning. On day 4, serum glucose levels decreased and urea nitrogen levels increased. Thymus gland weight significantly decreased on day 7, while adrenal gland weight significantly increased on days 4 and 7. Malondialdehyde level increased on day 4 post-weaning and superoxide dismutase, catalase, and alkaline phosphatase activities decreased on days 4 and 7 post-weaning. Serum IgA concentrations decreased from day 1 to day 4 post-weaning. The results showed that the adverse effects of weaning on the serum biochemical indexes gradually weakened on day 4 post-weaning. However, during the first week postweaning, there were damaging effects on the endocrine, antioxidant, intestinal, and immune systems of piglets.Keywords: hematological traits; serum biochemical indexes; enzyme; immune List of abbreviations: W1d, W4d, W7d = weaning piglets slaughtered on days 1, 4, and 7 post-weaning, respectively, S1d, S4d, S7d = suckling piglets slaughtered on days 1, 4, and 7, respectively, ADG = average daily gain, WBC = white blood cell count, GRAN = neutrophilic granulocyte count, LYMF = lymphocyte count, MONO = monocyte count, GR = granulocyte percentage, LY = lymphocyte percentage, MO = monocyte percentage, RBC = red blood cell, HGB = hemoglobin, HCT = hematocrit, MCV = mean corpuscular volume, MCH = mean corpuscular hemoglobin, MCHC = mean corpuscular hemoglobin concentration, RDW = red blood cell volume distribution width, PLT = blood platelet counts, PCT = platelet trait, MPV = mean platelet volume, PDW = platelet distribution width, TP = total protein, ALB = albumin, Glu = glucose, BUN = serum urea nitrogen, TCH = total cholesterol, LDL = lowdensity lipoprotein, MDA = malondialdehyde, T-AOC = total antioxidant capacity, SOD = superoxide dismutase, CAT = catalase, XOD = xanthine oxidase, CP = ceruloplasmin, AKP = alkaline phosphatase, 5'-NT = 5'nucleotidase

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Section: Discussionmentioning

confidence: 99%

Transient effects of weaning on the health of newly weaning piglets

Tao¹,

Xu²,

Men³

2016

CZECH J ANIM SCI

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“…It is widely confirmed that NF-κB signaling pathway plays a key role in inflammatory response and mediates various proinflammatory cytokines generation in different pathologies (Nguyen et al 2014;Ruan and Chen 2012;Yin et al 2015a). Symeonidou et al investigated 128 genes' expression profiles from NF-κB signaling pathway in Trichinella spiralis-infected mice via microarray analysis and found that various gene expressions were affected between the specific points of Trichinella spiralis infection (Symeonidou et al 2010).…”

Section: Discussionmentioning

confidence: 97%

“…Serum DAO activity and LPS level are two major makers for intestinal permeability (Yin et al 2015a); in the present study, we found that Trichinella spiralis infection in PI-IBS group significantly lowered serum DAO activity and enhanced LPS concentration compared with the control and HSP70 groups (P < 0.05). Meanwhile, preinduction of HSP70 significantly reversed the effects of Trichinella spiralis infection on intestinal permeability evidenced by the increased DAO activity and reduced LPS levels (P < 0.05) (Fig.…”

Section: Intestinal Permeability and Tight Junction Expressionmentioning

confidence: 91%

Preinduction of heat shock protein 70 protects mice against post-infection irritable bowel syndrome via NF-κB and NOS/NO signaling pathways

