Hydrogels of agarose, and methacrylated gelatin and hyaluronic acid are more supportive for in vitro meniscus regeneration than three dimensional printed polycaprolactone scaffolds

Bahçecioğlu, Gökhan; Hasırcı, Nesrin; Bilgen, Bahar; Hasırcı, Vasıf

doi:10.1016/j.ijbiomac.2018.09.065

Cited by 59 publications

(66 citation statements)

References 44 publications

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“…2D). Similar observations were reported in our previous study [2]. Gelatin has biologic recognition sites such as arginine-glycine-aspartic acid (RGD) sequences which promote cell adhesion [35].…”

Section: Cell Morphology and Gene Expression On Pcl Scaffolds And Hydsupporting

confidence: 87%

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Anatomical meniscus construct with zone specific biochemical composition and structural organization

Bahçecioğlu

Bilgen

Hasırcı

et al. 2019

Preprint

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A PCL/hydrogel construct that would mimic the structural organization, biochemistry and anatomy of meniscus was engineered. The compressive (380 ± 40 kPa) and tensile modulus (18.2 ± 0.9 MPa) of the PCL scaffolds were increased significantly when constructs were printed with a shifted design and circumferential strands mimicking the collagen organization in native tissue (p<0.05). Gene expression of human fibrochondrocytes in agarose (Ag), gelatin methacrylate (GelMA), and GelMA-Ag hydrogels was significantly higher than that of cells on the PCL scaffolds after a 21-day culture. GelMA exhibited the highest level of collagen type I (COL1A2) mRNA expression, while GelMA-Ag exhibited the highest level of aggrecan (AGG) expression (p<0.001, compared to PCL). GelMA and GelMA-Ag exhibited a high level of collagen type II (COL2A1) expression (p<0.05, compared to PCL). Anatomical scaffolds with circumferential PCL strands were impregnated with cell-loaded GelMA in the periphery and GelMA-Ag in the inner region. GelMA and GelMA-Ag hydrogels enhanced the production of COL 1 and COL 2 proteins after a 6-week culture (p<0.05). COL 1 expression increased gradually towards the outer periphery, while COL 2 expression decreased. We were thus able to engineer an anatomical meniscus with a cartilage-like inner region and fibrocartilage-like outer region.

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Section: Cell Morphology and Gene Expression On Pcl Scaffolds And Hydsupporting

confidence: 87%

“…The COL 2/COL 1 ratio was 10-20% in the outer region and increased to 60-80% towards the inner region. This biochemical composition is very close to that of the native meniscus, at which COL 1 constitutes 80% of the outer region and COL 2 constitutes 60% of the inner region [2].…”

Section: Production Of Structurally Biochemically and Anatomically Csupporting

confidence: 53%

Anatomical meniscus construct with zone specific biochemical composition and structural organization

Bahçecioğlu

Bilgen

Hasırcı

et al. 2019

Preprint

Self Cite

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“…Hydrogels also have important potential and have been shown to promote higher levels of ECM secretion than polycaprolactone alone. GelMA promoted collagen synthesis and agarose/MeHA promoted GAG synthesis, the former important for cell differentiation of the inner meniscus and the latter important for the outer [52]. The key point to take from this is that there are many potential materials that could generate a successful scaffold, as well as a multitude of growth factors and additives that could support tissue growth and delay degradation.…”

Section: Discussionmentioning

confidence: 99%

Are the Biological and Biomechanical Properties of Meniscal Scaffolds Reflected in Clinical Practice? A Systematic Review of the Literature

Ranmuthu

Russell

et al. 2019

IJMS

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The aim of this PRISMA review was to assess whether the CMI and Actifit scaffolds, when used in clinical practice, improve clinical outcomes and demonstrate the ideal biological and biomechanical properties of scaffolds: being chondroprotective, porous, resorbable, able to mature and promote regeneration of tissue. This was done by only including studies that assessed clinical outcome and used a scale to assess both integrity of the scaffold and its effects on articular cartilage via MRI. A search was performed on PubMed, EMBASE, Scopus and clinicaltrials.gov. 2457 articles were screened, from which eight studies were selected: four used Actifit, three used CMI and one compared the two. All studies reported significant improvement in at least one clinical outcome compared to baseline. Some studies suggested that the scaffolds appeared to show porosity, mature, resorb and/or have possible chondroprotective effects, as assessed by MRI. The evidence for clinical translation is limited by differences in study methodology and small sample sizes, but is promising in terms of improving clinical outcomes in the short to mid-term. Higher level evidence, with MRI and histological evaluation of the scaffold and articular cartilage, is now needed to further determine whether these scaffolds exhibit these useful properties.

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“…50-fold after being seeded with porcine fibrochondrocytes. In a related study, porcine fibrochondrocyte-seeded hydrogels, such as agarose, GelMA, HAMA, and GelMA-HAMA were combined with 3D printed PCL scaffolds and evaluated under static and dynamic compression conditions (Bahcecioglu et al, 2019). After 35 days, cell carrying hydrogels produced higher levels of ECM components than the 3D printed PCL control.…”

Section: D Printing Applicationsmentioning

confidence: 99%

3D and 4D Printing of Polymers for Tissue Engineering Applications

Tamay

Usal

Alagoz

et al. 2019

Front. Bioeng. Biotechnol.

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316

239

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Three-dimensional (3D) and Four-dimensional (4D) printing emerged as the next generation of fabrication techniques, spanning across various research areas, such as engineering, chemistry, biology, computer science, and materials science. Three-dimensional printing enables the fabrication of complex forms with high precision, through a layer-by-layer addition of different materials. Use of intelligent materials which change shape or color, produce an electrical current, become bioactive, or perform an intended function in response to an external stimulus, paves the way for the production of dynamic 3D structures, which is now called 4D printing. 3D and 4D printing techniques have great potential in the production of scaffolds to be applied in tissue engineering, especially in constructing patient specific scaffolds. Furthermore, physical and chemical guidance cues can be printed with these methods to improve the extent and rate of targeted tissue regeneration. This review presents a comprehensive survey of 3D and 4D printing methods, and the advantage of their use in tissue regeneration over other scaffold production approaches.

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Hydrogels of agarose, and methacrylated gelatin and hyaluronic acid are more supportive for in vitro meniscus regeneration than three dimensional printed polycaprolactone scaffolds

Cited by 59 publications

References 44 publications

Anatomical meniscus construct with zone specific biochemical composition and structural organization

Anatomical meniscus construct with zone specific biochemical composition and structural organization

Are the Biological and Biomechanical Properties of Meniscal Scaffolds Reflected in Clinical Practice? A Systematic Review of the Literature

3D and 4D Printing of Polymers for Tissue Engineering Applications

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