Hydrogels for Large-Scale Expansion of Stem Cells

Preparation and properties of silk fibroin hydrogel for biological dressing

“…3B. It showed the fluorescence detection results of cells cultured on the gel of different silk fibroin concentrations under Al-B indicator [24]. From the chart, we could see that the fluorescence detection value of three gel materials changed little in the first three days, and the reduction rate of Al-B increased rapidly from third days to seventh days later, 43.85% and 46.25%, respectively, lower than a group (47.03%).…”

Recent advances in 3D hydrogel culture systems for mesenchymal stem cell-based therapy and cell behavior regulation

“…Free radical polymerization induced crosslink, enzymatic induced crosslink, Diels–Alder “click” reaction, Schiff base formation, oxime formation and Michael type-addition are all typically involved chemically crosslinking methods during the formation of hydrogels that are biocompatible for cell encapsulation in recent years. 51,52 Compared to physical crosslinking, chemical crosslinking forms covalent bonds among polymer chains. The linkages are stronger and most of them are irreversible which means they can be the stabilizer of the hydrogel.…”

“…Engineering an appropriate mechanical microenvironment for encapsulating stem cells is beneficial to achieve controlled stem cell differentiation, which is conductive to the therapeutic effect of stem cell-based therapy. 24 Hydrogel can be directly implanted into the action site achieving sustained release, and its applications in tissue repairing and regenerative medicine adopt similar strategies as the stem-cell-based therapy. It is remarkable that the complexity and heterogeneity of native mechanical environment limit the possibility to perfectly provide an in vivo ECM-liked microenvironment for cells.…”

“…20 Cell apoptosis will be caused by the shortage of cell–matrix interactions leading to a suboptimal cell survival rate. 24 These synthetic models have provided more and more insights into how MSC behaviors and related indicator expressions respond to different kinds of stimuli; in this way we can design an in vitro niche for the future use of stem cell-based therapies.…”

Advanced Bio-Based Polymers for Astrocyte Cell Models

“…Hydrogels are hydrophilic polymers commonly used in regenerative medicine and for reconstructive purposes [124]. They form a network that provides a suitable extracellular environment for cellular infiltration and a scaffold for cell ingrowth and matrix deposition.…”