Hydrocarbon degradation by a newly isolated thermophilic Anoxybacillus sp. with bioemulsifier production and new alkB genes

Xia, Wenjie; Dong, Hao; Chang, Zheng; Cui, Qiu; He, Panqing; Tang, Yongchun

doi:10.1039/c5ra17137g

Cited by 15 publications

(6 citation statements)

References 54 publications

(76 reference statements)

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“…Several species of Geobacillus have recently been described as degraders of different hydrocarbons and may use a wide variety of hydrocarbons, especially crude oil, kerosene, and phenanthrene ( Marchant and Banat, 2010 ). Strains of Brevibacillus and Anoxybacillus have also been identified as hydrocarbon degraders under thermophilic conditions ( Xia et al, 2015 ), such as Brevibacillus sp. PDM-3, which degraded phenanthrene as a carbon source ( Reddy et al, 2010 ).…”

Section: Discussionmentioning

confidence: 99%

“…One of the studies reported that Anoxybacillus sp. WJ-4 produced an oligosaccharide–lipid–peptide bioemulsifier with a high emulsification index of 60% ( Xia et al, 2015 ). A strain of Brevibacillus brevis isolated from an oil reservoir produced a lipopeptide-classified biosurfactant with an 80% emulsification index ( Mouafi et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

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Polyphasic Analysis Reveals Potential Petroleum Hydrocarbon Degradation and Biosurfactant Production by Rare Biosphere Thermophilic Bacteria From Deception Island, an Active Antarctic Volcano

et al. 2022

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Extreme temperature gradients in polar volcanoes are capable of selecting different types of extremophiles. Deception Island is a marine stratovolcano located in maritime Antarctica. The volcano has pronounced temperature gradients over very short distances, from as high as 100°C in the fumaroles to subzero next to the glaciers. These characteristics make Deception a promising source of a variety of bioproducts for use in different biotechnological areas. In this study, we isolated thermophilic bacteria from sediments in fumaroles at two geothermal sites on Deception Island with temperatures between 50 and 100°C, to evaluate the potential capacity of these bacteria to degrade petroleum hydrocarbons and produce biosurfactants under thermophilic conditions. We isolated 126 thermophilic bacterial strains and identified them molecularly as members of genera Geobacillus, Anoxybacillus, and Brevibacillus (all in phylum Firmicutes). Seventy-six strains grew in a culture medium supplemented with crude oil as the only carbon source, and 30 of them showed particularly good results for oil degradation. Of 50 strains tested for biosurfactant production, 13 showed good results, with an emulsification index of 50% or higher of a petroleum hydrocarbon source (crude oil and diesel), emulsification stability at 100°C, and positive results in drop-collapse, oil spreading, and hemolytic activity tests. Four of these isolates showed great capability of degrade crude oil: FB2_38 (Geobacillus), FB3_54 (Geobacillus), FB4_88 (Anoxybacillus), and WB1_122 (Geobacillus). Genomic analysis of the oil-degrading and biosurfactant-producer strain FB4_88 identified it as Anoxybacillus flavithermus, with a high genetic and functional diversity potential for biotechnological applications. These initial culturomic and genomic data suggest that thermophilic bacteria from this Antarctic volcano have potential applications in the petroleum industry, for bioremediation in extreme environments and for microbial enhanced oil recovery (MEOR) in reservoirs. In addition, recovery of small-subunit rRNA from metagenomes of Deception Island showed that Firmicutes is not among the dominant phyla, indicating that these low-abundance microorganisms may be important for hydrocarbon degradation and biosurfactant production in the Deception Island volcanic sediments.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Polyphasic Analysis Reveals Potential Petroleum Hydrocarbon Degradation and Biosurfactant Production by Rare Biosphere Thermophilic Bacteria From Deception Island, an Active Antarctic Volcano

et al. 2022

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“…and Anoxybacillus sp. such as Geobacillus caldoxylosilyticus (Marchant et al, 2002), Geobacillus kaustophilus TERI NSM (Sood and Lal, 2008), Geobacillus stearothermophilus KTCC-B7S (Sorkhoh et al, 1993), and Anoxybacillus rupiensis (Xia et al, 2015) were found to utilize various alkane hydrocarbons. Some geobacilli has been transferred to Parageobacillus genus (e.g., Parageobacillus caldoxylosilyticus) (Aliyu et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Microbial Biodegradation of Paraffin Wax in Malaysian Crude Oil Mediated by Degradative Enzymes

