Hydrogen fermentation using lactate as the sole carbon source: Solution for ‘blind spots’ in biofuel production

Ohnishi, Akihiro; Hasegawa, Yuji; Abe, Shinko; Bando, Yukiko; Fujimoto, Naoshi; Suzuki, Masaharu

doi:10.1039/c2ra20590d

Cited by 34 publications

(21 citation statements)

References 39 publications

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“…This result agrees with the results obtained by Ohnishi et al [29], where Megasphaera elsdenii was the dominant genus (30e58% of the microflora), and its hydrogen producing capability was confirmed. Megasphaera sp.…”

Section: Characterization Of the Microbial Communitysupporting

confidence: 95%

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Biohydrogen from food waste in a discontinuous process: Effect of HRT and microbial community analysis

Moreno‐Andrade

Carrillo‐Reyes

Santiago

et al. 2015

International Journal of Hydrogen Energy

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Section: Characterization Of the Microbial Communitysupporting

confidence: 95%

“…It is also important to note that in media with glucose and lactate, lactate was consumed faster [33]. According to the metabolites reported in Table 2, the presence OTU 526 (>85%), presumably related to M. elsdenii, may follow a pathway that consumes lactic acid, which is large hydrogen producer [29].…”

Section: Characterization Of the Microbial Communitymentioning

confidence: 99%

Biohydrogen from food waste in a discontinuous process: Effect of HRT and microbial community analysis

Moreno‐Andrade

Carrillo‐Reyes

Santiago

et al. 2015

International Journal of Hydrogen Energy

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“…Megaspahera was found in hydrogen‐producing reactors treating several residual effluents, including wastewater from the sugarcane industry and organic fraction of solid wastes . Hydrogen production by Megasphaera has been reported to be linked to lactic acid production . Hence, although lactic acid was not measured in this work, lactic acid bacteria related to genera Lactobacillus , Lactococcus , Mitsuokella , Sporolactobacillus , and Bifidobacterium were found at a relative abundance of ∼12% (relative to the total recovered sequences), most of them previously reported in hydrogen‐producing reactors .…”

Section: Resultsmentioning

confidence: 99%

Biohydrogen production from winery effluents: control of the homoacetogenesis through the headspace gas recirculation

Buitrón

Muñoz-Páez

Quijano

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND: Fermentative hydrogen production has an inherent limitation caused by hydrogen-consuming metabolic pathways such as homoacetogenesis related to high hydrogen partial pressures. In this study, a strategy based on recirculating the headspace gas was applied to increase the hydrogen release from the liquid to the gas phase in an upflow anaerobic sludge blanket(UASB) reactor fed with winery effluents. The influence of the gas upflow recirculation velocity on hydrogen production, hydrogen consumption by homoacetogenesis, and microbial community structure was evaluated.RESULTS: Under the control condition (only liquid recirculation), the hydrogen productivity was as much as 22 mL H 2 L −1 h −1 . Conversely, the hydrogen productivity increased up to 62 mL H 2 L −1 h −1 when the reactor was operated with an upflow gas recirculation velocity of 28.6 m d −1 . The increase in mass transfer, due to the gas recirculation, produces a decrease (up to 70%) in the hydrogen consumption rate associated with homoacetogenesis. High-throughput 16s rDNA sequencing characterization showed that the gas recirculation strategy promoted the development of hydrogen-producing microorganisms related to Megasphaera elsdenii and decreased the abundance of hydrogen-consuming bacteria related to Clostridium carboxidivorans and C. ljungdahlii. CONCLUSION:The results indicated that headspace recirculation at gas upflow velocities higher than 17.8 m d −1 increased the hydrogen production rate concomitantly with the reduction of both the homoacetogenic activity and the abundance of H 2 -consuming bacteria. The study demonstrated that headspace recirculation could be a promising way to control homoacetogenesis, and therefore, to increase the biohydrogen productivity from complex substrates such as winery effluents. ABBREVIATIONS CODchemical oxygen demand CSTR continuous stirred tank reactor EtOH residual ethanol (mg COD L -1 ) HA homoacetogenic activity HAc acetic acid (mg COD L -1 ) HBu butyric acid (mg COD L -1 ) HCap caproic acid (mg COD L -1 ) HC-H hydrogen consumers by homoacetogenesis HP-C hydrogen producer by carbohydrates HP-LA hydrogen producers by lactic acid HPR hydrogen production rate HPr propionic acid (mg COD L -1 ) HRT hydraulic retention time HVal valeric acid (mg COD L -1 ) m mixing degree MEE missing electron equivalents OTUs operational taxonomic units t mix mixing time UASB upflow anaerobic sludge blanket WWs winery wastewater effluents Homoacetogenic activityThe acetate production by homoacetogenic activity (HA) was calculated using the stoichiometric relation of hydrogen, acetate, J Chem Technol Biotechnol 2020; 95: 544-552

