Hydrocarbon-oxidizing potential and the genes for n-alkane biodegradation in a new acidophilic mycobacterial association from sulfur blocks

Иванова, А. Е.; Сухачева, М. В.; Kanat’eva, A. Yu.; Кравченко, И. К.; Kurganov, A. A.

doi:10.1134/s0026261714060095

Cited by 12 publications

(9 citation statements)

References 17 publications

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“…In our previous work (Ivanova et al, 2014), we identified acidophilic mycobacteria AG S10 exhibiting high catabolic capacity toward hydrocarbons of different chemical structure (paraffins, lowmolecular weight arenes, and aromatic polycyclic hydrocarbons) at extremely low pH values. During 25 days, their cultures growing at 30°C and pH 2.5 performed complete degradation of different alkanes with medium-length straight chain, such as n-undecane, n-dodecane, n-tridecane, and n-tetradecane.…”

Section: Resultsmentioning

confidence: 99%

“…The study was performed with acidophilic mycobacteria AG S10 isolated from a specimen of elementary block sulfur (Ivanova et al, 2014). The cultures were grown under static conditions in hermetically sealed flasks with a liquid mineral medium (Ivanova et al, 2013) containing hydrocarbons as the sole carbon and energy source (0.1-0.2% vol/vol) and the gaseous phase of air (volume ratio, 1 : 10) at 30°C and pH 2.5.…”

Section: Methodsmentioning

confidence: 99%

“…Several enrichment cultures of aerobic bacteria catabolizing hydrocarbons of different structures at pH values below 3.0 were previously isolated from an open sulfur storage site located on the grounds of the Astrakhan gas-processing plant. It was found that these acidophilic bacterial associations were dominated by slowly growing mycobacteria whose genomes contain the genes encoding two hydroxylase families, alkB and Cyp153, which oxidize n-alkanes with medium-length chain, C 14 -C 17 (Ivanova et al, 2013(Ivanova et al, , 2014.…”

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Hydrocarbon biodegradation and surfactant production by acidophilic mycobacteria

2016

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Production of biosurfactants by acidophilic mycobacteria was demonstrated in the course of aerobic degradation of hydrocarbons (n-tridecane, n-tricosane, n-hexacosane, model mixtures of С 14 -С 17 , С 12 -С 19 , and С 9 -С 21 n-alkanes, 2,2,4,4,6,8,8-heptamethylnonane, squalane, and butylcyclohexane) and their complex mixtures (hydrocarbon gas condensate, kerosene, black oil, and paraffin oil) under extremely acidic conditions (pH 2.5). When grown on hydrocarbons, the studied bacterial culture AG S10 caused a decrease in the surface and interfacial tension of the solutions (to the lowest observed values of 26.0 and 1.3 mN/m, respectively) compared to the bacteria-free control. The rheological characteristics of the culture changed only when mycobacteria were grown on hydrocarbons. Neither the medium nor the cell-free culture liquid had the surfactant activity, which indicated formation of an endotype biosurfactant by mycobacteria. Biodegradation of n-alkanes was accompanied by an increase in cell numbers, surfactant production, and changes in the hydrophobicity of bacterial cell surface and in associated phenomena of adsorption and desorption to the hydrocarbon phase. Research on AG S10 culture liquids containing the raw biosurfactant demonstrated the preservation of its activity within a broad range of pH, temperature, and salinity.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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Hydrocarbon biodegradation and surfactant production by acidophilic mycobacteria

2016

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“…Mycobacterium is also a predominant bacterial genus found in extreme acidic sulfur block environments and alkane degradation of strain AG S10 , an acidophilic Mycobacterium , was demonstrated. Interestingly strain AG S10 has both alkB and cyp153 genes, implying the possibility of two alkane hydroxylase families in many acidophilic Mycobacteria strains ( Ivanova et al, 2014 ).…”

Section: Phylogenetic Affiliation and Distribution Of Alkane-utilizinmentioning

confidence: 99%

“…Most alkane-degrading extremophiles have ubiquitous aerobic or anaerobic alkane oxidation pathways represented by ω-oxidation or fumarate addition, respectively ( Figure 1 ). It is assumed that most aerobic psychrophiles, halophiles, acidophiles, and thermophiles degrade alkanes through general alkane oxidation pathways, as demonstrated by the possession of AlkB, CYP, AlmA, and LadA-type alkane hydroxylases ( Bertrand et al, 2013 ; Nie et al, 2013 ; Ivanova et al, 2014 ). However, some extremophiles oxidize alkanes via a unique pathway and produce energy via alternative pathways to avoid stress under harsh conditions.…”

Section: Energy Production Of Extremophiles During Alkane Metabolismmentioning

confidence: 99%

Survival and Energy Producing Strategies of Alkane Degraders Under Extreme Conditions and Their Biotechnological Potential

Park

2018

Front. Microbiol.

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Many petroleum-polluted areas are considered as extreme environments because of co-occurrence of low and high temperatures, high salt, and acidic and anaerobic conditions. Alkanes, which are major constituents of crude oils, can be degraded under extreme conditions, both aerobically and anaerobically by bacteria and archaea of different phyla. Alkane degraders possess exclusive metabolic pathways and survival strategies, which involve the use of protein and RNA chaperones, compatible solutes, biosurfactants, and exopolysaccharide production for self-protection during harsh environmental conditions such as oxidative and osmotic stress, and ionic nutrient-shortage. Recent findings suggest that the thermophilic sulfate-reducing archaeon Archaeoglobus fulgidus uses a novel alkylsuccinate synthase for long-chain alkane degradation, and the thermophilic Candidatus Syntrophoarchaeum butanivorans anaerobically oxidizes butane via alkyl-coenzyme M formation. In addition, gene expression data suggest that extremophiles produce energy via the glyoxylate shunt and the Pta-AckA pathway when grown on a diverse range of alkanes under stress conditions. Alkane degraders possess biotechnological potential for bioremediation because of their unusual characteristics. This review will provide genomic and molecular insights on alkane degraders under extreme conditions.

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Bio-prospective of Acidophile Microorganisms in Biodegradation of NSAIDs

Ratnasari,

Zainiyah,

Thakur

et al. 2024

Curr Pollution Rep

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Hydrocarbon-oxidizing potential and the genes for n-alkane biodegradation in a new acidophilic mycobacterial association from sulfur blocks

Cited by 12 publications

References 17 publications

Hydrocarbon biodegradation and surfactant production by acidophilic mycobacteria

Hydrocarbon biodegradation and surfactant production by acidophilic mycobacteria

Survival and Energy Producing Strategies of Alkane Degraders Under Extreme Conditions and Their Biotechnological Potential

Bio-prospective of Acidophile Microorganisms in Biodegradation of NSAIDs

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