et al. 2020

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The deposition of paraffin wax in crude oil is a problem faced by the oil and gas industry during extraction, transportation, and refining of crude oil. Most of the commercialized chemical additives to prevent wax are expensive and toxic. As an environmentally friendly alternative, this study aims to find a novel thermophilic bacterial strain capable of degrading paraffin wax in crude oil to control wax deposition. To achieve this, the biodegradation of crude oil paraffin wax by 11 bacteria isolated from seawater and oil-contaminated soil samples was investigated at 70 • C. The bacteria were identified as Geobacillus kaustophilus N3A7, NFA23, DFY1, Geobacillus jurassicus MK7, Geobacillus thermocatenulatus T7, Parageobacillus caldoxylosilyticus DFY3 and AZ72, Anoxybacillus geothermalis D9, Geobacillus stearothermophilus SA36, AD11, and AD24. The GCMS analysis showed that strains N3A7, MK7, DFY1, AD11, and AD24 achieved more than 70% biodegradation efficiency of crude oil in a short period (3 days). Notably, most of the strains could completely degrade C 37-C 40 and increase the ratio of C 14-C 18 , especially during the initial 2 days incubation. In addition, the degradation of crude oil also resulted in changes in the pH of the medium. The degradation of crude oil is associated with the production of degradative enzymes such as alkane monooxygenase, alcohol dehydrogenase, lipase, and esterase. Among the 11 strains, the highest activities of alkane monooxygenase were recorded in strain AD24. A comparatively higher overall alcohol dehydrogenase, lipase, and esterase activities were observed in strains N3A7, MK7, DFY1, AD11, and AD24. Thus, there is a potential to use these strains in oil reservoirs, crude oil processing, and recovery to control wax deposition. Their ability to withstand high temperature and produce degradative enzymes for long-chain hydrocarbon degradation led to an increase in the short-chain hydrocarbon ratio, and subsequently, improving the quality of the oil.

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“…So far, there is only one reported study on genus Anoxybacillus on alkane hydroxylase involved in crude oil degradation as recorded by Anoxybacillus sp. WJ-4 [8]. Another study demonstrated that organic solvent tolerant Anoxybacillus sp.…”

Section: Growth and Enzyme Analysis Of Isolatesmentioning

confidence: 99%

“…The presence of complex compounds in crude oil such as alkanes, monocyclic, and polycyclic aromatic hydrocarbons causes toxicity to the environment and human health [2,4,5]. Crude oil biodegradation has been widely studied for the past few decades [6][7][8][9]. These studies have led to numerous microorganisms and their enzymes to be isolated and characterized.…”

Section: Introductionmentioning

confidence: 99%

Newly Isolated Alkane Hydroxylase and Lipase Producing Geobacillus and Anoxybacillus Species Involved in Crude Oil Degradation

et al. 2020

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Isolation and studies of novel, crude oil biodegrading thermophilic strains may provide a wider knowledge in understanding their role in petroleum degradation. In this study, the screening of ten new thermophilic strains revealed that all strains were alkane hydroxylase producers and seven of them produced lipase concurrently. Three best strains were characterized and identified through 16S rRNA sequence analysis as Geobacillus sp. D4, Geobacillus sp. D7, and Anoxybacillus geothermalis D9 with GenBank accession numbers MK615934.1, MK615935.1, and MK615936.1, respectively. Gas chromatography (GC) analysis showed that all three strains were able to breakdown various compounds in crude oil such as alkanes, toxic poly-aromatic hydrocarbons (PAHs), organosulfur, carboxylic acids, alkene, resins, organosilicon, alcohol, organochlorine, and ester. For the first time, alkane hydroxylase and lipase activity as well as crude oil degradation by A. geothermalis species were reported. Geobacillus sp. D7 is the best alkane degrader followed by A. geothermalis D9 and Geobacillus sp. D4 with 17.3%, 13.1%, and 12.1% biodegradation efficiency (BE%), respectively. The potential of thermophiles isolated can be explored further for bioremediation of sites polluted by petroleum and oil spills.

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Hydrocarbon degradation by a newly isolated thermophilic Anoxybacillus sp. with bioemulsifier production and new alkB genes

Abstract: In this work, a new thermophilic bacterial strain was isolated and identified as Anoxybacillus sp. WJ-4. This strain of WJ-4 can degrade a wide range of hydrocarbons, and production of an oligosaccharide–lipid–peptide bioemulsifier was detected.

Cited by 15 publications

References 54 publications

Polyphasic Analysis Reveals Potential Petroleum Hydrocarbon Degradation and Biosurfactant Production by Rare Biosphere Thermophilic Bacteria From Deception Island, an Active Antarctic Volcano

Polyphasic Analysis Reveals Potential Petroleum Hydrocarbon Degradation and Biosurfactant Production by Rare Biosphere Thermophilic Bacteria From Deception Island, an Active Antarctic Volcano

Microbial Biodegradation of Paraffin Wax in Malaysian Crude Oil Mediated by Degradative Enzymes

Newly Isolated Alkane Hydroxylase and Lipase Producing Geobacillus and Anoxybacillus Species Involved in Crude Oil Degradation

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