et al. 2015

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This study aimed to investigate the protective effects of preinduction of heat shock protein 70 (HSP70) on Trichinella spiralis infection-induced post-infectious irritable bowel syndrome (PI-IBS) in mice. Trichinella spiralis infection significantly reduced HSP70 abundance, ileal villus height and crypt depth, expression of tight junctions, serum lysine and arginine concentrations, and ileal SCL7A6 and SCL7A7 mRNA levels, induced inflammatory response, and activated NF-κB signaling pathway. Meanwhile, the heat treatment upregulated HSP70 expression, and then reversed intestinal dysfunction and inflammatory response. Preinduction of HSP70 enhanced serum arginine and intestinal SCL7A7 expression and inhibited NF-κB activation compared with PI-IBS model. Treatment with pyrrolidine dithiocarbamate (PDTC, an NF-κB inhibitor) and N-nitro-L-arginine methyl ester hydrochloride (L-NAME, a nitric oxide synthase inhibitor, NOS) further demonstrated that preinduction of HSP70 might inhibit NF-κB and activated NOS/nitric oxide (NO) signaling pathways. In conclusion, preinduction of HSP70 by heat treatment may confer beneficial effects on Trichinella spiralis infection-induced PI-IBS in mice, and the protective effect of HSP70 may be associated with inhibition of NF-κB and stimulation of NOS/NO signaling pathways.

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“…reported that TP pretreatment could suppress the TLR4/NF-κB signaling pathway, protecting renal tubular epithelial cells against ischemia/reperfusion-induced apoptosis and inflammation. The mechanism may be that TP blocked phosphorylation and subsequent degradation of inhibitor of κB-α and κB-β subunits (Singh et al, 2011), which contributes to NF-κB activation (Yin et al, 2015b).…”

Section: Discussionmentioning

confidence: 99%

Long-term effects of oral tea polyphenols and Lactobacillus brevis M8 on biochemical parameters, digestive enzymes, and cytokines expression in broilers

Wei

et al. 2015

J. Zhejiang Univ. Sci. B

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This study investigates the long-term effects of oral tea polyphenols (TPs) and Lactobacillus brevis M8 (LB) on biochemical parameters, digestive enzymes, and cytokines expression in broilers. In experiment 1, 240 broiler chickens were selected to investigate the effects of 0.06 g/kg body weight (BW) TP and 1.0 ml/kg BW LB on broilers; in experiment 2, 180 broiler chickens were assigned randomly to three groups to investigate the effects of different dosages of TP (0.03, 0.06, and 0.09 g/kg BW) combined with 1.0 ml/kg BW LB on broilers; in experiment 3, 180 broiler chickens were assigned randomly to three groups to investigate the effects of different dosages of LB (0.5, 1.0, and 1.5 ml/kg BW) combined with 0.06 g/kg BW TP on broilers. The results showed that TP and LB affected serum biochemical parameters, and TP reduced serum cholesterol (CHO) and low-density lipoprotein cholesterol (LDL-C) abundances in a dosage-dependent manner (P<0.05) on Day 84. Meanwhile, broilers fed a diet supplemented with TP or LB had a lower intestinal lipase activity on Day 84 compared with the control group (P<0.05). Middle and high dosages of TP increased pancreatic lipase and proventriculus pepsin activities (P<0.05). Also middle and high dosages of LB significantly enhanced pancreatic lipase activity (P<0.05), while high LB supplementation inhibited intestinal trypsase (P<0.05) on Day 84. Furthermore, both TP and LB reduced intestinal cytokine expression and nuclear factor-κ B (NF-κB) mRNA level on Days 56 and 84. In conclusion, long-term treatment of TP and LB improved lipid metabolism and digestive enzymes activities, and affected intestinal inflammatory status, which may be associated with the NF-κB signal.

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Hydrogen peroxide-induced oxidative stress activates NF-κB and Nrf2/Keap1 signals and triggers autophagy in piglets

Cited by 113 publications

References 35 publications

Transient effects of weaning on the health of newly weaning piglets

Transient effects of weaning on the health of newly weaning piglets

Preinduction of heat shock protein 70 protects mice against post-infection irritable bowel syndrome via NF-κB and NOS/NO signaling pathways

Long-term effects of oral tea polyphenols and Lactobacillus brevis M8 on biochemical parameters, digestive enzymes, and cytokines expression in broilers

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