Hydroalcoholic extract of Pfaffia glomerata alters the organization of the seminiferous tubules by modulating the oxidative state and the microstructural reorganization of the mice testes

Dias, Fernanda Carolina Ribeiro; Martins, Ana Luiza; Melo, Fabiana Cristina Silveira Alves de; Cupertino, Marli do Carmo; Gomes, Marcos de Lucca Moreira; Oliveira, Jerusa Maria de; Damasceno, E. M.; Silva, Janaína; Otoni, Wagner Campos; Matta, Sérgio Luís Pinto da

doi:10.1016/j.jep.2018.12.047

Cited by 34 publications

(20 citation statements)

References 52 publications

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“…Afterward, it was possible to calculate the number of germ cells in the epithelium, mitotic index, meiotic index, Sertoli cell index, the number of Sertoli cells per testis, the total support capacity of the Sertoli cell, and spermatogenic yield. All formulas were described by Dias et al (2019).…”

Section: Methodsmentioning

confidence: 99%

Green Tea Infusion Ameliorates Histological Damages in Testis and Epididymis of Diabetic Rats

et al. 2021

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Green tea is a popular drink used for therapeutic purposes to mitigate the consequences of diabetes. In this study, we aimed at evaluating the potential of green tea infusion to ameliorate structural and enzymatic damages caused by hyperglycemia in the testis and epididymis of Wistar rats. For that, nondiabetic and streptozotocin-induced diabetic rats (negative control and diabetes control, respectively) received 0.6 mL of water by gavage. Another set of diabetic animals received 100 mg/kg of green tea infusion diluted in 0.6 mL of water/gavage (diabetes + green tea) daily. After 42 days of treatment, the testes and epididymides were removed and processed for histopathological analysis, micromineral determination, and enzymatic assays. The results showed that treatment with green tea infusion preserved the testicular and epididymal histoarchitecture, improving the seminiferous epithelium and the sperm production previously affected by diabetes. Treatment with green tea reduced tissue damages caused by this metabolic condition. Given the severity of hyperglycemia, there was no efficacy of the green tea infusion in maintaining the testosterone levels, antioxidant enzyme activity, and microminerals content. Thus, our findings indicate a protective effect of this infusion on histological parameters, with possible use as a complementary therapy for diabetes.

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Section: Methodsmentioning

confidence: 99%

Green Tea Infusion Ameliorates Histological Damages in Testis and Epididymis of Diabetic Rats

et al. 2021

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“…The tubular (TAr), luminal (LAr) and epithelial (EAr) areas were calculated according to the formulas: TAr = πTR 2 , where TR = tubular radius; LAr = πLR 2 , where LR = luminal radius; EAr = TAr – LAr. The tubule epithelium ratio (TER) was calculated by dividing TAr/EAr (Dias et al., 2019).…”

Section: Methodsmentioning

confidence: 99%

Descriptive morphometry and stereology in accessing the testis structure and function of the marsupial Philander frenatus (Olfers, 1818) (Didelphimorphia: Didelphidae)

Ferreira

Dias

Costa

et al. 2020

Anat Histol Embryol

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Philander frenatus is an important marsupial for the maintenance and conservation of the Atlantic Rainforest, however, it has biological characteristics that are still little explored. The study of the reproductive biology is an important key to understand the species reproductive strategies and to improve the development of conservation and management activities. The present study aimed to conduct a histological and morphometric investigation of the testis structure and function of P. frenatus. The average body and testicular weight were 445 g and 0.74 g, respectively, with a gonadosomatic index of 0.17%. The seminiferous tubules occupying 64.95% of the organ, totalising 9.26 m per gram of testis. The tubulesomatic and epitheliumsomatic indexes were 0.10% and 0.07%, respectively. Philander frenatus showed cell loss of approximately 98% during the proliferative phase and the spermatogenic yield was 10.3 cells. The high loss during the mitotic phase contributed to the low spermatogenic yield. The testicular parenchyma was composed of 35% of intertubular components, one of the highest proportions observed in mammals. Leydig cells were responsible for approximately 25% of the testes, followed by lymphatic space (6.44%), blood vessels and connective tissue (4% together). The organisation of the intertubular components resembles the Fawcett III category. The volume and number of Leydig cell per gram of testis were 2,627.12 µm3 and 91.28 × 106 cells, respectively. High investment in the intertubular compartment, specifically number and volume of Leydig cells in P. frenatus is consistent with territorial behaviour and polygynic mating system, which have greater androgenic capacity.

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“…DNA damage was evaluated in sections of the kidney cortex stained with acridine orange (AO; green) and propidium iodide (PI; red) (Bernas et al, 2005;Suzuki et al, 1997). This fluorescent stain allows to evaluate the DNA damage, as damaged DNA presents red color, marked with PI, and integral DNA is marked in green by the AO (Dias et al, 2019). Digital images were captured using a photomicroscope (Olympus AX 70 TRF, Tokyo, Japan) and analyzed with Image-Pro Plus® 4.5 (Media Cybernetics, Silver Spring, MD) software according to Lima et al (2018).…”

Section: Histopathological and Stereological Analysis And Assessment Of Dna Damagementioning

confidence: 99%

Green tea infusion prevents diabetic nephropathy aggravation in recent-onset type 1 diabetes regardless of glycemic control

Ladeira

Santos

et al. 2021

Journal of Ethnopharmacology

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Hydroalcoholic extract of Pfaffia glomerata alters the organization of the seminiferous tubules by modulating the oxidative state and the microstructural reorganization of the mice testes

Cited by 34 publications

References 52 publications

Green Tea Infusion Ameliorates Histological Damages in Testis and Epididymis of Diabetic Rats

Green Tea Infusion Ameliorates Histological Damages in Testis and Epididymis of Diabetic Rats

Descriptive morphometry and stereology in accessing the testis structure and function of the marsupial Philander frenatus (Olfers, 1818) (Didelphimorphia: Didelphidae)

Green tea infusion prevents diabetic nephropathy aggravation in recent-onset type 1 diabetes regardless of glycemic control

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