Recycling of Carbon Dioxide and Acetate as Lactic Acid by the Hydrogen‐Producing Bacterium <i>Thermotoga neapolitana</i>

d’Ippolito, Giuliana; Dipasquale, Laura; Fontana, Angelo

doi:10.1002/cssc.201402155

Cited by 27 publications

(19 citation statements)

References 18 publications

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“…Thermophilic bacteria, such as Thermotogales, have been acquiring an emerging attention in biotechnology for hydrogen production by digestion of organic residues (Conners et al, 2006;Elleuche et al, 2014;Pradhan et al, 2015). Among thermophilic bacteria, T. neapolitana shows an interesting potential for the simultaneous production of hydrogen and LA by CLF d'Ippolito et al, 2014;Pradhan et al, 2017). In the present study, a selected strain of this bacterium, namely T. neapolitana subsp.…”

Section: Discussionmentioning

confidence: 94%

“…Enhancement of cell reductants, e.g., NAD(P)H or ferredoxin (Fd), represents a major requirement to sustain the simultaneous production of H 2 and LA during CLF d'Ippolito et al, 2014). RNA-seq approach revealed up-regulation of several genes involved in redox reactions and electron transport.…”

Section: Redox Re-organization Under Clf Conditionsmentioning

confidence: 99%

“…In the last years, we reported that T. neapolitana also operates a novel, anaplerotic process named capnophilic lactic fermentation (CLF) for the synthesis of almost enantiopure L-lactic acid (LA) without affecting H 2 production d'Ippolito et al, 2014;Pradhan et al, 2016bPradhan et al, , 2019Nuzzo et al, 2019). The metabolic process is activated by CO 2 (capnophilic means "requiring CO 2 ") and, nominally, is dependent on a Janus pathway including a catabolic branch leading to acetyl-CoA (AcCoA) from sugars by glycolysis, and an anabolic branch that combines AcCoA and CO 2 to give LA through reduction of newly synthesized pyruvate (PYR) by a NADH-dependent lactic dehydrogenase (LDH) (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

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CO2-Induced Transcriptional Reorganization: Molecular Basis of Capnophillic Lactic Fermentation in Thermotoga neapolitana

et al. 2020

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Capnophilic lactic fermentation (CLF) is a novel anaplerotic pathway able to convert sugars to lactic acid (LA) and hydrogen using CO 2 as carbon enhancer in the hyperthermophilic bacterium Thermotoga neapolitana. In order to give further insights into CLF metabolic networks, we investigated the transcriptional modification induced by CO 2 using a RNA-seq approach. Transcriptomic analysis revealed 1601 differentially expressed genes (DEGs) in an enriched CO 2 atmosphere over a total of 1938 genes of the T. neapolitana genome. Transcription of PFOR and LDH genes belonging to the CLF pathway was up-regulated by CO 2 together with 6-phosphogluconolactonase (6PGL) and 6-phosphogluconate dehydratase (EDD) of the Entner-Doudoroff (ED) pathway. The transcriptomic study also revealed up-regulation of genes coding for the flavin-based enzymes NADH-dependent reduced ferredoxin:NADP oxidoreductase (NFN) and NADferredoxin oxidoreductase (RNF) that control supply of reduced ferredoxin and NADH and allow energy conservation-based sodium translocation through the cell membrane. These results support the hypothesis that CO 2 induces rearrangement of the central carbon metabolism together with activation of mechanisms that increase availability of the reducing equivalents that are necessary to sustain CLF. In this view, this study reports a first rationale of the molecular basis of CLF in T. neapolitana and provides a list of target genes for the biotechnological implementation of this process.

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Section: Discussionmentioning

confidence: 94%

Section: Redox Re-organization Under Clf Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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CO2-Induced Transcriptional Reorganization: Molecular Basis of Capnophillic Lactic Fermentation in Thermotoga neapolitana

et al. 2020

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“…Recently, some studies reported that the use of an atmosphere enriched in carbon dioxide stimulated the synthesis of lactate by T. neapolitana, which has similar characteristics to T. maritima. This process was named capnophilic lactic fermentation (fermentative CO 2 -dependent) where lactate formation was the result of the association of exogenous CO 2 with acetyl-CoA [9,47,48]. Dipasquale et al [47] showed that the high production of lactate (up to 0.6 mol mol À1 glucose) Fig.…”

Section: Effect Of Increasing the Amount Of Ahfw On H 2 Productionmentioning

confidence: 99%

Enhancement of fermentative hydrogen production by Thermotoga maritima through hyperthermophilic anaerobic co-digestion of fruit-vegetable and fish wastes

