Newly Isolated Alkane Hydroxylase and Lipase Producing Geobacillus and Anoxybacillus Species Involved in Crude Oil Degradation

Yusoff, Durratul Fatini; Rahman, Raja Noor Zaliha Raja Abd; Masomian, Malihe; Ali, Mohd Shukuri Mohamad; Leow, Adam Thean Chor

doi:10.3390/catal10080851

Cited by 21 publications

(10 citation statements)

References 41 publications

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“…However, only a few studies have assessed the potential of members of Anoxybacillus for use in this field. For example, A. geothermalis is able to degrade crude oil while producing alkane hydroxylase and lipase ( Yusoff et al, 2020 ); and Anoxybacillus sp. WJ-4, a new thermophilic strain isolated in the Daqing oilfield, is capable of utilizing alkanes (C8–C22) as a sole source of carbon for growth and produced an oligosaccharide–lipid–peptide bioemulsifier with an EI% over 60%, with increased cell-surface lipophilicity to 65% during hydrocarbon degradation ( Xia et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Polyphasic Analysis Reveals Potential Petroleum Hydrocarbon Degradation and Biosurfactant Production by Rare Biosphere Thermophilic Bacteria From Deception Island, an Active Antarctic Volcano

et al. 2022

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Extreme temperature gradients in polar volcanoes are capable of selecting different types of extremophiles. Deception Island is a marine stratovolcano located in maritime Antarctica. The volcano has pronounced temperature gradients over very short distances, from as high as 100°C in the fumaroles to subzero next to the glaciers. These characteristics make Deception a promising source of a variety of bioproducts for use in different biotechnological areas. In this study, we isolated thermophilic bacteria from sediments in fumaroles at two geothermal sites on Deception Island with temperatures between 50 and 100°C, to evaluate the potential capacity of these bacteria to degrade petroleum hydrocarbons and produce biosurfactants under thermophilic conditions. We isolated 126 thermophilic bacterial strains and identified them molecularly as members of genera Geobacillus, Anoxybacillus, and Brevibacillus (all in phylum Firmicutes). Seventy-six strains grew in a culture medium supplemented with crude oil as the only carbon source, and 30 of them showed particularly good results for oil degradation. Of 50 strains tested for biosurfactant production, 13 showed good results, with an emulsification index of 50% or higher of a petroleum hydrocarbon source (crude oil and diesel), emulsification stability at 100°C, and positive results in drop-collapse, oil spreading, and hemolytic activity tests. Four of these isolates showed great capability of degrade crude oil: FB2_38 (Geobacillus), FB3_54 (Geobacillus), FB4_88 (Anoxybacillus), and WB1_122 (Geobacillus). Genomic analysis of the oil-degrading and biosurfactant-producer strain FB4_88 identified it as Anoxybacillus flavithermus, with a high genetic and functional diversity potential for biotechnological applications. These initial culturomic and genomic data suggest that thermophilic bacteria from this Antarctic volcano have potential applications in the petroleum industry, for bioremediation in extreme environments and for microbial enhanced oil recovery (MEOR) in reservoirs. In addition, recovery of small-subunit rRNA from metagenomes of Deception Island showed that Firmicutes is not among the dominant phyla, indicating that these low-abundance microorganisms may be important for hydrocarbon degradation and biosurfactant production in the Deception Island volcanic sediments.

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Section: Discussionmentioning

confidence: 99%

Polyphasic Analysis Reveals Potential Petroleum Hydrocarbon Degradation and Biosurfactant Production by Rare Biosphere Thermophilic Bacteria From Deception Island, an Active Antarctic Volcano

et al. 2022

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“…The majority of the identified bacteria belonged to the genera Geobacillus and Parageobacillus. Strain D9 was identified previously as Anoxybacillus geothermalis D9 (MK615936.1) (Yusoff et al, 2020). The isolation and identification of these strains at 70 • C suggest a possible role of these microorganisms to degrade crude oil in a high-temperature environment.…”

Section: Discussionmentioning

confidence: 84%

“…The break down of hydrocarbons into simpler molecules allow their assimilation and further degradation within the cell by intracellular enzymes, thus increasing degradation efficiency due to less hydrocarbon being present in the medium (Wasoh et al, 2019). A recent study by Yusoff et al (2020) found that strain D9 exhibited extracellular alkane hydroxylase activity on the first day, but the activity reduced following the increase of the intracellular alkane hydroxylase activity. Further increase in biodegradation efficiency after the enzyme activity declined could also be due to the higher (more than 80%) removal of long-chain n-alkane (C 18 to C 37 ), especially by strains N3A7, MK7, DFY1, AD11, and AD24 on the 3 rd day of incubation.…”

