Hydrogel matrix for three enzyme entrapment in creatine/creatinine amperometric biosensing

Schneider, Jörg J.; Gründig, Bernd; Renneberg, Reinhard; Cammann, Karl; Madaras, Marcel B.; Buck, Richard P.; Vorlop, Klaus‐Dieter

doi:10.1016/0003-2670(96)00031-1

Cited by 66 publications

(26 citation statements)

References 13 publications

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“…Therefore, ammonium ions produced during enzymatic urea hydrolysis change the potential of the electrode. This changing is proportional to the urea concentration [25][26][27].…”

Section: Introductionmentioning

confidence: 97%

New Urea Biosensor Based on Urease Enzyme Obtained from Helycobacter pylori

Dindar

Karakuş

Abasiyanik

2011

Appl Biochem Biotechnol

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The urease enzyme of Helicobacter pylori was isolated from biopsy sample obtained from antrum big curvature cell extracts. A new urea biosensor was prepared by immobilizing urease enzyme isolated from Helicobacter pylori on poly(vinylchloride) (PVC) ammonium membrane electrode by using nonactine as an ammonium ionophore. The effect of pH, buffer concentration, and temperature for the biosensor prepared with urease from H. pylori were obtained as 6.0, 5 mM, and 25 °C, respectively. We also investigated urease concentration, stirring rate, and enzyme immobilization procedures in response to urea of the enzyme electrode. The linear working range of the biosensor extends from 1 × 10(-5) to 1 × 10(-2) M and they showed an apparent Nernstian response within this range. Urea enzyme electrodes prepared with urease enzymes obtained from H. pylori and Jack bean based on PVC membrane ammonium-selective electrode showed very good analytical parameters: high sensitivity, dynamic stability over 2 months with less decrease of sensitivity, response time 1-2 min. The analytical characteristics were investigated and were compared those of the urea biosensor prepared with urease enzyme isolated from Jack bean prepared at the same conditions. It was observed that rapid determinations of human serum urea amounts were also made possible with both biosensors.

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“…Therefore, ammonium ions produced during enzymatic urea hydrolysis change the potential of the electrode. This changing is proportional to the urea concentration [25][26][27].…”

Section: Introductionmentioning

confidence: 97%

New Urea Biosensor Based on Urease Enzyme Obtained from Helycobacter pylori

Dindar

Karakuş

Abasiyanik

2011

Appl Biochem Biotechnol

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“…To counteract interference from endogenous creatine at the second step of the catalytic cycle, researchers have used the dual-sensor approach, which measures and subtracts the response for creatine from creatinine. [28][29][30][31][32][33] Amperometry usually requires a system consisting of a reference, a counter (auxiliary), and working electrodes. The most common reference electrodes are the Ag/AgCl or the saturated Hg 2 Cl 2 ; the counter electrode is usually an inert metal such as platinum or stainless steel.…”

Section: Amperometric Creatinine Biosensorsmentioning

confidence: 99%

“…18,21,28,29,31,[33][34][35][36][37][38] It is obvious that platinum has provided a highly catalytic surface where H 2 O 2 oxidation can proceed at an accelerated rate. Although composite materials are a cheaper alternative, they do not reach the operational standards that precious metal surfaces are capable of.…”

Section: Amperometric Creatinine Biosensorsmentioning

confidence: 99%

Electrochemical Creatinine Biosensors

2008

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To measure creatinine, electrochemical techniques have been coupled with a range of biological recognition elements in a variety of sensor configurations. (To listen to a podcast about this feature, please go to the Analytical Chemistry website at pubs.acs.org/ac.The measurement of creatinine levels in human blood or urine is clinically essential because the levels partially reflect the state of renal and muscle function. Creatinine is naturally produced by the body and is filtered from the bloodstream by the kidneys in relatively constant amounts every day. The normal physiological concentration is 40-150 µM, but it can exceed 1000 µM in certain pathological conditions. Blood levels >150 µM indicate the need to perform tests such as creatinine clearance. Values >500 µM indicate severe renal impairment, ultimately leading to dialysis or transplantation; 1 levels <40 µM indicate decreased muscle mass.The methods most often used for the clinical determination of creatinine are based on colorimetry. 2 However, the methods are affected by numerous metabolites and drugs found in biological samples, such as glucose, fructose, ketone bodies, ascorbic acid, and cephalosporins. 3,4 Introducing enzymes has increased specificity, but the methods also became complicated and less reliable. Large and expensive benchtop analyzers incorporating a number of electrochemical electrodes have been used in central clinical laboratories. Portable and handheld devices incorporating a single-use creatinine biosensor cartridge also have been used.The goal of biosensor engineering in the clinical laboratory setting is to reduce cost, time, and complexity of routine analysis of biological fluids; to enable near-patient testing of blood, urine, and saliva in medical centers; and ultimately to enable home testing by individuals. 5 This article looks at the developments in electrochemical creatinine biosensor research in terms of sensor design and analytical performance on the basis of the recognition element and the nature of transducer. Parameters and specific performance characteristics to consider include cost (<$10/ sensing strip), response time (<1.5 minutes [min]), detection limit (e10 µM), linear range (10-1000 µM), and lifetime (>1 year).

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“…Schneider et al (Schneider et al 1996) and Erlenkotter et al(Erlenkotter et al 2002) reported a poly(carbamoyl)sulfonate (PCS) hydrogel biosensor that presented significant improvement in storage stability (ca. 3 months at 8 C) and dynamic range.…”

Section: Introductionmentioning

confidence: 98%

Creatinine and urea biosensors based on a novel ammonium ion-selective copper-polyaniline nano-composite

Zhybak

Beni

Vagin

et al. 2016

Biosensors and Bioelectronics

101

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The use of a novel ammonium ion-specific copper-polyaniline nano-composite as transducer for hydrolase-based biosensors is proposed. In this work, a combination of creatinine deaminase and urease has been chosen as a model system to demonstrate the construction of urea and creatinine biosensors to illustrate the principle. Immobilisation of enzymes was shown to be a crucial step in the development of the biosensors; the use of glycerol and lactitol as stabilisers resulted in a significant improvement, especially in the case of the creatinine, of the operational stability of the biosensors (from few hours to at least 3 days). The developed biosensors exhibited high selectivity towards creatinine and urea. The sensitivity was found to be 85 ± 3.4 mAM(-1)cm(-2) for the creatinine biosensor and 112 ± 3.36 mAM(-1)cm(-2) for the urea biosensor, with apparent Michaelis-Menten constants (KM,app), obtained from the creatinine and urea calibration curves, of 0.163 mM for creatinine deaminase and 0.139 mM for urease, respectively. The biosensors responded linearly over the concentration range 1-125 µM, with a limit of detection of 0.5 µM and a response time of 15s. The performance of the biosensors in a real sample matrix, serum, was evaluated and a good correlation with standard spectrophotometric clinical laboratory techniques was found.

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Hydrogel matrix for three enzyme entrapment in creatine/creatinine amperometric biosensing

Cited by 66 publications

References 13 publications

New Urea Biosensor Based on Urease Enzyme Obtained from Helycobacter pylori

New Urea Biosensor Based on Urease Enzyme Obtained from Helycobacter pylori

Electrochemical Creatinine Biosensors

Creatinine and urea biosensors based on a novel ammonium ion-selective copper-polyaniline nano-composite

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