Hydrogel microspheres for stabilization of an antioxidant enzyme: Effect of emulsion cross-linking of a dual polysaccharide system on the protection of enzyme activity

Tang, Deh Wei; Yu, Shu Huei; Wu, Wen‐Hsin; Hsieh, Hao Ying; Tsai, Yi Chin; Mi, Fwu Long

doi:10.1016/j.colsurfb.2013.09.002

Cited by 24 publications

(16 citation statements)

References 37 publications

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“…TED-loaded chitosan microspheres were prepared by the emulsion cross-linking method. 22 , 23 In short, TED (20 mg) and chitosan (115 mg) were added to 5 mL of dichloromethane. After complete dissolution, the solution was slowly added to the solution of 1% Span 80, and then the mixed solution was emulsified with a propeller agitator at 50× g for 15 minutes.…”

Section: Methodsmentioning

confidence: 99%

A novel tetrandrine-loaded chitosan microsphere: characterization and in vivo evaluation

Guo

Cang

2016

DDDT

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In this study, novel tetrandrine-loaded chitosan microspheres were prepared by the emulsion cross-linking method. The systems were then characterized for physicochemical properties and in vitro drug release. In addition, the pharmacokinetics and tissue distribution of microspheres were further verified in animal models. Particle-size distribution indicated that the size of microspheres was within the range of 7–15 μm, with a median diameter of 12.4 μm. The drug loading and entrapment efficiency of the formulation were 34.6%±12.5% and 87.3%±9.7% (mean ± SD), respectively. In vitro release showed a typical sustained and long-term drug release behavior. The Higuchi equation was the model that fit best with release data. Maintaining a relatively constant plasma concentration in the long-term drug treatment is an outstanding pharmacokinetic advantage of tetrandrine microspheres in vivo. Moreover, compared with tetrandrine solution, tetrandrine microspheres produced a lower drug concentration in the heart, liver, and kidneys. This indicated that the microspheres used in this study were preferable for targeting lung tissue versus other tissues. No damage to the tissues of the lung was found in histopathological examination.

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Section: Methodsmentioning

confidence: 99%

A novel tetrandrine-loaded chitosan microsphere: characterization and in vivo evaluation

Guo

Cang

2016

DDDT

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“…Often, the emulsion protocols include harsh nonaqueous solvents that can lead to cytotoxicity issues or poor process control, resulting in highly variable microsphere shapes and sizes. [14][15][16] Patterned molds are a way to circumvent the cytotoxicity issues and still create microspheres. 17,18 However, scaling these methods to the levels needed for transplants can be challenging.…”

Section: Introductionmentioning

confidence: 99%

A Versatile Microencapsulation Platform for Hyaluronic Acid and Polyethylene Glycol

Harrington

Ott²,

Karanu³

et al. 2021

Tissue Engineering Part A

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Cell microencapsulation is a rapidly expanding field with broad potential for stem cell therapies and tissue engineering research. Traditional alginate microspheres suffer from poor biocompatibility, and microencapsulation of more advanced hydrogels is challenging due to their slower gelation rates. We have developed a novel, noncytotoxic, nonemulsion-based method to produce hydrogel microspheres compatible with a wide variety of materials, called core-shell spherification (CSS). Fabrication of microspheres by CSS derived from two slow-hardening hydrogels, hyaluronic acid (HA) and polyethylene glycol diacrylate (PEGDA), was characterized. HA microspheres were manufactured with two different crosslinking methods: thiolation and methacrylation. Microspheres of methacrylated HA (MeHA) had the greatest swelling ratio, the largest average diameter, and the lowest diffusion barrier. In contrast, PEGDA microspheres had the smallest diameters, the lowest swelling ratio, and the highest diffusion barrier, while microspheres of thiolated HA had characteristics that were in between the other two groups. To test the ability of the hydrogels to protect cells, while promoting function, diabetic NOD mice received intraperitoneal injections of PEGDA or MeHA microencapsulated canine islets. PEGDA microspheres reversed diabetes for the length of the study (up to 16 weeks). In contrast, islets encapsulated in MeHA microspheres at the same dose restored normoglycemia, but only transiently (3-4 weeks). Nonencapsulated canine islet transplanted at the same dose did not restore normoglycemia for any length of time. In conclusion, CSS provides a nontoxic microencapsulation procedure compatible with various hydrogel types.

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“…Enzyme entrapment in hydrogels is one of the important approaches that retains the activity and conformation of the enzyme in a physiological environment (Kojuharova et al, 1988;Shiroya et al, 1995;Kim et al, 2011). Biomaterials such as chitosan and hyaluronic acid (Tang et al, 2014;Sun et al, 2015), or chemical materials such as poly(ethyleneglycol) (PEG) (Bayramoglu and Arica, 2014), are often used to provide a hydrogel base. The capacity of these materials for immobilized enzymes is increased by crosslinking, co-polymerization, or functionalization (Bayramoglu and Arica, 2014;Tang et al, 2014;Sun et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Biomaterials such as chitosan and hyaluronic acid (Tang et al, 2014;Sun et al, 2015), or chemical materials such as poly(ethyleneglycol) (PEG) (Bayramoglu and Arica, 2014), are often used to provide a hydrogel base. The capacity of these materials for immobilized enzymes is increased by crosslinking, co-polymerization, or functionalization (Bayramoglu and Arica, 2014;Tang et al, 2014;Sun et al, 2015). However, an immobilized enzyme in a hydrogel is prone to leaking out of the gel because the enzyme is typically simply encapsulated within the small pores of the gel.…”

Section: Introductionmentioning

confidence: 99%

Streptavidin-hydrogel prepared by sortase A-assisted click chemistry for enzyme immobilization on an electrode

Matsumoto

Isogawa

Tanaka

et al. 2018

Biosensors and Bioelectronics

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A tetrameric streptavidin (SA)-appended LPETG tag was site-specifically linked to azido-containing tri-glycine via sortase A catalysis and the resulting azido-modified SA (SA-N3) was retained in the biotin-binding pocket. SA-N3 was polymerized with dibenzylcyclooctyne-modified branched poly(ethyleneglycol) (DBCO-PEG) using azido-modified branched PEG (N3-PEG) as a spacer via copper-free click chemistry. The resulting SA-based hydrogel exhibited gel-like mechanical properties and could immobilize biotin-modified molecules through biotin-SA affinity. Glucose dehydrogenase (GDH) was immobilized in the SA-based hydrogel, and the hydrogel was then coated on a glassy carbon electrode (GCE) and used for the biocatalytic oxidation of glucose. The designed GCE exhibited better performance and stability compared with GDH chemically adsorbed onto a GCE. In addition, the designed GCE anode and a Pt-carbon cathode were assembled into a glucose/O fuel cell that provided a maximum power density and open circuit voltage of 11.8±0.56µWcm and 0.17V, respectively.

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Hydrogel microspheres for stabilization of an antioxidant enzyme: Effect of emulsion cross-linking of a dual polysaccharide system on the protection of enzyme activity

Cited by 24 publications

References 37 publications

A novel tetrandrine-loaded chitosan microsphere: characterization and in vivo evaluation

A novel tetrandrine-loaded chitosan microsphere: characterization and in vivo evaluation

A Versatile Microencapsulation Platform for Hyaluronic Acid and Polyethylene Glycol

Streptavidin-hydrogel prepared by sortase A-assisted click chemistry for enzyme immobilization on an electrode

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