Hydrogen peroxide enhances phagocytosis of<i>Pseudomonas aeruginosa</i>in hyperoxia

Phan, Binh D; Entezari, Maria; Lockshin, Richard A.; Bartelt, Diana C.; Mantell, Lin L.

doi:10.3109/1547691x.2010.531063

Cited by 3 publications

(2 citation statements)

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“…It has been shown that during hyperoxic stress uptake of retinal outer segments by human RPE increases. 46 Likewise, murine macrophage cells show enhanced phagocytic activity during hyperoxidative stress that was marginalized with VIT-D. 47 It could be concluded that VIT-D might act as a phagocytosis modulator.…”

Section: Discussionmentioning

confidence: 99%

Vitamin-D3 (α-1, 25(OH) 2D3) Protects Retinal Pigment Epithelium From Hyperoxic Insults

Murugeswari

Firoz

Subramani

et al. 2020

Invest. Ophthalmol. Vis. Sci.

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Citation: Murugeswari P, Firoz A, Murali S, et al. Vitamin-D3 (α-1, 25(OH) 2D3) protects retinal pigment epithelium from hyperoxic insults. Invest Ophthalmol Vis Sci. 2020;61(2):4. https://doi.org/10.1167/iovs.61.2.4 PURPOSE.Oxidative stress affects the retinal pigment epithelium (RPE) leading to development of vascular eye diseases. Cholecalciferol (VIT-D) is a known modulator of oxidative stress and angiogenesis. This in vitro study was carried out to evaluate the protective role of VIT-D on RPE cells incubated under hyperoxic conditions. METHODS.Cadaver primary RPE (PRPE) cells were cultured in hyperoxia (40% O 2 ) with or without VIT-D (α-1, 25(OH) 2D3). The functional and physiological effects of PRPE cells with VIT-D treatment were analyzed using molecular and biochemical tools. RESULTS.Vascular signaling modulators, such as vascular endothelial growth factor (VEGF) and Notch, were reduced in hyperoxic conditions but significantly upregulated in the presence of VIT-D. Additionally, PRPE conditioned medium with VIT-D induced the tubulogenesis in primary human umbilical vein endothelial cells (HUVEC) cells. VIT-D supplementation restored phagocytosis and transmembrane potential in PRPE cells cultured under hyperoxia. CONCLUSIONS. VIT-D protects RPE cells and promotes angiogenesis under hyperoxic insult.These findings may give impetus to the potential of VIT-D as a therapeutic agent in hyperoxia induced retinal vascular diseases.Keywords: retinal pigment epithelium, vitamin D, hyperoxia, vascular endothelial growth factor (VEGF), tubulogenesis O xidative stress is the result of an imbalance between the synthesis of reactive oxygen species (ROS) and the levels of antioxidants in the cells. It plays a major role in the pathophysiology of various ocular diseases, such as agerelated cataract, macular degeneration, glaucoma, diabetic retinopathy, and retinitis pigmentosa, by affecting cellular and vascular physiological aspects. 1,2 Antioxidants, such as enzymatic antioxidants, vitamins, minerals, carotenoids, and flavonoids, are the primary scavengers of ROS reducing levels of oxidative stress. 3 Cholecalciferol (vitamin-D3 (VIT-D)) has diverse functions, including modulation of inflammation, angiogenesis, oxidative stress, and fibrosis. 4,5 Recent studies have demonstrated an association between VIT-D and retinal pathophysiological conditions, such as age-related macular degeneration (AMD), diabetic retinopathy, and retinopathy of prematurity (ROP). 6−8 These studies implicated deficiency of VIT-D to higher risk for early/late AMD, whereas a supplementation leads to delay or prevention in the progression of AMD. 6,9 Although VIT-D receptors as well as enzymes for VIT-D metabolism are present in the retina, choroid, and retinal pigment epithelium (RPE) cells, their functions are still not well understood. 6 VIT-D has been studied as a potential inhibitor of angiogenesis in a mouse model of oxygen-induced ischemic retinopathy (OIR). 10 A correlation of VIT-D and vascular endothelial growth factor (VEGF) has been ob...

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Section: Discussionmentioning

confidence: 99%

Vitamin-D3 (α-1, 25(OH) 2D3) Protects Retinal Pigment Epithelium From Hyperoxic Insults

Murugeswari

Firoz

Subramani

et al. 2020

Invest. Ophthalmol. Vis. Sci.

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“…HP exhibits a differential effect on human macrophage phagocytosis, with increased activity at low concentrations and suppressed activity at high concentrations (17). Different bacteria exhibit different levels of susceptibility towards HP.…”

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confidence: 99%

In vitro antibacterial effect of carbamide peroxide on oral biofilm

Yao

Waterfield

Shen

et al. 2013

Journal of Oral Microbiology

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This study compared the effects of carbamide peroxide (CP) and chlorhexidine (CHX) on oral biofilm in vitro. Collagen-coated hydroxyapatite discs were inoculated with subgingival plaque. After 3 weeks, the emergent biofilms were subjected to 1-, 3-, and 10-min exposures of a 1% CHX gel, a 5% CP gel and rinse, and a 10% CP gel and rinse. Subsequently, the biofilms were stained using a two-colour fluorescent dye kit for confocal laser scanning microscopy, and the volume ratio of dead bacteria to all bacteria was analysed. Compared to a non-treated gel control, the active agents killed bacteria on all the discs, with higher concentration and longer exposure times killing more bacteria. The rinse form disrupted the biofilm quicker than the gel form. Overall, 10% CP showed more disruption of biofilm and a greater proportion of killed bacteria than 1% CHX (p<0.05).

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Production of Extracellular Enzymes by Lentinula edodes Strains in Solid-State Fermentation on Lignocellulosic Biomass Sterilized by Physical and Chemical Methods

Silva,

Milagres

2023

Curr Microbiol

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Hydrogen peroxide enhances phagocytosis ofPseudomonas aeruginosain hyperoxia

Cited by 3 publications

References 43 publications

Vitamin-D3 (α-1, 25(OH) 2D3) Protects Retinal Pigment Epithelium From Hyperoxic Insults

Vitamin-D3 (α-1, 25(OH) 2D3) Protects Retinal Pigment Epithelium From Hyperoxic Insults

In vitro antibacterial effect of carbamide peroxide on oral biofilm

Production of Extracellular Enzymes by Lentinula edodes Strains in Solid-State Fermentation on Lignocellulosic Biomass Sterilized by Physical and Chemical Methods

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