Preinduction of heat shock protein 70 protects mice against post-infection irritable bowel syndrome via NF-κB and NOS/NO signaling pathways

Abstract

This study aimed to investigate the protective effects of preinduction of heat shock protein 70 (HSP70) on Trichinella spiralis infection-induced post-infectious irritable bowel syndrome (PI-IBS) in mice. Trichinella spiralis infection significantly reduced HSP70 abundance, ileal villus height and crypt depth, expression of tight junctions, serum lysine and arginine concentrations, and ileal SCL7A6 and SCL7A7 mRNA levels, induced inflammatory response, and activated NF-κB signaling pathway. Meanwhile, the heat treatment upregulated HSP70 expression, and then reversed intestinal dysfunction and inflammatory response. Preinduction of HSP70 enhanced serum arginine and intestinal SCL7A7 expression and inhibited NF-κB activation compared with PI-IBS model. Treatment with pyrrolidine dithiocarbamate (PDTC, an NF-κB inhibitor) and N-nitro-L-arginine methyl ester hydrochloride (L-NAME, a nitric oxide synthase inhibitor, NOS) further demonstrated that preinduction of HSP70 might inhibit NF-κB and activated NOS/nitric oxide (NO) signaling pathways. In conclusion, preinduction of HSP70 by heat treatment may confer beneficial effects on Trichinella spiralis infection-induced PI-IBS in mice, and the protective effect of HSP70 may be associated with inhibition of NF-κB and stimulation of NOS/NO signaling pathways.

Heat shock protein 70 protects mouse against post-infection irritable bowel syndrome via up-regulating intestinal γδ T cell’s Th17 response

“…Our previous research reported that the intestinal HSP70 level raised during PI-IBS. Furthermore, Preinduction of HSP70 could improve the clinical symptoms of PI-IBS [ 22 , 26 ]. Several studies also demonstrated that HSP70 inhibits the production of proinflammatory cytokines in different cell populations [ 27 ].…”

“…Expression of HSP70 was induced by heat treatment according to previous report [ 22 ]. The mice were anesthetized with sodium pentobarbital (50 mg/kg).…”

The relevance of functional amino acids to support the health of growing pigs

“…In mice, a Trichinella spiralis infection reduced serum arginine (Arg) concentration and the intestinal expression of the SLC7A7 gene (Zhou et al, 2015) that encodes for the basolateral y + L system transporter with an affinity for lysine (Lys), Arg, glutamine (Gln), histidine (His), methionine (Met) and leucine (Leu). This could be related to an increased use of Arg by the enterocytes.…”

Analgesic, anti-inflammatory and immuno-modulatory effects of dezocine-propofol anesthesia combination following colonoscopy

“…HSP70 is a member of the heat shock protein family which regulates the stability of intracellular peptides [14]. Its secretion is increased in response to inflammatory reactions [14]; IL-1β is an important pro-inflammatory mediator derived from mononuclear macrophages and lymphocytes.…”

“…Its secretion is increased in response to inflammatory reactions [14]; IL-1β is an important pro-inflammatory mediator derived from mononuclear macrophages and lymphocytes. It mediates the amplification cascade of inflammatory reactions [15,16].…”