Section: Discussionmentioning

confidence: 95%

Microbial Biodegradation of Paraffin Wax in Malaysian Crude Oil Mediated by Degradative Enzymes

et al. 2020

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The deposition of paraffin wax in crude oil is a problem faced by the oil and gas industry during extraction, transportation, and refining of crude oil. Most of the commercialized chemical additives to prevent wax are expensive and toxic. As an environmentally friendly alternative, this study aims to find a novel thermophilic bacterial strain capable of degrading paraffin wax in crude oil to control wax deposition. To achieve this, the biodegradation of crude oil paraffin wax by 11 bacteria isolated from seawater and oil-contaminated soil samples was investigated at 70 • C. The bacteria were identified as Geobacillus kaustophilus N3A7, NFA23, DFY1, Geobacillus jurassicus MK7, Geobacillus thermocatenulatus T7, Parageobacillus caldoxylosilyticus DFY3 and AZ72, Anoxybacillus geothermalis D9, Geobacillus stearothermophilus SA36, AD11, and AD24. The GCMS analysis showed that strains N3A7, MK7, DFY1, AD11, and AD24 achieved more than 70% biodegradation efficiency of crude oil in a short period (3 days). Notably, most of the strains could completely degrade C 37-C 40 and increase the ratio of C 14-C 18 , especially during the initial 2 days incubation. In addition, the degradation of crude oil also resulted in changes in the pH of the medium. The degradation of crude oil is associated with the production of degradative enzymes such as alkane monooxygenase, alcohol dehydrogenase, lipase, and esterase. Among the 11 strains, the highest activities of alkane monooxygenase were recorded in strain AD24. A comparatively higher overall alcohol dehydrogenase, lipase, and esterase activities were observed in strains N3A7, MK7, DFY1, AD11, and AD24. Thus, there is a potential to use these strains in oil reservoirs, crude oil processing, and recovery to control wax deposition. Their ability to withstand high temperature and produce degradative enzymes for long-chain hydrocarbon degradation led to an increase in the short-chain hydrocarbon ratio, and subsequently, improving the quality of the oil.

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“…This could be due to the presence of hydrocarbon degrading enzymes such as laccases, manganese peroxidases lignin peroxidases and lipase. Yusoff et al (2020) reported that lipase is involved in inducing the lipolytic reactions of emulsified hydrocarbonat the lipid-water interface, thus assisting the hydrocarbon uptake and showed showed the presence of lipase intracellularly or extracellularly produced by hydrocarbon degraders Joutey et al (2014) reported that fungi are effective, especially when it comes to breaking down natural polymeric materials, because they have extracellular multi-enzyme complexes. They can also colonize and penetrate substrates quickly, because of their hyphal networks, they can transfer and redistribute nutrients throughout their mycelium.…”

Section: Discussionmentioning

confidence: 99%

Biodegradation of Selected Petroleum Hydrocarbons Using Indigenous Microorganisms

Oyewole

Olusanya

Akinlade

et al. 2022

Preprint

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Bioremediation make use of microorganisms to remove contaminants from the environment, the use of indigenous microorganisms in the bioremediation of hydrocarbon pollutant to clean up environment has become a valuable technique. This study was aimed to biodegrade some petroleum hydrocarbon using indigenous microorganism from the petroleum contaminated soil. Top soil sample was collected from petroleum contaminated site in Minna, Nigeria. The petroleum was obtained from a local petrol bunk. The indigenous microorganism with biodegrading potential was obtained by plating the aliquot of the soil sample on minimal salt medium containing petroleum as the only source of carbon. The bacterium was identified by cultural and biochemical test and the fungi were identified by both macroscopic and microscopic examinations. The isolates with optimal potential were then subjected to biodegradation for 25 days. A control was setup without the isolate. Three bacterial isolate and four fungi isolate were isolated from the petroleum contaminated site, the bacterial isolates were Corynebacterium xerosis with maximal rate of degradation on day 20 (86%), the control had 12%, Proteus vulgaris maximal rate of biodegradation was on day 35 (80%) while that of control is 40%, and Bacillus subtilis maximal rate of biodegradation was on day 10 (49.73%) while that of control is 2.2%. The fungi isolates were identified as Aspergillus niger, A. versicolar, Trichophyton verrucosum Bodin and Saccharomyces cerivisiae. Trichophyton verrucosum Bodin on day 25 had the highest percentage (88%) of biodegradation in comparison with the control 41%. While A. niger highest biodegradation was observed on day 15 (24%). There was a gradual increase in the count of all the isolate during the course of biodegradation. The result of this study showed that these isolates were able to degrade petroleum hydrocarbon and can be useful for large scale bioremediation of petroleum contaminated soils.

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Newly Isolated Alkane Hydroxylase and Lipase Producing Geobacillus and Anoxybacillus Species Involved in Crude Oil Degradation

Cited by 21 publications

References 41 publications

Polyphasic Analysis Reveals Potential Petroleum Hydrocarbon Degradation and Biosurfactant Production by Rare Biosphere Thermophilic Bacteria From Deception Island, an Active Antarctic Volcano

Polyphasic Analysis Reveals Potential Petroleum Hydrocarbon Degradation and Biosurfactant Production by Rare Biosphere Thermophilic Bacteria From Deception Island, an Active Antarctic Volcano

Microbial Biodegradation of Paraffin Wax in Malaysian Crude Oil Mediated by Degradative Enzymes

Biodegradation of Selected Petroleum Hydrocarbons Using Indigenous Microorganisms

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