Hydrogel platform facilitating astrocytic differentiation through cell mechanosensing and YAP-mediated transcription

Liu, Zhongqian; Mao, Shijie; Hu, Yunbin; Liu, Feng; Shao, Xiaowei

doi:10.1016/j.mtbio.2023.100735

Cited by 4 publications

(4 citation statements)

References 44 publications

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“…Its regulation mechanism to promote differentiation may involve the hydrogel spontaneously de-crosslinking changing its mechanical properties, transferring mechanical stimulation, activating the Piezo 1 calcium channel, promoting YAP nuclear transcription through the actin cytoskeleton, and eventually stimulating astrocyte differentiation. This provides new insights into the mechanism of mechanical regulation of astrocyte development ( 80 ). Tuina treatment involves regular mechanical stimulation, and activating the Piezo 1 calcium channel may be a key mechanism of its effect.…”

Section: The Association Between Tcm Rehabilitation and Novel Biomate...mentioning

confidence: 99%

Advances in novel biomaterials combined with traditional Chinese medicine rehabilitation technology in treatment of peripheral nerve injury

Liu,

Hu,

Huang

et al. 2024

Front. Neurol.

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Peripheral nerve injuries (PNI) represent one of the primary neuropathies leading to lifelong disability. Nerve regeneration and targeted muscle atrophy stand as the two most crucial factors influencing functional rehabilitation post peripheral nerve injury. Over time, traditional Chinese medicine (TCM) rehabilitation approaches such as acupuncture, Tuina, and microneedles serve as pivot means to activate the regeneration of injured nerve Schwann cells. By promoting axon regeneration, these approaches can accomplish nerve repair, reconstruction, and functional rehabilitation. Although TCM rehabilitation approaches have clinically demonstrated effectiveness in promoting the repair and regeneration of PNI, the related molecular mechanisms remain unclear. This significantly hampers the application and promotion of TCM rehabilitation in PNI recovery. Therefore, deeply delving into the cellular and molecular mechanisms of TCM rehabilitation technologies to foster nerve regeneration stands as the most pressing issue. On the other hand, in recent years, novel biomaterials represented by hydrogels, microfluidic platforms, and new chitosan scaffolds have showed their unique roles in treating various degrees of nerve injury. These methods exhibit immense potential in conducting high-throughput cell and organoid culture in vitro and synthesizing diverse tissue engineering scaffolds and drug carriers. We believe that the combination of TCM rehabilitation technology and novel biomaterials can more effectively address precise treatment issues such as identification of treatment target and dosage control. Therefore, this paper not only summarizes the molecular mechanisms of TCM rehabilitation technology and novel biomaterials in treating peripheral nerve injury individually, but also explores the research direction of precise treatment by integrating the two at both macro and micro levels. Such integration may facilitate the exploration of cellular and molecular mechanisms related to neurodegeneration and regeneration, providing a scientific and theoretical foundation for the precise functional rehabilitation of PNI in the future.

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Section: The Association Between Tcm Rehabilitation and Novel Biomate...mentioning

confidence: 99%

Advances in novel biomaterials combined with traditional Chinese medicine rehabilitation technology in treatment of peripheral nerve injury

Liu,

Hu,

Huang

et al. 2024

Front. Neurol.

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“…Besides, it is worth noting that except responding to mechanical stimuli, YAP is also an important transcription factor. In addition to physical therapy, materials such as hydrogel and drugs such as Dapagliflozin and Icariin have also been shown to affect the progression of diseases through regulating Hippo/YAP signaling pathway [134][135][136][137]. As mentioned above, temperature or mechanical sensitive materials can be used to treat diseases by regulating YAP.…”

Section: Therapeutic Potential and Current Constraintsmentioning

confidence: 99%

The roles of Hippo/YAP signaling pathway in physical therapy

Pan,

Hao,

Deng

et al. 2024

Cell Death Discov.

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Cellular behavior is regulated by mechanical signals within the cellular microenvironment. Additionally, changes of temperature, blood flow, and muscle contraction also affect cellular state and the development of diseases. In clinical practice, physical therapy techniques such as ultrasound, vibration, exercise, cold therapy, and hyperthermia are commonly employed to alleviate pain and treat diseases. However, the molecular mechanism about how these physiotherapy methods stimulate local tissues and control gene expression remains unknow. Fortunately, the discovery of YAP filled this gap, which has been reported has the ability to sense and convert a wide variety of mechanical signals into cell-specific programs for transcription, thereby offering a fresh perspective on the mechanisms by which physiotherapy treat different diseases. This review examines the involvement of Hippo/YAP signaling pathway in various diseases and its role in different physical therapy approaches on diseases. Furthermore, we explore the potential therapeutic implications of the Hippo/YAP signaling pathway and address the limitations and controversies surrounding its application in physiotherapy.

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“…7 Meanwhile, some studies have shown that culture under the conditions of the hydrogel can significantly shorten the directed differentiation time of cells. 8 In particular, 3D bioprinting technology based on hydrogels can achieve precise regulation of the internal microstructure of materials, which is conducive not only to the spatial distribution of cells but also to the matching of the materials with organisms. 9 Studies have confirmed that 3D bioprinted hepatorganoids can realize the functions of liver albumin secretion, drug metabolism, and glycogen storage and even prolong the survival time of mice with liver failure after transplantation in vivo.…”

Section: ■ Introductionmentioning

confidence: 99%

“…More importantly, regulating cells by changing the external physical conditions can reduce the overuse of molecular compounds and thus avoid inducing potentially unknown differentiation states in cells . Meanwhile, some studies have shown that culture under the conditions of the hydrogel can significantly shorten the directed differentiation time of cells . In particular, 3D bioprinting technology based on hydrogels can achieve precise regulation of the internal microstructure of materials, which is conducive not only to the spatial distribution of cells but also to the matching of the materials with organisms .…”

Section: Introductionmentioning

confidence: 99%

Biomimetic Hydrogels Promote Pseudoislet Formation to Improve Glycemic Control in Diabetic Mice

Zhu,

Xu,

et al. 2024

ACS Biomater. Sci. Eng.

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Islet or β-cell transplantation is currently considered to be the ideal treatment for diabetes, and three-dimensional (3D) bioprinting of a bionic pancreas with physiological stiffness is considered to be promising for the encapsulation and transplantation of β-cells. In this study, a 5%GelMA/2%AlgMA hybrid hydrogel with pancreatic physiological stiffness was constructed and used for β-cell encapsulation, 3D bioprinting, and in vivo transplantation to evaluate glycemic control in diabetic mice. The hybrid hydrogel had good cytocompatibility and could induce insulin-producing cells (IPCs) to form pseudoislet structures and improve insulin secretion. Furthermore, we validated the importance of betacellulin (BTC) in IPCs differentiation and confirmed that IPCs self-regulation was achieved by altering the nuclear and cytoplasmic distributions of BTC expression. In vivo transplantation of diabetic mice quickly restored blood glucose levels. In the future, 3D bioprinting of β-cells using biomimetic hydrogels will provide a promising platform for clinical islet transplantation for the treatment of diabetes.

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Hydrogel platform facilitating astrocytic differentiation through cell mechanosensing and YAP-mediated transcription

Cited by 4 publications

References 44 publications

Advances in novel biomaterials combined with traditional Chinese medicine rehabilitation technology in treatment of peripheral nerve injury

Advances in novel biomaterials combined with traditional Chinese medicine rehabilitation technology in treatment of peripheral nerve injury

The roles of Hippo/YAP signaling pathway in physical therapy

Biomimetic Hydrogels Promote Pseudoislet Formation to Improve Glycemic Control in Diabetic Mice

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