Hydrogen Peroxide Biosensors Based on Horseradish Peroxidase and Hemoglobin

Ahammad, A. J. Saleh

doi:10.4172/2155-6210.s9-001

Cited by 36 publications

(38 citation statements)

References 143 publications

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“…Therefore, the second enzyme of HRP and the mediator of catechol were used to further react with hydrogen peroxide. 43 These mediators have the common characteristics of small molecular weight, lower redox potential and reversibility. With this approach, a significant redox peak around À0.2 to À0.3 V was obtained such that these two enzymatic reaction products could be simultaneously detected using potential state.…”

Section: B Enzyme Concentration Evaluationmentioning

confidence: 99%

Multiple enzyme-doped thread-based microfluidic system for blood urea nitrogen and glucose detection in human whole blood

Yang

Lin

2015

Biomicrofluidics

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This research presents a multiple enzyme-doped thread-based microfluidic system for blood urea nitrogen (BUN) and glucose detection in human whole blood. A novel enzyme-doped thread coated with a thin polyvinylchloride (PVC) membrane is produced for on-site electrochemical detection of urea and glucose in whole blood. Multiple enzymes can be directly applied to the thread without delicate pretreatment or a surface modification process prior to sealing the thread with PVC membrane. Results indicate that the developed device exhibits a good linear dynamic range for detecting urea and glucose in concentrations from 0.1 mM-10.0 mM (R(2 )= 0.9850) and 0.1 mM-13.0 mM (R(2 )= 0.9668), which is suitable for adoption in detecting the concentrations of blood urea nitrogen (BUN, 1.78-7.12 mM) and glucose (3.89-6.11 mM) in serum. The detection result also shows that the developed thread-based microfluidic system can successfully separate and detect the ions, BUN, and glucose in blood. The calculated concentrations of BUN and glucose ante cibum (glucose before meal) in the whole blood sample are 3.98 mM and 4.94 mM, respectively. The developed thread-based microfluidic system provides a simple yet high performance for clinical diagnostics.

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Section: B Enzyme Concentration Evaluationmentioning

confidence: 99%

Multiple enzyme-doped thread-based microfluidic system for blood urea nitrogen and glucose detection in human whole blood

Yang

Lin

2015

Biomicrofluidics

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“…Reactions that occurred on the electrode surface are as follows. 39 H2O2 + HRPred  H2O + HRPox (1) HRPox + HQ  HRPred + BQ…”

Section: Chronoamperometric Detection Of Psamentioning

confidence: 99%

Development of Titania Nanotube-based Electrochemical Immunosensor and Determination of Prostate Specific Antigen

et al. 2018

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Early diagnosis of cancer is the most important factor that increases the success of treatment. Therefore, the development of new diagnostic tools is a necessity. In this study, a new electrode surface was developed via modification of a disposable titanium electrode with anodic oxidation and coating of gold nanoparticle and chitosan. Titanium electrodes were anodized by several anodization parameters to obtain a nanoporous surface and characterized by scanning electron microscopy. Electrodes anodized in optimum conditions were modified with gold nanoparticles and chitosan for enhancing conductivity and functionalizing the surface of electrode, respectively. To detect prostate specific antigen (PSA), anti-PSA was bound onto the functional electrode surface. Modified electrodes were characterized with scanning electron microscopy and cyclic voltammetry and used for chronoamperometric detection of PSA. Limit of detection (LOD) of the designed electrode was found to be 7.8 ng mL for PSA in a linear range of 0 - 100 ng mL.

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“…All of this is performed without the need for any additional preanalytical steps. To achieve sufficient shuttling of electrons between the substrate and redox center of the enzyme [33], an electron mediator could be used. We selected thionine for this purpose.…”

Section: Introductionmentioning

confidence: 99%

Magnetic beads-based electrochemical immunosensor for monitoring allergenic food proteins

Čadková

Metelka

Holubová

et al. 2015

Analytical Biochemistry

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Hydrogen Peroxide Biosensors Based on Horseradish Peroxidase and Hemoglobin

Cited by 36 publications

References 143 publications

Multiple enzyme-doped thread-based microfluidic system for blood urea nitrogen and glucose detection in human whole blood

Multiple enzyme-doped thread-based microfluidic system for blood urea nitrogen and glucose detection in human whole blood

Development of Titania Nanotube-based Electrochemical Immunosensor and Determination of Prostate Specific Antigen

Magnetic beads-based electrochemical immunosensor for monitoring allergenic food proteins

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