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“…The oxidation of lactate to acetate also yields hydrogen and carbon dioxide as well as other products such as propionate [ 4 , 15 , 45 ]. Furthermore, anaerobic oxidation of butyrate or propionate (present in the processed medium at low concentrations) requires syntrophic metabolic processes that generate hydrogen, formate, and carbon dioxide used directly by partner hydrogenotrophic methanogens [ 9 ].…”

Section: Discussionmentioning

confidence: 99%

“…Lactate can also act as a substrate for the non-methanogen Archaeoglobus , a known sulfate reducer capable of oxidizing lactate to carbon dioxide [ 1 , 9 ]. It has also been demonstrated that lactate can be used as a sole carbon source and oxidized to acetate, propionate, and hydrogen by Megasphaera elsdenii [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Methane-yielding microbial communities processing lactate-rich substrates: a piece of the anaerobic digestion puzzle

Detman

Mielecki

Pleśniak

et al. 2018

Biotechnol Biofuels

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BackgroundAnaerobic digestion, whose final products are methane and carbon dioxide, ensures energy flow and circulation of matter in ecosystems. This naturally occurring process is used for the production of renewable energy from biomass. Lactate, a common product of acidic fermentation, is a key intermediate in anaerobic digestion of biomass in the environment and biogas plants. Effective utilization of lactate has been observed in many experimental approaches used to study anaerobic digestion. Interestingly, anaerobic lactate oxidation and lactate oxidizers as a physiological group in methane-yielding microbial communities have not received enough attention in the context of the acetogenic step of anaerobic digestion. This study focuses on metabolic transformation of lactate during the acetogenic and methanogenic steps of anaerobic digestion in methane-yielding bioreactors.ResultsMethane-yielding microbial communities instead of pure cultures of acetate producers were used to process artificial lactate-rich media to methane and carbon dioxide in up-flow anaerobic sludge blanket reactors. The media imitated the mixture of acidic products found in anaerobic environments/digesters where lactate fermentation dominates in acidogenesis. Effective utilization of lactate and biogas production was observed. 16S rRNA profiling was used to examine the selected methane-yielding communities. Among Archaea present in the bioreactors, the order Methanosarcinales predominated. The acetoclastic pathway of methane formation was further confirmed by analysis of the stable carbon isotope composition of methane and carbon dioxide. The domain Bacteria was represented by Bacteroidetes, Firmicutes, Proteobacteria, Synergistetes, Actinobacteria, Spirochaetes, Tenericutes, Caldithrix, Verrucomicrobia, Thermotogae, Chloroflexi, Nitrospirae, and Cyanobacteria. Available genome sequences of species and/or genera identified in the microbial communities were searched for genes encoding the lactate-oxidizing metabolic machinery homologous to those of Acetobacterium woodii and Desulfovibrio vulgaris. Furthermore, genes for enzymes of the reductive acetyl-CoA pathway were present in the microbial communities.ConclusionsThe results indicate that lactate is oxidized mainly to acetate during the acetogenic step of AD and this comprises the acetotrophic pathway of methanogenesis. The genes for lactate utilization under anaerobic conditions are widespread in the domain Bacteria. Lactate oxidation to the substrates for methanogens is the most energetically attractive process in comparison to butyrate, propionate, or ethanol oxidation.Electronic supplementary materialThe online version of this article (10.1186/s13068-018-1106-z) contains supplementary material, which is available to authorized users.

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Hydrogen fermentation using lactate as the sole carbon source: Solution for ‘blind spots’ in biofuel production

Cited by 34 publications

References 39 publications

Biohydrogen from food waste in a discontinuous process: Effect of HRT and microbial community analysis

Biohydrogen from food waste in a discontinuous process: Effect of HRT and microbial community analysis

Biohydrogen production from winery effluents: control of the homoacetogenesis through the headspace gas recirculation

Methane-yielding microbial communities processing lactate-rich substrates: a piece of the anaerobic digestion puzzle

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