Hydrogen metabolism in the extreme thermophile Thermotoga neapolitana

d’Ippolito, Giuliana; Dipasquale, Laura; Vella, Filomena Monica; Romano, Ida; Gambacorta, Agata; Cutignano, Adele; Fontana, Angelo

doi:10.1016/j.ijhydene.2009.12.044

Cited by 67 publications

(55 citation statements)

References 19 publications

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“…[6] To study the metabolic features of this process, a stream of 13 CO 2 was bubbled into cultures of the bacterium grown in Tn medium prepared according to our previous studies. [8] Labeled CO 2 was generated through the reaction of 13 C-labeled barium carbonate (Ba 13 CO 3 ) with 1 m HCl under a flow of N 2 as a carrier gas. As reported in Figure 2, the NMR spectra of the fermentation broth of these The heterotrophic bacterium Thermotoga neapolitana produces hydrogen by fermentation of sugars.…”

Section: Resultsmentioning

confidence: 99%

“…Glucose metabolism in T. neapolitana (Scheme 1 a) occurs by EM, [8] which splits the sugar molecule down the middle, converting carbon positions 1, 2, and 3 (and 6, 5 and 4) into the methyl, carbonyl, and carboxyl carbon atoms of pyruvate. Because C-3 (and C-4) of glucose is lost as CO 2 in the conversion of pyruvate to Ac-CoA, standard fermentation of [2-13 C]glucose results in the synthesis of [1][2][3][4][5][6][7][8][9][10][11][12][13] C]acetic acid together with minor levels of [2-13 C]lactic acid.…”

Section: Resultsmentioning

confidence: 99%

“…Because C-3 (and C-4) of glucose is lost as CO 2 in the conversion of pyruvate to Ac-CoA, standard fermentation of [2-13 C]glucose results in the synthesis of [1][2][3][4][5][6][7][8][9][10][11][12][13] C]acetic acid together with minor levels of [2-13 C]lactic acid. [8] The addition of 14 mm NaH 13 CO 3 to these cultures shifted the process toward the production of lactic acid and [1,[2][3][4][5][6][7][8][9][10][11][12][13] C 2 ]lactic acid became the prevailing product of fermentation. The synthesis of this latter compound was recognizable by NMR spectroscopy because vicinal carbon atoms at d = 69.3 (C-2) and 183.3 ppm (C-1) of doubly labeled product resonated as doublets (J = 54.6 Hz) because of 13 C- 13 C homocoupling ( Figure SM1 in the Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

“…Standard culture conditions Unless otherwise specified, anaerobic cultures of T. neapolitana (DSM 4359 T ) were grown at 80 8C in Tn medium [8] supplemented with 28 mm (0.5 % wt/v) glucose and 0.4 % (wt/v) yeast extract/ tryptone by using 120 mL sealed flasks with working volume of 25 mL and pH adjustment to 7.5 with 1 m NaOH. Fermentation batches were inoculated (6 % v/v) at room temperature with precultures (25 mL) grown overnight at 80 8C without shaking in the same medium.…”

Section: Methodsmentioning

confidence: 99%

“…Fermentation products (acetic acid, lactic acid, and alanine) were assessed by 1 H NMR spectroscopy in the presence of 3.8 mm trimethylamine hydrochloride (TMA) as an internal standard. [6,8] After centrifugation at 25 800g, culture medium (0.6 mL) was diluted with 15 % D 2 O/CD 3 OD (2:1 v/v) and transferred to an NMR tube. Spectra were recorded on a Bruker Avance 600 spectrometer operating at 600 MHz for 1 H and equipped with a Cryoprobe.…”

Section: Chemical Analysismentioning

confidence: 99%

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Recycling of Carbon Dioxide and Acetate as Lactic Acid by the Hydrogen‐Producing Bacterium Thermotoga neapolitana