Saidi

Liebgott

Hamdi

et al. 2018

International Journal of Hydrogen Energy

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In this work, different proportions of model fruit and vegetable wastes (MFVW) and acid hydrolyzed fish wastes (AHFW) were used for hydrogen production in a minimum culture medium based on seawater. Experiments were performed in pH-controlled Stirred Tank Reactor (STR) with or without the addition of nitrogen and sulfur sources. The total H 2 production and the maximum hydrogen productivity of T. maritima in the culture medium, containing MFVW and AHFW (45 mmol L À1 carbohydrates) at a C/N ratio of 12, were 132 mmol L À1 and 15 mmol h À1 L À1 , respectively. However, tripling the concentration of carbohydrates to reach a C/N ratio of 22, has increased two times the maximum H 2 productivity (28 mmol h À1 L À1) due to the improvement in nutrient balance. The cumulative H 2 production was 285 mmol L À1 , yielding a potential energy generation of 0.1210 3 MJ ton À1 wastes, which could be an interesting alternative for energy recovery.

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“…The hydrogen production of T. maritima can be boosted via metabolic engineering, as was demonstrated by an in silico redesign of its metabolism (Nogales et al 2012). Additionally, while the degradation of sugars by many Thermotogae results in the production of CO 2 and acetate, T. neapolitana has been shown to convert these byproducts to lactic acid when grown in a CO 2 atmosphere, a process suggested to have potential in carbon capture (D'Ippolito et al 2014).…”

Section: The Thermotogaementioning

confidence: 99%

Insights into thermoadaptation and the evolution of mesophily from the bacterial phylum Thermotogae

2015

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Thermophiles are extremophiles that grow optimally at temperatures >45°C. To survive and maintain function of their biological molecules, they have a suite of characteristics not found in organisms that grow at moderate temperature (mesophiles). At the cellular level, thermophiles have mechanisms for maintaining their membranes, nucleic acids, and other cellular structures. At the protein level, each of their proteins remains stable and retains activity at temperatures that would denature their mesophilic homologs. Conversely, cellular structures and proteins from thermophiles may not function optimally at moderate temperatures. These differences between thermophiles and mesophiles presumably present a barrier for evolutionary transitioning between the 2 lifestyles. Therefore, studying closely related thermophiles and mesophiles can help us determine how such lifestyle transitions may happen. The bacterial phylum Thermotogae contains hyperthermophiles, thermophiles, mesophiles, and organisms with temperature ranges wide enough to span both thermophilic and mesophilic temperatures. Genomic, proteomic, and physiological differences noted between other bacterial thermophiles and mesophiles are evident within the Thermotogae. We argue that the Thermotogae is an ideal group of organisms for understanding of the response to fluctuating temperature and of long-term evolutionary adaptation to a different growth temperature range.Key words: lateral gene transfer, Kosmotoga, Mesotoga, thermostability, stress response. Résumé :Les thermophiles sont des extrémophiles dont la température optimale de croissance se situe au-delà de 45°C. Pour survivre et permettre à leurs biomolécules de bien fonctionner, ils doivent se doter de caractéristiques qu'on ne retrouve pas chez des organismes se développant à des températures intermédiaires (mésophiles). Sur le plan cellulaire, les thermophiles ont des mécanismes permettant de préserver leurs membranes, acides nucléiques et autres structures cellulaires. Au chapitre des protéines, chacune d'entre elles demeure stable et active à des températures qui dénatureraient leurs homologues mésophiles. En revanche, les structures et protéines cellulaires des thermophiles pourraient ne pas fonctionner de manière optimale à des températures intermédiaires. De telles différences entre les thermophiles et les mésophiles auraient tendance à se dresser en travers d'une transition évolutive entre ces deux modes de vie. Par conséquent, l'étude de thermophiles et de mésophiles fortement apparentés pourrait nous permettre d'établir comment surviendraient de telles transitions du mode de vie. Le phylum bactérien Thermotogae regroupe des hyperthermophiles, des thermophiles, des mésophiles et des organismes dont l'intervalle de températures chevauche les plages thermophiles et mésophiles. On observe parmi les Thermotogae des différences sur le plan génomique, protéomique, et physiologique qu'on relève également entre d'autres thermophiles et méso-philes bactériens. Nous soutenons que les Thermot...

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Recycling of Carbon Dioxide and Acetate as Lactic Acid by the Hydrogen‐Producing Bacterium Thermotoga neapolitana

Cited by 27 publications

References 18 publications

CO2-Induced Transcriptional Reorganization: Molecular Basis of Capnophillic Lactic Fermentation in Thermotoga neapolitana

CO2-Induced Transcriptional Reorganization: Molecular Basis of Capnophillic Lactic Fermentation in Thermotoga neapolitana

Enhancement of fermentative hydrogen production by Thermotoga maritima through hyperthermophilic anaerobic co-digestion of fruit-vegetable and fish wastes

Insights into thermoadaptation and the evolution of mesophily from the bacterial phylum Thermotogae

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