Hydrogen Ion and Carbon Dioxide Content of the Oviductal Fluid of the Rhesus Monkey (Macaca Mulatta)

Maas, D.; Storey, Bayard T.; Mastroianni, Luigi

doi:10.1016/s0015-0282(16)42801-3

Cited by 88 publications

(8 citation statements)

References 24 publications

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“…Both the pH and the HCO − 3 concentration of the oviductal fluid are higher in uterine and tubal fluids compared to plasma (Vishwakarma, 1962). Moreover, pH in the rhesus monkey female tract elevates dramatically, concomitantly with ovulation (Maas et al, 1977), which might promote sperm capacitation in vivo. In mammalian spermatozoa, several HCO − 3 transporters were reported as candidates to mediate HCO − 3 influx across the plasma membrane such as Na + /HCO − 3 cotransporter (NBC) (Demarco et al, 2003), Cl − /HCO − 3 exchangers (Chavez et al, 2012), and CFTR (Hernández-González et al, 2007;Xu et al, 2007), as well as its indirect entrance via CO 2 diffusion with subsequent hydration by intracellular carbonic anhydrases (CA) (Wandernoth et al, 2010;José et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Quantitative Intracellular pH Determinations in Single Live Mammalian Spermatozoa Using the Ratiometric Dye SNARF-5F

Chávez

Darszon

Treviño

et al. 2020

Front. Cell Dev. Biol.

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Intracellular pH (pH i) plays a crucial role in mammalian sperm physiology. However, it is a challenging task to acquire quantitative single sperm pH i images due to their small size and beating flagella. In this study, we established a robust pH i imaging system using the dual-emission ratiometric pH indicator, SNARF-5F. Simultaneous good signal/noise ratio fluorescence signals were obtained exciting with a green high-power LED (532 nm) and acquiring with an EM-CCD camera through an image splitter with two band-pass filters (550-600 nm, channel 1; 630-650 nm, channel 2). After in vivo calibration, we established an imaging system that allows determination of absolute pH i values in spermatozoa, minimizing cell movement artifacts. Using this system, we determined that bicarbonate increases non-capacitated human pH i with slower kinetics than in mouse spermatozoa. This difference suggests that distinct ionic transporters might be involved in the bicarbonate influx into human and mouse spermatozoa. Alternatively, pH i regulation downstream bicarbonate influx into spermatozoa could be different between the two species.

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Section: Introductionmentioning

confidence: 99%

Quantitative Intracellular pH Determinations in Single Live Mammalian Spermatozoa Using the Ratiometric Dye SNARF-5F

Chávez

Darszon

Treviño

et al. 2020

Front. Cell Dev. Biol.

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“…Several authors proposed multiphase fluid models for cartilage that include osmotic effects. There is evidence that certain ionic constituents (e.g., CaCl 2 , NaCl, KCl, pH) of cervical mucus show cyclic variations during the menstrual cycle 11,12. It is however not fully understood how these changes affect mucus hydration and its structure and mechanical (viscoelastic) properties.…”

Section: Introductionmentioning

confidence: 99%

Ionic and pH effects on the osmotic properties and structure of polyelectrolyte gels

Horkay

Basser

2008

J Polym Sci B Polym Phys

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ABSTRACT:We investigate the effects of salt concentration and pH on neutralized poly(acrylic acid) (PAA) gels in near physiological salt solutions. Either adding calcium ions or decreasing the pH is found to induce reversible volume transitions but the nature of these transitions seems to be different. For example, the osmotic pressure exhibits a simple power law dependence on the concentration as the transition is approached in both systems, but the power law exponent n is substantially different in the two cases. On decreasing the pH the value of n gradually increases from 2.1 (at pH ¼ 7) to 3.2 (at pH ¼ 1). By contrast, n decreases with increasing calcium ion concentration from 2.1 (in 100 mM NaCl solution) to 1.6 (0.8 mM CaCl 2 in 100 mM NaCl solution). In both systems, a strong increase of the small-angle neutron scattering intensity (SANS) is observed near the volume transition. The SANS results reveal that calcium ions favor the formation of linearly aligned regions in PAA gels.

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“…The changes underlying the response to bicarbonate could take place in the upper portions of the female tract around the time of ovulation. Female oviductal fluids have a high concentration of bicarbonate (35 mM), which rises after ovulation (to 90 mM) [13], and therefore such fluids may trigger in vivo the responses described here. These changes could occur either when spermatozoa are kept 'repressed' in the lower isthmus (while attached to the oviductal epithelium), or soon after they start their journey towards the oocyte to achieve fertilization.…”

Section: Resultsmentioning

confidence: 91%

“…This was done by investigating whether exposure to bicarbonate, a component of in vitro fertilization media which destabilizes sperm membranes over a short period of time [11], appears to be essential for capacitation [12], and is present in high concentrations in oviductal fluid [13], results in rapid changes in sperm membrane lipids. We have also investigated whether a short exposure to bicarbonate modifies spermatozoa in such a way that they are rendered capable of undergoing exocytosis upon challenge with a natural agonist.…”

Section: Present Addressmentioning

confidence: 99%

Bicarbonate/CO₂ induces rapid activation of phospholipase A₂ and renders boar spermatozoa capable of undergoing acrosomal exocytosis in response to progesterone

Roldán

Vázquez

1996

FEBS Letters

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We tested whether capacitation, a phenomenon that renders spermatozoa capable of undergoing acrosomal exocytosis, may be completed rapidly after a short exposure to bicarbonatelCO2. We found that, in the presence of Ca 2+, a 10-min exposure of boar spermatozoa to bicarbonate led to a partial activation of phospholipase A2, primed spermatozoa for a major subsequent activation of this enzyme upon stimulation with progesterone and furthermore rendered spermatozoa capable of undergoing exocytosis in response to this steroid. These results suggest that capacitation may be completed in a relatively short period of time and open up new possibilities for unravelling molecular mechanisms underlying this process.

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Hydrogen Ion and Carbon Dioxide Content of the Oviductal Fluid of the Rhesus Monkey (Macaca Mulatta)

Cited by 88 publications

References 24 publications

Quantitative Intracellular pH Determinations in Single Live Mammalian Spermatozoa Using the Ratiometric Dye SNARF-5F

Quantitative Intracellular pH Determinations in Single Live Mammalian Spermatozoa Using the Ratiometric Dye SNARF-5F

Ionic and pH effects on the osmotic properties and structure of polyelectrolyte gels

Bicarbonate/CO₂ induces rapid activation of phospholipase A₂ and renders boar spermatozoa capable of undergoing acrosomal exocytosis in response to progesterone

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Hydrogen Ion and Carbon Dioxide Content of the Oviductal Fluid of the Rhesus Monkey (Macaca Mulatta)

Cited by 88 publications

References 24 publications

Quantitative Intracellular pH Determinations in Single Live Mammalian Spermatozoa Using the Ratiometric Dye SNARF-5F

Quantitative Intracellular pH Determinations in Single Live Mammalian Spermatozoa Using the Ratiometric Dye SNARF-5F

Ionic and pH effects on the osmotic properties and structure of polyelectrolyte gels

Bicarbonate/CO2 induces rapid activation of phospholipase A2 and renders boar spermatozoa capable of undergoing acrosomal exocytosis in response to progesterone

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Bicarbonate/CO₂ induces rapid activation of phospholipase A₂ and renders boar spermatozoa capable of undergoing acrosomal exocytosis in response to progesterone