2014

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The heterotrophic bacterium Thermotoga neapolitana produces hydrogen by fermentation of sugars. Under capnophilic (carbon dioxide requiring) conditions, the process is preferentially associated with the production of lactic acid, which, as shown herein, is synthesized by reductive carboxylation of acetyl coenzyme A. The enzymatic coupling is dependent on the carbon dioxide stimulated activity of heterotetrameric pyruvate:ferredoxin oxidoreductase. Under the same culture conditions, T. neapolitana also operates the unfavorable synthesis of lactic acid from an exogenous acetate supply. This process, which requires carbon dioxide (or carbonate) and an unknown electron donor, allows for the conversion of carbon dioxide into added-value chemicals without biomass deconstruction.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Chemical Analysismentioning

confidence: 99%

See 3 more Smart Citations

Recycling of Carbon Dioxide and Acetate as Lactic Acid by the Hydrogen‐Producing Bacterium Thermotoga neapolitana

2014

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Hydrogen Production from Wastewater by Biochemical Methods

Ramprakash

Muthukumar

2019

Encyclopedia of Water

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The environmental impacts associated with fossil fuels and depletion of their resources motivated the development of alternate fuels. Hydrogen is an attractive alternate fuel with high heating value and no environmental impact. Although hydrogen is majorly produced by steam reforming, biochemical production especially from carbohydrate rich wastes is of great interest. Hydrogen production from these wastes is carried out by indirect/direct biophotolysis, dark fermentation, two‐stage fermentation and photofermentation. The major constraints of these processes are low hydrogen evolution rate and less yield at large scale. However, effective pretreatment of substrates and inoculum maximize the yield of hydrogen. The factors influence the hydrogen production include nature of microorganism, biochemical process/reactor, temperature, pH, ionic strength, hydraulic retention time, hydrogen and carbon dioxide partial pressure, organic acid concentration, and C/N ratio. The commercial exploitation of biohydrogen production is hindered by lack of high potential microorganism and bioreactor. Hence, it demands multidisciplinary research to understand the fundamental underlying principles besides the development of microbial strains for industrial applications.

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Biohydrogen production from glucose, molasses and cheese whey by suspended and attached cells of four hyperthermophilic Thermotoga strains

Cappelletti

Bucchi

Mendes

et al. 2012

J of Chemical Tech & Biotech

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BACKGROUND: Bio‐hydrogen production from organic residues is an attractive process that combines energy generation with waste treatment. This work describes hydrogen production from molasses and cheese whey by biofilm and suspended‐cells of four hyperthermophilic Thermotoga spp. For the best performing strain, a preliminary process optimization was performed.RESULTS: In experiments with glucose‐fed T. neapolitana, HEPES was the best‐performing buffer, whereas the highest H2 rate was obtained with the biofilm support exposing the highest specific surface. All the Thermotoga strains tested (T. neapolitana, T. maritima, T. naphtophila, T. petrophila) were able to produce H2 from glucose, molasses and cheese whey under both suspended‐ and attached‐cell conditions. T. neapolitana was the best H2 producer with all three substrates. The best H2 production rate was higher on molasses (1.7 mmolH2 L$_{\rm {medium}}^{-1}$ h−1) than on cheese whey (0.94 mmolH2 L$_{\rm {medium}}^{-1}$ h−1), whereas the highest H2/substrate yields were similar for the two substrates (2.95 molH2 mol$_{\rm {monosaccharide consumed}}^{-1}$ on molasses and 2.50 molH2 mol$_{\rm {monosaccharide consumed}}^{-1}$ on cheese whey). With both wastes, the progressive growth medium simplification allowed a drastic reduction of medium cost, without significant losses of process performance.CONCLUSION: Both molasses and cheese whey are suitable substrates for H2 production by suspended‐ and attached‐cells of Thermotoga species. Copyright © 2012 Society of Chemical Industry

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Hydrogen metabolism in the extreme thermophile Thermotoga neapolitana

Cited by 67 publications

References 19 publications

Recycling of Carbon Dioxide and Acetate as Lactic Acid by the Hydrogen‐Producing Bacterium Thermotoga neapolitana

Recycling of Carbon Dioxide and Acetate as Lactic Acid by the Hydrogen‐Producing Bacterium Thermotoga neapolitana

Hydrogen Production from Wastewater by Biochemical Methods

Biohydrogen production from glucose, molasses and cheese whey by suspended and attached cells of four hyperthermophilic Thermotoga strains

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